Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2017:10.1016/j.talanta.2017.05.015,
author = {Wang, C and Lin, Y and Hsu, C and Amdursky, N and Spicer, C and Stevens, MM},
doi = {10.1016/j.talanta.2017.05.015},
journal = {Talanta},
pages = {44--50},
title = {Probing amylin fibrillation at an early stage via a tetracysteine-recognising fluorophore},
url = {http://dx.doi.org/10.1016/j.talanta.2017.05.015},
volume = {173},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Amyloid fibrillation is a nucleation-dependent process known be involved in the development of more than 20 progressive and chronic diseases. The detection of amyloid formation at the nucleation stage can greatly advance early diagnoses and treatment of diseases. In this work, we developed a new assay for the early detection of amylin fibrillation using the biarsenical dye 4,5-bis(1,3,2-dithiarsolan-2-yl)fluorescein (FlAsH), which could recognise tetracysteine motifs and transform from non-fluorescent form into strongly fluorescent complexes. Due to the close proximity of two cysteine residues within the hydrophilic domain of amylin, a non-contiguous tetracysteine motif can form upon amylin dimerisation or oligomerisation, which can be recognised by FlAsH and emit strong fluorescence. This enables us to report the nucleation-growth process of amylin without modification of the protein sequence. We showed that the use of this assay not only allowed the tracking of initial nucleation events, but also enabled imaging of amyloid fibrils and investigation of the effects of amyloid inhibitor/modulator toward amylin fibrillation.
AU - Wang,C
AU - Lin,Y
AU - Hsu,C
AU - Amdursky,N
AU - Spicer,C
AU - Stevens,MM
DO - 10.1016/j.talanta.2017.05.015
EP - 50
PY - 2017///
SN - 1873-3573
SP - 44
TI - Probing amylin fibrillation at an early stage via a tetracysteine-recognising fluorophore
T2 - Talanta
UR - http://dx.doi.org/10.1016/j.talanta.2017.05.015
UR - http://hdl.handle.net/10044/1/48483
VL - 173
ER -