Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

332 results found

Kim E, Agarwal S, Kim N, Hage FS, Gelmi A, Stevens Met al., Bio-Inspired Fabrication of DNA-Inorganic Hybrid Composites Using Synthetic DNA, ACS Nano

JOURNAL ARTICLE

Reznikov N, Boughton OR, Ghouse S, Weston AE, Collinson L, Blunn GW, Jeffers JRT, Cobb JP, Stevens MMet al., 2019, Individual response variations in scaffold-guided bone regeneration are determined by independent strain- and injury-induced mechanisms, BIOMATERIALS, Vol: 194, Pages: 183-194, ISSN: 0142-9612

JOURNAL ARTICLE

Lin Y, Mazo MM, Skaalure SC, Thomas MR, Schultz SR, Stevens MMet al., 2019, Activatable cell-biomaterial interfacing with photo-caged peptides, CHEMICAL SCIENCE, Vol: 10, Pages: 1158-1167, ISSN: 2041-6520

JOURNAL ARTICLE

Gopal S, Chiappini C, Penders J, Leonardo V, Seong H, Rothery S, Korchev Y, Shevchuk A, Stevens MMet al., 2019, Porous Silicon Nanoneedles Modulate Endocytosis to Deliver Biological Payloads., Adv Mater

Owing to their ability to efficiently deliver biological cargo and sense the intracellular milieu, vertical arrays of high aspect ratio nanostructures, known as nanoneedles, are being developed as minimally invasive tools for cell manipulation. However, little is known of the mechanisms of cargo transfer across the cell membrane-nanoneedle interface. In particular, the contributions of membrane piercing, modulation of membrane permeability and endocytosis to cargo transfer remain largely unexplored. Here, combining state-of-the-art electron and scanning ion conductance microscopy with molecular biology techniques, it is shown that porous silicon nanoneedle arrays concurrently stimulate independent endocytic pathways which contribute to enhanced biomolecule delivery into human mesenchymal stem cells. Electron microscopy of the cell membrane at nanoneedle sites shows an intact lipid bilayer, accompanied by an accumulation of clathrin-coated pits and caveolae. Nanoneedles enhance the internalization of biomolecular markers of endocytosis, highlighting the concurrent activation of caveolae- and clathrin-mediated endocytosis, alongside macropinocytosis. These events contribute to the nanoneedle-mediated delivery (nanoinjection) of nucleic acids into human stem cells, which distribute across the cytosol and the endolysosomal system. This data extends the understanding of how nanoneedles modulate biological processes to mediate interaction with the intracellular space, providing indications for the rational design of improved cell-manipulation technologies.

JOURNAL ARTICLE

Lin Y, Penna M, Thomas MR, Wojciechowski JP, Leonardo V, Wang Y, Pashuck ET, Yarovsky I, Stevens MMet al., 2019, Residue-Specific Solvation-Directed Thermodynamic and Kinetic Control over Peptide Self-Assembly with 1D/2D Structure Selection., ACS Nano

Understanding the self-organization and structural transformations of molecular ensembles is important to explore the complexity of biological systems. Here, we illustrate the crucial role of cosolvents and solvation effects in thermodynamic and kinetic control over peptide association into ultrathin Janus nanosheets, elongated nanobelts, and amyloid-like fibrils. We gained further insight into the solvation-directed self-assembly (SDSA) by investigating residue-specific peptide solvation using molecular dynamics modeling. We proposed the preferential solvation of the aromatic and alkyl domains on the peptide backbone and protofibril surface, which results in volume exclusion effects and restricts the peptide association between hydrophobic walls. We explored the SDSA phenomenon in a library of cosolvents (protic and aprotic), where less polar cosolvents were found to exert a stronger influence on the energetic balance at play during peptide propagation. By tailoring cosolvent polarity, we were able to achieve precise control of the peptide nanostructures with 1D/2D shape selection. We also illustrated the complexity of the SDSA system with pathway-dependent peptide aggregation, where two self-assembly states ( i.e., thermodynamic equilibrium state and kinetically trapped state) from different sample preparation methods were obtained.

