Imperial College London
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Professor Michael A. ("Mike") Skinner

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor in Virology
 
 
 
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Contact

 

+44 (0)20 7594 3938m.skinner Website

 
 
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Assistant

 

Mrs Yasmin Mallu +44 (0)20 7594 3972

 
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Location

 

315Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Giotis:2019:10.3390/genes10030237,
author = {Giotis, E and Montillet, G and Pain, B and Skinner, M},
doi = {10.3390/genes10030237},
journal = {Genes},
title = {Chicken embryonic-stem cells are permissive to poxvirus recombinant vaccine vectors},
url = {http://dx.doi.org/10.3390/genes10030237},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The discovery of mammalian pluripotent embryonic stem cells (ESC) has revolutionised cell research and regenerative medicine. More recently discovered chicken ESC (cESC), though less intensively studied, are increasingly popular as vaccine substrates due to a dearth of avian cell lines. Information on the comparative performance of cESC with common vaccine viruses is limited. Using RNA-sequencing, we compared cESC transcriptional programmes elicited by stimulation with chicken type I interferon or infection with vaccine viruses routinely propagated in primary chicken embryo fibroblasts (CEF). We used poxviruses (fowlpox virus (FWPV) FP9, canarypox virus (CNPV), and modified vaccinia virus Ankara (MVA)) and a birnavirus (infectious bursal disease virus (IBDV) PBG98). Interferon-stimulated genes (ISGs) were induced in cESC to levels comparable to those in CEF and immortalised chicken fibroblast DF-1 cells. cESC are permissive (with distinct host transcriptional responses) to MVA, FP9, and CNPV but, surprisingly, not to PBG98. MVA, CNPV, and FP9 suppressed innate immune responses, while PBG98 induced a subset of ISGs. Dysregulation of signalling pathways (i.e., NFκB, TRAF) was observed, which might affect immune responses and viral replication. In conclusion, we show that cESC are an attractive alternative substrate to study and propagate poxvirus recombinant vaccine vectors.
AU  - Giotis,E
AU  - Montillet,G
AU  - Pain,B
AU  - Skinner,M
DO  - 10.3390/genes10030237
PY  - 2019///
SN  - 2073-4425
TI  - Chicken embryonic-stem cells are permissive to poxvirus recombinant vaccine vectors
T2  - Genes
UR  - http://dx.doi.org/10.3390/genes10030237
UR  - http://hdl.handle.net/10044/1/69511
VL  - 10
ER  -
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