Imperial College London

ProfessorMichaelSternberg

Faculty of Natural SciencesDepartment of Life Sciences

Director Centre for Bioinformatics
 
 
 
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Contact

 

+44 (0)20 7594 5212m.sternberg Website

 
 
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Location

 

306Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Metherell:2016:10.1002/humu.23046,
author = {Metherell, LA and Guerra-Assunção, JA and Sternberg, M and David, A},
doi = {10.1002/humu.23046},
journal = {Human Mutation},
pages = {1074--1084},
title = {Three-dimensional model of human Nicotinamide Nucleotide Transhydrogenase (NNT) and sequence-structure analysis of its disease-causing variations},
url = {http://dx.doi.org/10.1002/humu.23046},
volume = {37},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Defective mitochondrial proteins are emerging as major contributors to human disease. Nicotinamide nucleotide transhydrogenase (NNT), a widely expressed mitochondrial protein, has a crucial role in the defence against oxidative stress. NNT variations have recently been reported in patients with familial glucocorticoid deficiency (FGD) and in patients with heart failure. Moreover, knockout animal models suggest that NNT has a major role in diabetes mellitus and obesity. In this study, we used experimental structures of bacterial transhydrogenases to generate a structural model of human NNT (H-NNT). Structure-based analysis allowed the identification of H-NNT residues forming the NAD binding site, the proton canal and the large interaction site on the H-NNT dimer. In addition, we were able to identify key motifs that allow conformational changes adopted by domain III in relation to its functional status, such as the flexible linker between domains II and III and the salt bridge formed by H-NNT Arg882 and Asp830. Moreover, integration of sequence and structure data allowed us to study the structural and functional effect of deleterious amino acid substitutions causing FGD and left ventricular non-compaction cardiomyopathy. In conclusion, interpretation of the function–structure relationship of H-NNT contributes to our understanding of mitochondrial disorders.
AU - Metherell,LA
AU - Guerra-Assunção,JA
AU - Sternberg,M
AU - David,A
DO - 10.1002/humu.23046
EP - 1084
PY - 2016///
SN - 1098-1004
SP - 1074
TI - Three-dimensional model of human Nicotinamide Nucleotide Transhydrogenase (NNT) and sequence-structure analysis of its disease-causing variations
T2 - Human Mutation
UR - http://dx.doi.org/10.1002/humu.23046
UR - http://hdl.handle.net/10044/1/34524
VL - 37
ER -