JOURNAL ARTICLE

Armstrong JPK, Maynard SA, Pence IJ, Franklin AC, Drinkwater BW, Stevens MMet al., 2019, Spatiotemporal quantification of acoustic cell patterning using Voronoï tessellation., Lab Chip

Acoustic patterning using ultrasound standing waves has recently emerged as a potent biotechnology enabling the remote generation of ordered cell systems. This capability has opened up exciting opportunities, for example, in guiding the development of organoid cultures or the organization of complex tissues. The success of these studies is often contingent on the formation of tightly-packed and uniform cell arrays; however, a number of factors can act to disrupt or prevent acoustic patterning. Yet, to the best of our knowledge, there has been no comprehensive assessment of the quality of acoustically-patterned cell populations. In this report we use a mathematical approach, known as Voronoï tessellation, to generate a series of metrics that can be used to measure the effect of cell concentration, pressure amplitude, ultrasound frequency and biomaterial viscosity upon the quality of acoustically-patterned cell systems. Moreover, we extend this approach towards the characterization of spatiotemporal processes, namely, the acoustic patterning of cell suspensions and the migration of patterned, adherent cell clusters. This strategy is simple, unbiased and highly informative, and we anticipate that the methods described here will provide a systematic framework for all stages of acoustic patterning, including the robust quality control of devices, statistical comparison of patterning conditions, the quantitative exploration of parameter limits and the ability to track patterned tissue formation over time.

JOURNAL ARTICLE

Lin Y, Charchar P, Christofferson AJ, Thomas MR, Todorova N, Mazo MM, Chen Q, Doutch J, Richardson R, Yarovs I, Stevens MMet al., 2018, Surface Dynamics and Ligand-Core Interactions of Quantum Sized Photoluminescent Gold Nanoclusters, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 140, Pages: 18217-18226, ISSN: 0002-7863

JOURNAL ARTICLE

Pujari-Palmer M, Guo H, Wenner D, Autefage H, Spicer CD, Stevens MM, Omar O, Thomsen P, Eden M, Insley G, Procter P, Engqvist Het al., 2018, A Novel Class of Injectable Bioceramics That Glue Tissues and Biomaterials, MATERIALS, Vol: 11, ISSN: 1996-1944

JOURNAL ARTICLE

Jumeaux C, Kim E, Howes P, Kim H, Chandrawati R, Stevens Met al., 2018, Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion, Nanoscale Advances, ISSN: 2516-0230

We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.

JOURNAL ARTICLE

Armstrong JPK, Puetzer JL, Serio A, Guex AG, Kapnisi M, Breant A, Zong Y, Assal V, Skaalure SC, King O, Murty T, Meinert C, Franklin AC, Bassindale PG, Nichols MK, Terracciano CM, Hutmacher DW, Drinkwater BW, Klein TJ, Perriman AW, Stevens MMet al., 2018, Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning, ADVANCED MATERIALS, Vol: 30, ISSN: 0935-9648

JOURNAL ARTICLE

Penders J, Pence IJ, Horgan CC, Bergholt MS, Wood CS, Najer A, Kauscher U, Nagelkerke A, Stevens MMet al., 2018, Single Particle Automated Raman Trapping Analysis, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Gray ER, Bain R, Varsaneux O, Peeling RW, Stevens MM, McKendry RAet al., 2018, p24 revisited: a landscape review of antigen detection for early HIV diagnosis, AIDS, Vol: 32, Pages: 2089-2102, ISSN: 0269-9370

JOURNAL ARTICLE

Amdursky N, Mazo MM, Thomas MR, Humphrey EJ, Puetzer JL, St-Pierre J-P, Skaalure SC, Richardson RM, Terracciano CM, Stevens MMet al., 2018, Elastic serum-albumin based hydrogels: mechanism of formation and application in cardiac tissue engineering, JOURNAL OF MATERIALS CHEMISTRY B, Vol: 6, Pages: 5604-5612, ISSN: 2050-750X

