Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

340 results found

Jumeaux C, Kim E, Howes PD, Kim H, Chandrawati R, Stevens MMet al., 2018, Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion, Nanoscale Advances, Vol: 1, Pages: 532-536

<p>We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay.</p>

JOURNAL ARTICLE

Ghouse S, Reznikov N, Boughton OR, Babu S, Ng KCG, Blunn G, Cobb JP, Stevens MM, Jeffers JRTet al., 2019, The design and in vivo testing of a locally stiffness-matched porous scaffold, Applied Materials Today, Vol: 15, Pages: 377-388

© 2019 The Authors An increasing volume of work supports utilising the mechanobiology of bone for bone ingrowth into a porous scaffold. However, typically during in vivo testing of implants, the mechanical properties of the bone being replaced are not quantified. Consequently there remains inconsistencies in the literature regarding ‘optimum’ pore size and porosity for bone ingrowth. It is also difficult to compare ingrowth results between studies and to translate in vivo animal testing to human subjects without understanding the mechanical environment. This study presents a clinically applicable approach to determining local bone mechanical properties and design of a scaffold with similar properties. The performance of the scaffold was investigated in vivo in an ovine model. The density, modulus and strength of trabecular bone from the medial femoral condyle from ovine bones was characterised and power-law relationships were established. A porous titanium scaffold, intended to maintain bone mechanical homeostasis, was additively manufactured and implanted into the medial femoral condyle of 6 ewes. The stiffness of the scaffold varied throughout the heterogeneous structure and matched the stiffness variation of bone at the surgical site. Bone ingrowth into the scaffold was 10.73 ± 2.97% after 6 weeks. Fine woven bone, in the interior of the scaffold, and intense formations of more developed woven bone overlaid with lamellar bone at the implant periphery were observed. The workflow presented will allow future in vivo testing to test specific bone strains on bone ingrowth in response to a scaffold and allow for better translation from in vivo testing to commercial implants.

JOURNAL ARTICLE

Nele V, Holme MN, Kauscher U, Thomas MR, Doutch JJ, Stevens MMet al., 2019, Effect of Formulation Method, Lipid Composition, and PEGylation on Vesicle Lamellarity: A Small-Angle Neutron Scattering Study., Langmuir

Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of lipid composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the lipid composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phospholipids 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC) via film hydration followed by (i) agitation on a shaker or (ii) freeze-thawing, or (iii) the reverse-phase evaporation vesicle method. After extrusion, up to half of the total lipid content is still assembled into multilamellar structures. However, we achieved unilamellar vesicle populations when as little as 0.1 mol % PEG-modified lipid was included in the vesicle formulation. Interestingly, DPPC with 5 mol % PEGylated lipid produces a combination of cylindrical micelles and vesicles. In conclusion, our results provide important insights into the effect of the formulation method and lipid composition on producing liposomes with a defined membrane structure.

JOURNAL ARTICLE

Paxton NC, Ren J, Ainsworth MJ, Solanki AK, Jones JR, Allenby MC, Stevens MM, Woodruff MAet al., 2019, Rheological Characterization of Biomaterials Directs Additive Manufacturing of Strontium-Substituted Bioactive Glass/Polycaprolactone Microfibers., Macromol Rapid Commun

Additive manufacturing via melt electrowriting (MEW) can create ordered microfiber scaffolds relevant for bone tissue engineering; however, there remain limitations in the adoption of new printing materials, especially in MEW of biomaterials. For example, while promising composite formulations of polycaprolactone with strontium-substituted bioactive glass have been processed into large or disordered fibres, from what is known, biologically-relevant concentrations (>10 wt%) have never been printed into ordered microfibers using MEW. In this study, rheological characterization is used in combination with a predictive mathematical model to optimize biomaterial formulations and MEW conditions required to extrude various PCL and PCL/SrBG biomaterials to create ordered scaffolds. Previously, MEW printing of PCL/SrBG composites with 33 wt% glass required unachievable extrusion pressures. The composite formulation is modified using an evaporable solvent to reduce viscosity 100-fold to fall within the predicted MEW pressure, temperature, and voltage tolerances, which enabled printing. This study reports the first fabrication of reproducible, ordered high-content bioactive glass microfiber scaffolds by applying predictive modeling.

