Imperial College London

ProfessorMichaelSternberg

Faculty of Natural SciencesDepartment of Life Sciences

Director Centre for Bioinformatics
 
 
 
//

Contact

 

+44 (0)20 7594 5212m.sternberg Website

 
 
//

Location

 

306Sir Ernst Chain BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Wass:2012:10.1017/S0031182012000133,
author = {Wass, MN and Stanway, R and Blagborough, AM and Lal, K and Prieto, JH and Raine, D and Sternberg, MJE and Talman, AM and Tomley, F and Yates, J and Sinden, RE},
doi = {10.1017/S0031182012000133},
journal = {Parasitology},
pages = {1131--1145},
title = {Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls},
url = {http://dx.doi.org/10.1017/S0031182012000133},
volume = {139},
year = {2012}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e.gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of theproteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarisekey biological interpretations of the data, we also try to identify key methodological constraints we have met, only some ofwhich we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functionalunderstanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction ofprotein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simplestructural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported byongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.
AU - Wass,MN
AU - Stanway,R
AU - Blagborough,AM
AU - Lal,K
AU - Prieto,JH
AU - Raine,D
AU - Sternberg,MJE
AU - Talman,AM
AU - Tomley,F
AU - Yates,J
AU - Sinden,RE
DO - 10.1017/S0031182012000133
EP - 1145
PY - 2012///
SN - 1469-8161
SP - 1131
TI - Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls
T2 - Parasitology
UR - http://dx.doi.org/10.1017/S0031182012000133
UR - http://hdl.handle.net/10044/1/31224
VL - 139
ER -