Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2019:10.1021/acsami.9b04663,
author = {Wang, Y and Kim, E and Lin, Y and Kim, N and Kit-Anan, W and Gopal, S and Agarwal, S and Howes, P and Stevens, M},
doi = {10.1021/acsami.9b04663},
journal = {ACS Applied Materials and Interfaces},
title = {Rolling circle transcription-amplified hierarchically structured organic-inorganic hybrid RNA flowers for enzyme immobilization},
url = {http://dx.doi.org/10.1021/acsami.9b04663},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Programmable nucleic acids have emerged as powerful building blocks for the bottom-up fabrication of two- or three-dimensional nano- and microsized constructs. Here we describe the construction of organic–inorganic hybrid RNA flowers (hRNFs) via rolling circle transcription (RCT), an enzyme-catalyzed nucleic acid amplification reaction. These hRNFs are highly adaptive structures with controlled sizes, specific nucleic acid sequences, and a highly porous nature. We demonstrated that hRNFs are applicable as potential biological platforms, where the hRNF scaffold can be engineered for versatile surface functionalization and the inorganic component (magnesium ions) can serve as an enzyme cofactor. For surface functionalization, we proposed robust and straightforward approaches including in situ synthesis of functional hRNFs and postfunctionalization of hRNFs that enable facile conjugation with various biomolecules and nanomaterials (i.e., proteins, enzymes, organic dyes, inorganic nanoparticles) using selective chemistries (i.e., avidin–biotin interaction, copper-free click reaction). In particular, we showed that hRNFs can serve as soft scaffolds for β-galactosidase immobilization and greatly enhance enzymatic activity and stability. Therefore, the proposed concepts and methodologies are not only fundamentally interesting when designing RNA scaffolds or RNA bionanomaterials assembled with enzymes but also have significant implications on their future utilization in biomedical applications ranging from enzyme cascades to biosensing and drug delivery.
AU - Wang,Y
AU - Kim,E
AU - Lin,Y
AU - Kim,N
AU - Kit-Anan,W
AU - Gopal,S
AU - Agarwal,S
AU - Howes,P
AU - Stevens,M
DO - 10.1021/acsami.9b04663
PY - 2019///
SN - 1944-8244
TI - Rolling circle transcription-amplified hierarchically structured organic-inorganic hybrid RNA flowers for enzyme immobilization
T2 - ACS Applied Materials and Interfaces
UR - http://dx.doi.org/10.1021/acsami.9b04663
UR - http://hdl.handle.net/10044/1/70877
ER -