JOURNAL ARTICLE

Li C, Ouyang L, Pence IJ, Moore AC, Lin Y, Winter CW, Armstrong JPK, Stevens MMet al., 2019, Buoyancy-Driven Gradients for Biomaterial Fabrication and Tissue Engineering., Adv Mater, Vol: 31

The controlled fabrication of gradient materials is becoming increasingly important as the next generation of tissue engineering seeks to produce inhomogeneous constructs with physiological complexity. Current strategies for fabricating gradient materials can require highly specialized materials or equipment and cannot be generally applied to the wide range of systems used for tissue engineering. Here, the fundamental physical principle of buoyancy is exploited as a generalized approach for generating materials bearing well-defined compositional, mechanical, or biochemical gradients. Gradient formation is demonstrated across a range of different materials (e.g., polymers and hydrogels) and cargos (e.g., liposomes, nanoparticles, extracellular vesicles, macromolecules, and small molecules). As well as providing versatility, this buoyancy-driven gradient approach also offers speed (<1 min) and simplicity (a single injection) using standard laboratory apparatus. Moreover, this technique is readily applied to a major target in complex tissue engineering: the osteochondral interface. A bone morphogenetic protein 2 gradient, presented across a gelatin methacryloyl hydrogel laden with human mesenchymal stem cells, is used to locally stimulate osteogenesis and mineralization in order to produce integrated osteochondral tissue constructs. The versatility and accessibility of this fabrication platform should ensure widespread applicability and provide opportunities to generate other gradient materials or interfacial tissues.

JOURNAL ARTICLE

Autefage H, Allen F, Tang HM, Kallepitis C, Gentleman E, Reznikov N, Nitiputri K, Nommeots-Nomm A, Young G, Lee PD, Pierce BF, Wagermaier W, Fratzl P, Goodship A, Jones JR, Blunn G, Stevens Met al., Multiscale analyses reveal native-like lamellar bone repair and near perfect bone-contact with porous strontium-loaded bioactive glass, Biomaterials, ISSN: 0142-9612

JOURNAL ARTICLE

Gopal S, Chiappini C, Penders J, Leonardo V, Seong H, Rothery S, Korchev Y, Shevchuk A, Stevens MMet al., 2019, Porous Silicon Nanoneedles Modulate Endocytosis to Deliver Biological Payloads, ADVANCED MATERIALS, Vol: 31, ISSN: 0935-9648

JOURNAL ARTICLE

Barriga HMG, Holme MN, Stevens MM, 2019, Cubosomes: The Next Generation of Smart Lipid Nanoparticles?, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 58, Pages: 2958-2978, ISSN: 1433-7851

JOURNAL ARTICLE

Kim E, Agarwal S, Kim N, Hage FS, Leonardo V, Gelmi A, Stevens MMet al., 2019, Bioinspired Fabrication of DNA-Inorganic Hybrid Composites Using Synthetic DNA, ACS NANO, Vol: 13, Pages: 2888-2900, ISSN: 1936-0851

JOURNAL ARTICLE

Hansel CS, Crowder SW, Cooper S, Gopal S, Pardelha da Cruz MJ, Martins LDO, Keller D, Rothery S, Becce M, Cass AEG, Bakal C, Chiappini C, Stevens MMet al., 2019, Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery, ACS NANO, Vol: 13, Pages: 2913-2926, ISSN: 1936-0851

JOURNAL ARTICLE

Wood CS, Thomas MR, Budd J, Mashamba-Thompson TP, Herbst K, Pillay D, Peeling RW, Johnson AM, McKendry RA, Stevens MMet al., 2019, Taking connected mobile-health diagnostics of infectious diseases to the field, NATURE, Vol: 566, Pages: 467-474, ISSN: 0028-0836

JOURNAL ARTICLE

Armstrong J, Stevens M, 2019, Emerging Technologies for Tissue Engineering: From Gene Editing to Personalized Medicine, Tissue Engineering Part A, ISSN: 1937-3341

JOURNAL ARTICLE

Armstrong JPK, Maynard SA, Pence IJ, Franklin AC, Drinkwater BW, Stevens MMet al., 2019, Spatiotemporal quantification of acoustic cell patterning using Voronoi tessellation, LAB ON A CHIP, Vol: 19, Pages: 562-573, ISSN: 1473-0197

