Imperial College London
Alternate

Prof Milo Shaffer

Faculty of Natural Sciences, Department of Chemistry

Professor of Materials Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5825m.shaffer Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

401BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Michaeloudes:2020:10.1039/d0na00745e,
author = {Michaeloudes, C and Seiffert, J and Chen, S and Ruenraroengsak, P and Bey, L and Theodorou, IG and Ryan, M and Cui, X and Zhang, J and Shaffer, M and Tetley, T and Porter, AE and Chung, KF},
doi = {10.1039/d0na00745e},
journal = {Nanoscale Advances},
pages = {5635--5647},
title = {Effect of silver nanospheres and nanowires on human airway smooth muscle cells: role of sulfidation},
url = {http://dx.doi.org/10.1039/d0na00745e},
volume = {2},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: The toxicity of inhaled silver nanoparticles on contractile and pro-inflammatory airway smooth muscle cells (ASMCs) that control airway calibre is unknown. We explored the oxidative activities and sulfidation processes of the toxic-inflammatory response. Method: Silver nanospheres (AgNSs) of 20 nm and 50 nm diameter and silver nanowires (AgNWs), short S-AgNWs, 1.5 μm and long L-AgNWs, 10 μm, both 72 nm in diameter were manufactured. We measured their effects on cell proliferation, mitochondrial reactive oxygen species (ROS) release and membrane potential, and also performed electron microscopic studies. Main results and findings: The greatest effects were observed for the smallest particles with the highest specific surface area and greatest solubility that were avidly internalised. ASMCs exposed to 20 nm AgNSs (25 μg mL−1) for 72 hours exhibited a significant decrease in DNA incorporation (−72.4%; p < 0.05), whereas neither the 50 nm AgNSs nor the s-AgNWs altered DNA synthesis or viability. There was a small reduction in ASMC proliferation for the smaller AgNS, although Ag+ at 25 μL mL−1 reduced DNA synthesis by 93.3% (p < 0.001). Mitochondrial potential was reduced by both Ag+ (25 μg mL−1) by 47.1% and 20 nm Ag NSs (25 μg mL−1) by 40.1% (both at p < 0.05), but was not affected by 50 nm AgNSs and the AgNWs. None of the samples showed a change in ROS toxicity. However, malondialdehyde release, associated with greater total ROS, was observed for all AgNPs, to an extent following the geometric size (20 nm AgNS: 213%, p < 0.01; 50 nm AgNS: 179.5%, p < 0.01 and L-AgNWs by 156.2%, p < 0.05). The antioxidant, N-acetylcysteine, prevented the reduction in mitochondrial potential caused by 20 nm AgNSs. The smaller nanostructures were internalised and dissolved within the ASMCs with the formation of non-reactive silver sulphide (Ag2S) on their surface, but with very little uptake of L-AgNWs. When A
AU  - Michaeloudes,C
AU  - Seiffert,J
AU  - Chen,S
AU  - Ruenraroengsak,P
AU  - Bey,L
AU  - Theodorou,IG
AU  - Ryan,M
AU  - Cui,X
AU  - Zhang,J
AU  - Shaffer,M
AU  - Tetley,T
AU  - Porter,AE
AU  - Chung,KF
DO  - 10.1039/d0na00745e
EP  - 5647
PY  - 2020///
SN  - 2516-0230
SP  - 5635
TI  - Effect of silver nanospheres and nanowires on human airway smooth muscle cells: role of sulfidation
T2  - Nanoscale Advances
UR  - http://dx.doi.org/10.1039/d0na00745e
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000599127600015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/NA/D0NA00745E#!divAbstract
UR  - http://hdl.handle.net/10044/1/88348
VL  - 2
ER  -
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