JOURNAL ARTICLE

Wood CS, Thomas M, Budd J, Mashamba-Thompson TP, Herbst K, Pillay D, Peeling RW, Johnson AM, McKendry RA, Stevens Met al., Taking connected mobile-health diagnostics of infectious diseases to the field., Nature, ISSN: 0028-0836

Mobile Health or mHealth - The application of mobile devices, their componentsand related technologies to healthcare is improving patients’ access to treatment andadvice. Now, in combination with connected diagnostic devices it offers new possibilitiesto diagnose, track and control infectious diseases and improve health systemefficiencies. In this context we look at these technologies and highlight their promise butalso the challenges in realising their potential to increase patient access to testing, aid intheir treatment and improve the capability of public health authorities to monitoroutbreaks, implement responses, and assess the impact of interventions across theworld.

JOURNAL ARTICLE

Li C, Armstrong JPK, Pence IJ, Kit-Anan W, Puetzer JL, Carreira SC, Moore AC, Stevens MMet al., 2018, Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering, BIOMATERIALS, Vol: 176, Pages: 24-33, ISSN: 0142-9612

JOURNAL ARTICLE

Faria M, Bjornmalm M, Thurecht KJ, Kent SJ, Parton RG, Kavallaris M, Johnston APR, Gooding JJ, Corrie SR, Boyd BJ, Thordarson P, Whittaker AK, Stevens MM, Prestidge CA, Porter CJH, Parak WJ, Davis TP, Crampin EJ, Caruso Fet al., 2018, Minimum information reporting in bio-nano experimental literature, NATURE NANOTECHNOLOGY, Vol: 13, Pages: 777-785, ISSN: 1748-3387

JOURNAL ARTICLE

Tallia F, Russo L, Li S, Orrin ALH, Shi X, Chen S, Steele JAM, Meille S, Chevalier J, Lee PD, Stevens MM, Cipolla L, Jones JRet al., 2018, Bouncing and 3D printable hybrids with self-healing properties, MATERIALS HORIZONS, Vol: 5, Pages: 849-860, ISSN: 2051-6347

JOURNAL ARTICLE

Sigmundsson K, Ojala JRM, Ohman MK, Osterholm A-M, Moreno-Moral A, Domogatskaya A, Chong LY, Sun Y, Chai X, Steele JAM, George B, Patarroyo M, Nilsson A-S, Rodin S, Ghosh S, Stevens MM, Petretto E, Tryggvason Ket al., 2018, Culturing functional pancreatic islets on alpha 5-Iaminins and curative transplantation , to diabetic mice, MATRIX BIOLOGY, Vol: 70, Pages: 5-19, ISSN: 0945-053X

JOURNAL ARTICLE

Wang Y, Howes PD, Kim E, Spicer CD, Thomas MR, Lin Y, Crowder SW, Pence IJ, Stevens MMet al., 2018, Duplex-Specific Nuclease-Amplified Detection of MicroRNA Using Compact Quantum Dot-DNA Conjugates, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 28290-28300, ISSN: 1944-8244

JOURNAL ARTICLE

Spicer CD, Pashuck ET, Stevens MM, 2018, Achieving Controlled Biomolecule-Biomaterial Conjugation, CHEMICAL REVIEWS, Vol: 118, Pages: 7702-7743, ISSN: 0009-2665

JOURNAL ARTICLE

Holme MN, Rashid MH, Thomas MR, Barriga HMG, Herpoldt K-L, Heenan RK, Dreiss CA, Banuelos JL, Xie H-N, Yarovsky I, Stevens MMet al., 2018, Fate of Liposomes in the Presence of Phospholipase C and D: From Atomic to Supramolecular Lipid Arrangement, ACS CENTRAL SCIENCE, Vol: 4, Pages: 1023-1030, ISSN: 2374-7943

