Imperial College London

Professor Molly Stevens

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
//

Contact

 

+44 (0)20 7594 6804m.stevens

 
 
//

Location

 

208Royal School of MinesSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{LaPointe:2013:10.1371/journal.pone.0082035,
author = {LaPointe, VLS and Verpoorte, A and Stevens, MM},
doi = {10.1371/journal.pone.0082035},
journal = {PLoS ONE},
pages = {1--13},
title = {The changing integrin expression and a role for integrin beta 8 in the chondrogenic differentiation of mesenchymal stem cells},
url = {http://dx.doi.org/10.1371/journal.pone.0082035},
volume = {8},
year = {2013}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Many cartilage tissue engineering approaches aim to differentiate human mesenchymal stem cells (hMSCs) into chondrocytes and develop cartilage in vitro by targeting cell-matrix interactions. We sought to better inform the design of cartilage tissue engineering scaffolds by understanding how integrin expression changes during chondrogenic differentiation. In three models of in vitro chondrogenesis, we studied the temporal change of cartilage phenotype markers and integrin subunits during the differentiation of hMSCs. We found that transcript expression of most subunits was conserved across the chondrogenesis models, but was significantly affected by the time-course of differentiation. In particular, ITGB8 was up-regulated and its importance in chondrogenesis was further established by a knockdown of integrin β8, which resulted in a non-hyaline cartilage phenotype, with no COL2A1 expression detected. In conclusion, we performed a systematic study of the temporal changes of integrin expression during chondrogenic differentiation in multiple chondrogenesis models, and revealed a role for integrin β8 in chondrogenesis. This work enhances our understanding of the changing adhesion requirements of hMSCs during chondrogenic differentiation and underlines the importance of integrins in establishing a cartilage phenotype.
AU - LaPointe,VLS
AU - Verpoorte,A
AU - Stevens,MM
DO - 10.1371/journal.pone.0082035
EP - 13
PY - 2013///
SN - 1932-6203
SP - 1
TI - The changing integrin expression and a role for integrin beta 8 in the chondrogenic differentiation of mesenchymal stem cells
T2 - PLoS ONE
UR - http://dx.doi.org/10.1371/journal.pone.0082035
UR - http://hdl.handle.net/10044/1/49623
VL - 8
ER -