Imperial College London

Professor Molly Stevens

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lim:2013:10.5999/aps.2013.40.6.676,
author = {Lim, EH and Sardinha, JP and Myers, S and Stevens, M},
doi = {10.5999/aps.2013.40.6.676},
journal = {Archives of Plastic Surgery},
pages = {676--686},
title = {Latent transforming growth factor-beta1 functionalised electrospun scaffolds promote human cartilage differentiation: Towards an engineered cartilage construct},
url = {http://dx.doi.org/10.5999/aps.2013.40.6.676},
volume = {40},
year = {2013}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Background To overcome the potential drawbacks of a short half-life and dose-related adverse effects of using active transforming growth factor-beta 1 for cartilage engineering, a cell-mediated latent growth factor activation strategy was developed incorporating latent transforming growth factor-β1 (LTGF) into an electrospun poly(L-lactide) scaffold. Methods The electrospun scaffold was surface modified with NH3 plasma and biofunctionalised with LTGF to produce both random and orientated biofunctionalised electrospun scaffolds. Scaffold surface chemical analysis and growth factor bioavailability assays were performed. In vitro biocompatibility and human nasal chondrocyte gene expression with these biofunctionalised electrospun scaffold templates were assessed. In vivo chondrogenic activity and chondrocyte gene expression were evaluated in athymic rats. Results Chemical analysis demonstrated that LTGF anchored to the scaffolds was available for enzymatic, chemical and cell activation. The biofunctionalised scaffolds were non-toxic. Gene expression suggested chondrocyte re-differentiation after 14 days in culture. By 6 weeks, the implanted biofunctionalised scaffolds had induced highly passaged chondrocytes to re-express Col2A1 and produce type II collagen. Conclusions We have demonstrated a proof of concept for cell-mediated activation of anchored growth factors using a novel biofunctionalised scaffold in cartilage engineering. This presents a platform for development of protein delivery systems and for tissue engineering. © 2013 The Korean Society of Plastic and Reconstructive Surgeons.
AU - Lim,EH
AU - Sardinha,JP
AU - Myers,S
AU - Stevens,M
DO - 10.5999/aps.2013.40.6.676
EP - 686
PY - 2013///
SN - 2234-6163
SP - 676
TI - Latent transforming growth factor-beta1 functionalised electrospun scaffolds promote human cartilage differentiation: Towards an engineered cartilage construct
T2 - Archives of Plastic Surgery
UR - http://dx.doi.org/10.5999/aps.2013.40.6.676
VL - 40
ER -