JOURNAL ARTICLE

Creamer A, Wood CS, Howes PD, Casey A, Cong S, Marsh AV, Godin R, Panidi J, Anthopoulos TD, Burgess CH, Wu T, Fei Z, Hamilton I, McLachlan MA, Stevens MM, Heeney Met al., 2018, Post-polymerisation functionalisation of conjugated polymer backbones and its application in multifunctional emissive nanoparticles, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Holme MN, Rana S, Barriga HMG, Kauscher U, Brooks NJ, Stevens MMet al., 2018, A Robust Liposomal Platform for Direct Colorimetric Detection of Sphingomyelinase Enzyme and Inhibitors, ACS NANO, Vol: 12, Pages: 8197-8207, ISSN: 1936-0851

JOURNAL ARTICLE

Barriga HMG, Holme MN, Stevens MM, 2018, Cubosomes: The Next Generation of Smart Lipid Nanoparticles?, Angew Chem Int Ed Engl

Cubosomes are highly stable nanoparticles formed from the lipid cubic phase and stabilized by a polymer based outer corona. Bicontinuous lipid cubic phases consist of a single lipid bilayer that forms a continuous periodic membrane lattice structure with pores formed by two interwoven water channels. Cubosome composition can be tuned to engineer pore sizes or include bioactive lipids, the polymer outer corona can be used for targeting and they are highly stable under physiological conditions. Compared to liposomes, the structure provides a significantly higher membrane surface area for loading of membrane proteins and small drug molecules. Owing to recent advances, they can be engineered in vitro in both bulk and nanoparticle formats with applications including drug delivery, membrane bioreactors, artificial cells, and biosensors. This review outlines recent advances in cubosome technology enabling their application and provides guidelines for the rational design of new systems for biomedical applications.

JOURNAL ARTICLE

Sang T, Li S, Ting H-K, Stevens MM, Becer CR, Jones JRet al., 2018, Hybrids of Silica/Poly(caprolactone coglycidoxypropyl trimethoxysilane) as Biomaterials, CHEMISTRY OF MATERIALS, Vol: 30, Pages: 3743-3751, ISSN: 0897-4756

JOURNAL ARTICLE

Elsharkawy S, Al-Jawad M, Pantano MF, Tejeda-Montes E, Mehta K, Jamal H, Agarwal S, Shuturminska K, Rice A, Tarakina N, Wilson RM, Bushby AJ, Alonso M, Rodriguez-Cabello JC, Barbieft E, Hernandez ADR, Stevens MM, Pugno NM, Anderson P, Mata Aet al., 2018, Protein disorder-order interplay to guide the growth of hierarchical mineralized structures, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Kapnisi M, Mansfield C, Marijon C, Guex AG, Perbellini F, Bardi I, Humphrey EJ, Puetzer JL, Mawad D, Koutsogeorgis DC, Stuckey DJ, Terracciano CM, Harding SE, Stevens MMet al., 2018, Auxetic Cardiac Patches with Tunable Mechanical and Conductive Properties toward Treating Myocardial Infarction, ADVANCED FUNCTIONAL MATERIALS, Vol: 28, ISSN: 1616-301X

JOURNAL ARTICLE

Spicer CD, Jumeaux C, Gupta B, Stevens MMet al., 2018, Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications, CHEMICAL SOCIETY REVIEWS, Vol: 47, Pages: 3574-3620, ISSN: 0306-0012

JOURNAL ARTICLE

Reznikov N, Bilton M, Lari L, Stevens MM, Kroger Ret al., 2018, Fractal-like hierarchical organization of bone begins at the nanoscale, SCIENCE, Vol: 360, Pages: 507-+, ISSN: 0036-8075

JOURNAL ARTICLE

Armstrong JPK, Stevens MM, 2018, Strategic design of extracellular vesicle drug delivery systems, ADVANCED DRUG DELIVERY REVIEWS, Vol: 130, Pages: 12-16, ISSN: 0169-409X

JOURNAL ARTICLE

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