JOURNAL ARTICLE

Reznikov N, Boughton OR, Ghouse S, Weston AE, Collinson L, Blunn GW, Jeffers JRT, Cobb JP, Stevens MMet al., 2019, Individual response variations in scaffold-guided bone regeneration are determined by independent strain- and injury-induced mechanisms, BIOMATERIALS, Vol: 194, Pages: 183-194, ISSN: 0142-9612

JOURNAL ARTICLE

Lin Y, Penna M, Thomas MR, Wojciechowski JP, Leonardo V, Wang Y, Pashuck ET, Yarovsky I, Stevens MMet al., 2019, Residue-Specific Solvation-Directed Thermodynamic and Kinetic Control over Peptide Self-Assembly with 1D/2D Structure Selection, ACS NANO, Vol: 13, Pages: 1900-1909, ISSN: 1936-0851

JOURNAL ARTICLE

Lin Y, Mazo MM, Skaalure SC, Thomas MR, Schultz SR, Stevens MMet al., 2019, Activatable cell-biomaterial interfacing with photo-caged peptides, CHEMICAL SCIENCE, Vol: 10, Pages: 1158-1167, ISSN: 2041-6520

JOURNAL ARTICLE

Kauscher U, Holme MN, Björnmalm M, Stevens MMet al., 2019, Physical stimuli-responsive vesicles in drug delivery: Beyond liposomes and polymersomes., Adv Drug Deliv Rev, Vol: 138, Pages: 259-275

Over the past few decades, a range of vesicle-based drug delivery systems have entered clinical practice and several others are in various stages of clinical translation. While most of these vesicle constructs are lipid-based (liposomes), or polymer-based (polymersomes), recently new classes of vesicles have emerged that defy easy classification. Examples include assemblies with small molecule amphiphiles, biologically derived membranes, hybrid vesicles with two or more classes of amphiphiles, or more complex hierarchical structures such as vesicles incorporating gas bubbles or nanoparticulates in the lumen or membrane. In this review, we explore these recent advances and emerging trends at the edge and just beyond the research fields of conventional liposomes and polymersomes. A focus of this review is the distinct behaviors observed for these classes of vesicles when exposed to physical stimuli - such as ultrasound, heat, light and mechanical triggers - and we discuss the resulting potential for new types of drug delivery, with a special emphasis on current challenges and opportunities.

JOURNAL ARTICLE

Ferrini A, Stevens MM, Sattler S, Rosenthal Net al., 2019, Toward Regeneration of the Heart: Bioengineering Strategies for Immunomodulation., Front Cardiovasc Med, Vol: 6, ISSN: 2297-055X

Myocardial Infarction (MI) is the most common cardiovascular disease. An average-sized MI causes the loss of up to 1 billion cardiomyocytes and the adult heart lacks the capacity to replace them. Although post-MI treatment has dramatically improved survival rates over the last few decades, more than 20% of patients affected by MI will subsequently develop heart failure (HF), an incurable condition where the contracting myocardium is transformed into an akinetic, fibrotic scar, unable to meet the body's need for blood supply. Excessive inflammation and persistent immune auto-reactivity have been suggested to contribute to post-MI tissue damage and exacerbate HF development. Two newly emerging fields of biomedical research, immunomodulatory therapies and cardiac bioengineering, provide potential options to target the causative mechanisms underlying HF development. Combining these two fields to develop biomaterials for delivery of immunomodulatory bioactive molecules holds great promise for HF therapy. Specifically, minimally invasive delivery of injectable hydrogels, loaded with bioactive factors with angiogenic, proliferative, anti-apoptotic and immunomodulatory functions, is a promising route for influencing the cascade of immune events post-MI, preventing adverse left ventricular remodeling, and offering protection from early inflammation to fibrosis. Here we provide an updated overview on the main injectable hydrogel systems and bioactive factors that have been tested in animal models with promising results and discuss the challenges to be addressed for accelerating the development of these novel therapeutic strategies.

