Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
//

Contact

 

+44 (0)20 7594 6804m.stevens

 
 
//

Location

 

208Royal School of MinesSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Campagnolo:2015:10.1016/j.biomaterials.2015.04.055,
author = {Campagnolo, P and Tsai, TN and Hong, X and Kirton, JP and So, PW and Margariti, A and Di, Bernardini E and Wong, MM and Hu, Y and Stevens, MM and Xu, Q},
doi = {10.1016/j.biomaterials.2015.04.055},
journal = {Biomaterials},
pages = {53--61},
title = {c-Kit+ progenitors generate vascular cells for tissue-engineered grafts through modulation of the Wnt/Klf4 pathway.},
url = {http://dx.doi.org/10.1016/j.biomaterials.2015.04.055},
volume = {60},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The development of decellularised scaffolds for small diameter vascular grafts is hampered by their limited patency, due to the lack of luminal cell coverage by endothelial cells (EC) and to the low tone of the vessel due to absence of a contractile smooth muscle cells (SMC). In this study, we identify a population of vascular progenitor c-Kit+/Sca-1- cells available in large numbers and derived from immuno-privileged embryonic stem cells (ESCs). We also define an efficient and controlled differentiation protocol yielding fully to differentiated ECs and SMCs in sufficient numbers to allow the repopulation of a tissue engineered vascular graft. When seeded ex vivo on a decellularised vessel, c-Kit+/Sca-1-derived cells recapitulated the native vessel structure and upon in vivo implantation in the mouse, markedly reduced neointima formation and mortality, restoring functional vascularisation. We showed that Krüppel-like transcription factor 4 (Klf4) regulates the choice of differentiation pathway of these cells through β-catenin activation and was itself regulated by the canonical Wnt pathway activator lithium chloride. Our data show that ESC-derived c-Kit+/Sca-1-cells can be differentiated through a Klf4/β-catenin dependent pathway and are a suitable source of vascular progenitors for the creation of superior tissue-engineered vessels from decellularised scaffolds.
AU - Campagnolo,P
AU - Tsai,TN
AU - Hong,X
AU - Kirton,JP
AU - So,PW
AU - Margariti,A
AU - Di,Bernardini E
AU - Wong,MM
AU - Hu,Y
AU - Stevens,MM
AU - Xu,Q
DO - 10.1016/j.biomaterials.2015.04.055
EP - 61
PY - 2015///
SN - 1878-5905
SP - 53
TI - c-Kit+ progenitors generate vascular cells for tissue-engineered grafts through modulation of the Wnt/Klf4 pathway.
T2 - Biomaterials
UR - http://dx.doi.org/10.1016/j.biomaterials.2015.04.055
UR - http://hdl.handle.net/10044/1/23821
VL - 60
ER -