Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Fuhrmann:2015:10.1016/j.jconrel.2014.11.029,
author = {Fuhrmann, G and Serio, A and Mazo, M and Nair, R and Stevens, MM},
doi = {10.1016/j.jconrel.2014.11.029},
journal = {Journal of Controlled Release},
pages = {35--44},
title = {Active loading into extracellular vesicles significantly improves the cellular uptake and photodynamic effect of porphyrins},
url = {http://dx.doi.org/10.1016/j.jconrel.2014.11.029},
volume = {205},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Extracellular vesicles (EVs) are phospholipid-based particles endogenously produced by cells. Their natural composition and selective cell interactions make them promising drug carriers. However, in order to harness their properties, efficient exogenous drug encapsulation methods need to be investigated. Here, EVs from various cellular origins (endothelial, cancer and stem cells) were produced and characterised for size and composition. Porphyrins of different hydrophobicities were employed as model drugs and encapsulated into EVs using various passive and active methods (electroporation, saponin, extrusion and dialysis). Hydrophobic compounds loaded very efficiently into EVs and at significantly higher amounts than into standard liposomes composed of phosphocholine and cholesterol using passive incubation. Moreover, loading into EVs significantly increased the cellular uptake by > 60% and the photodynamic effect of hydrophobic porphyrins in vitro compared to free or liposome encapsulated drug. The active encapsulation techniques, with the saponin-assisted method in particular, allowed an up to 11 fold higher drug loading of hydrophilic porphyrins compared to passive methods. EVs loaded with hydrophilic porphyrins induced a stronger phototoxic effect than free drug in a cancer cell model. Our findings create a firm basis for the development of EVs as smart drug carriers based on straightforward and transferable methods.
AU - Fuhrmann,G
AU - Serio,A
AU - Mazo,M
AU - Nair,R
AU - Stevens,MM
DO - 10.1016/j.jconrel.2014.11.029
EP - 44
PY - 2015///
SN - 1873-4995
SP - 35
TI - Active loading into extracellular vesicles significantly improves the cellular uptake and photodynamic effect of porphyrins
T2 - Journal of Controlled Release
UR - http://dx.doi.org/10.1016/j.jconrel.2014.11.029
UR - http://hdl.handle.net/10044/1/45394
VL - 205
ER -