JOURNAL ARTICLE

Lin Y, Charchar P, Christofferson AJ, Thomas MR, Todorova N, Mazo MM, Chen Q, Doutch J, Richardson R, Yarovs I, Stevens MMet al., 2018, Surface Dynamics and Ligand-Core Interactions of Quantum Sized Photoluminescent Gold Nanoclusters, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 140, Pages: 18217-18226, ISSN: 0002-7863

JOURNAL ARTICLE

Pujari-Palmer M, Guo H, Wenner D, Autefage H, Spicer CD, Stevens MM, Omar O, Thomsen P, Eden M, Insley G, Procter P, Engqvist Het al., 2018, A Novel Class of Injectable Bioceramics That Glue Tissues and Biomaterials, MATERIALS, Vol: 11, ISSN: 1996-1944

JOURNAL ARTICLE

Armstrong JPK, Puetzer JL, Serio A, Guex AG, Kapnisi M, Breant A, Zong Y, Assal V, Skaalure SC, King O, Murty T, Meinert C, Franklin AC, Bassindale PG, Nichols MK, Terracciano CM, Hutmacher DW, Drinkwater BW, Klein TJ, Perriman AW, Stevens MMet al., 2018, Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning, ADVANCED MATERIALS, Vol: 30, ISSN: 0935-9648

JOURNAL ARTICLE

Penders J, Pence IJ, Horgan CC, Bergholt MS, Wood CS, Najer A, Kauscher U, Nagelkerke A, Stevens MMet al., 2018, Single Particle Automated Raman Trapping Analysis, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Gray ER, Bain R, Varsaneux O, Peeling RW, Stevens MM, McKendry RAet al., 2018, p24 revisited: a landscape review of antigen detection for early HIV diagnosis, AIDS, Vol: 32, Pages: 2089-2102, ISSN: 0269-9370

JOURNAL ARTICLE

Amdursky N, Mazo MM, Thomas MR, Humphrey EJ, Puetzer JL, St-Pierre J-P, Skaalure SC, Richardson RM, Terracciano CM, Stevens MMet al., 2018, Elastic serum-albumin based hydrogels: mechanism of formation and application in cardiac tissue engineering, JOURNAL OF MATERIALS CHEMISTRY B, Vol: 6, Pages: 5604-5612, ISSN: 2050-750X

JOURNAL ARTICLE

Sigmundsson K, Ojala JRM, Ohman MK, Osterholm A-M, Moreno-Moral A, Domogatskaya A, Chong LY, Sun Y, Chai X, Steele JAM, George B, Patarroyo M, Nilsson A-S, Rodin S, Ghosh S, Stevens MM, Petretto E, Tryggvason Ket al., 2018, Culturing functional pancreatic islets on alpha 5-Iaminins and curative transplantation , to diabetic mice, MATRIX BIOLOGY, Vol: 70, Pages: 5-19, ISSN: 0945-053X

JOURNAL ARTICLE

Faria M, Bjornmalm M, Thurecht KJ, Kent SJ, Parton RG, Kavallaris M, Johnston APR, Gooding JJ, Corrie SR, Boyd BJ, Thordarson P, Whittaker AK, Stevens MM, Prestidge CA, Porter CJH, Parak WJ, Davis TP, Crampin EJ, Caruso Fet al., 2018, Minimum information reporting in bio-nano experimental literature, NATURE NANOTECHNOLOGY, Vol: 13, Pages: 777-785, ISSN: 1748-3387

JOURNAL ARTICLE

Tallia F, Russo L, Li S, Orrin ALH, Shi X, Chen S, Steele JAM, Meille S, Chevalier J, Lee PD, Stevens MM, Cipolla L, Jones JRet al., 2018, Bouncing and 3D printable hybrids with self-healing properties, MATERIALS HORIZONS, Vol: 5, Pages: 849-860, ISSN: 2051-6347

JOURNAL ARTICLE

Li C, Armstrong JPK, Pence IJ, Kit-Anan W, Puetzer JL, Carreira SC, Moore AC, Stevens MMet al., 2018, Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering, BIOMATERIALS, Vol: 176, Pages: 24-33, ISSN: 0142-9612

JOURNAL ARTICLE

Wang Y, Howes PD, Kim E, Spicer CD, Thomas MR, Lin Y, Crowder SW, Pence IJ, Stevens MMet al., 2018, Duplex-Specific Nuclease-Amplified Detection of MicroRNA Using Compact Quantum Dot-DNA Conjugates, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 28290-28300, ISSN: 1944-8244

JOURNAL ARTICLE

Spicer CD, Pashuck ET, Stevens MM, 2018, Achieving Controlled Biomolecule-Biomaterial Conjugation, CHEMICAL REVIEWS, Vol: 118, Pages: 7702-7743, ISSN: 0009-2665

JOURNAL ARTICLE

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