Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hansel:2019:10.1021/acsnano.8b06998,
author = {Hansel, C and Crowder, S and Cooper, S and Gopal, S and Pardelha, da Cruz J and De, Oliveira Martins L and Keller, D and Rothery, S and Becce, M and Cass, A and Bakal, C and Chiappini, C and Stevens, M},
doi = {10.1021/acsnano.8b06998},
journal = {ACS Nano},
pages = {2913--2019},
title = {Nanoneedle-mediated stimulation of cell mechanotransduction machinery},
url = {http://dx.doi.org/10.1021/acsnano.8b06998},
volume = {13},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Biomaterial substrates can be engineered to present topographical signals to cells which, through interactions between the material and active components of the cell membrane, regulate key cellular processes and guide cell fate decisions. However, targeting mechanoresponsive elements that reside within the intracellular domain is a concept that has only recently emerged. Here, we show that mesoporous silicon nanoneedle arrays interact simultaneously with the cell membrane, cytoskeleton, and nucleus of primary human cells, generating distinct responses at each of these cellular compartments. Specifically, nanoneedles inhibit focal adhesion maturation at the membrane, reduce tension in the cytoskeleton, and lead to remodeling of the nuclear envelope at sites of impingement. The combined changes in actin cytoskeleton assembly, expression and segregation of the nuclear lamina, and localization of Yes-associated protein (YAP) correlate differently from what is canonically observed upon stimulation at the cell membrane, revealing that biophysical cues directed to the intracellular space can generate heretofore unobserved mechanosensory responses. These findings highlight the ability of nanoneedles to study and direct the phenotype of large cell populations simultaneously, through biophysical interactions with multiple mechanoresponsive components.
AU - Hansel,C
AU - Crowder,S
AU - Cooper,S
AU - Gopal,S
AU - Pardelha,da Cruz J
AU - De,Oliveira Martins L
AU - Keller,D
AU - Rothery,S
AU - Becce,M
AU - Cass,A
AU - Bakal,C
AU - Chiappini,C
AU - Stevens,M
DO - 10.1021/acsnano.8b06998
EP - 2019
PY - 2019///
SN - 1936-0851
SP - 2913
TI - Nanoneedle-mediated stimulation of cell mechanotransduction machinery
T2 - ACS Nano
UR - http://dx.doi.org/10.1021/acsnano.8b06998
UR - http://hdl.handle.net/10044/1/68052
VL - 13
ER -