Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine



+44 (0)20 7594 6804m.stevens




208Royal School of MinesSouth Kensington Campus






BibTex format

author = {Kallepitis, C and Bergholt, MS and Mazo, MM and Leonardo, V and Skaalure, SC and Maynard, SA and Stevens, MM},
doi = {10.1038/ncomms14843},
journal = {Nature Communications},
title = {Quantitative volumetric Raman imaging of three dimensional cell cultures},
url = {},
volume = {8},
year = {2017}

RIS format (EndNote, RefMan)

AB - The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in 3D cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
AU - Kallepitis,C
AU - Bergholt,MS
AU - Mazo,MM
AU - Leonardo,V
AU - Skaalure,SC
AU - Maynard,SA
AU - Stevens,MM
DO - 10.1038/ncomms14843
PY - 2017///
SN - 2041-1723
TI - Quantitative volumetric Raman imaging of three dimensional cell cultures
T2 - Nature Communications
UR -
UR -
VL - 8
ER -