317 results found
Albro MB, Bergholt MS, St-Pierre JP, et al., 2018, Raman spectroscopic imaging for quantification of depth-dependent and local heterogeneities in native and engineered cartilage, NPJ REGENERATIVE MEDICINE, Vol: 3, ISSN: 2057-3995
Armstrong JPK, Stevens MM, 2018, Strategic design of extracellular vesicle drug delivery systems., Adv Drug Deliv Rev
Extracellular vesicles (EVs), sub-micron vectors used in intercellular communication, have demonstrated great promise as natural drug delivery systems. Recent reports have detailed impressive in vivo results from the administration of EVs pre-loaded with therapeutic cargo, including small molecules, nanoparticles, proteins and oligonucleotides. These results have sparked intensive research interest across a huge range of disease models. There are, however, enduring limitations that have restricted widespread clinical and pharmaceutical adoption. In this perspective, we discuss these practical and biological concerns, critically compare the relative merit of EVs and synthetic drug delivery systems, and highlight the need for a more comprehensive understanding of in vivo transport and delivery. Within this framework, we seek to establish key areas in which EVs can gain a competitive advantage in order to provide the tangible added value required for widespread translation.
Barriga HMG, Holme MN, Stevens MM, 2018, Cubosomes; the next generation of smart lipid nanoparticles?, Angew Chem Int Ed Engl
Cubosomes are highly stable nanoparticles formed from the lipid cubic phase and stabilized by a polymer based outer corona. Lipid cubic phases consist of a single lipid bilayer which forms a continuous periodic membrane lattice structure with pores formed by two interwoven water channels. Cubosome composition can be tuned to engineer pore sizes or include bioactive lipids, the polymer outer corona can be used for targeting and they are highly stable under physiological conditions. The structure provides a significantly higher membrane surface area for loading of membrane proteins and small drug molecules than liposomes. Due to recent advances, they can be engineered in vitro in both bulk and nanoparticle formats with applications including drug delivery, membrane bioreactors, artificial cells and biosensors. This review outlines recent advances in cubosome technology enabling their application and provides guidelines for the rational design of new systems for biomedical applications.
Bergholt MS, Serio A, McKenzie JS, et al., 2018, Correlated Heterospectral Lipidomics for Biomolecular Profiling of Remyelination in Multiple Sclerosis, ACS CENTRAL SCIENCE, Vol: 4, Pages: 39-51, ISSN: 2374-7943
Brangel P, Sobarzo A, Parolo C, et al., 2018, A Serological Point-of-Care Test for the Detection of IgG Antibodies against Ebola Virus in Human Survivors, ACS NANO, Vol: 12, Pages: 63-73, ISSN: 1936-0851
Elsharkawy S, Al-Jawad M, Pantano MF, et al., 2018, Protein disorder-order interplay to guide the growth of hierarchical mineralized structures, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723
Fuhrmann G, Chandrawati R, Parmar PA, et al., 2018, Engineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy, ADVANCED MATERIALS, Vol: 30, ISSN: 0935-9648
Holme MN, Rana S, Barriga HMG, et al., 2018, A Robust Liposomal Platform for Direct Colorimetric Detection of Sphingomyelinase Enzyme and Inhibitors., ACS Nano
The enzyme sphingomyelinase (SMase) is an important biomarker for several diseases such as Niemann Pick's, atherosclerosis, multiple sclerosis, and HIV. We present a two-component colorimetric SMase activity assay that is more sensitive and much faster than currently available commercial assays. Herein, SMase-triggered release of cysteine from a sphingomyelin (SM)-based liposome formulation with 60 mol % cholesterol causes gold nanoparticle (AuNP) aggregation, enabling colorimetric detection of SMase activities as low as 0.02 mU/mL, corresponding to 1.4 pM concentration. While the lipid composition offers a stable, nonleaky liposome platform with minimal background signal, high specificity toward SMase avoids cross-reactivity of other similar phospholipases. Notably, use of an SM-based liposome formulation accurately mimics the natural in vivo substrate: the cell membrane. We studied the physical rearrangement process of the lipid membrane during SMase-mediated hydrolysis of SM to ceramide using small- and wide-angle X-ray scattering. A change in lipid phase from a liquid to gel state bilayer with increasing concentration of ceramide accounts for the observed increase in membrane permeability and consequent release of encapsulated cysteine. We further demonstrated the effectiveness of the sensor in colorimetric screening of small-molecule drug candidates, paving the way for the identification of novel SMase inhibitors in minutes. Taken together, the simplicity, speed, sensitivity, and naked-eye readout of this assay offer huge potential in point-of-care diagnostics and high-throughput drug screening.
Hsu C-C, Serio A, Arndursky N, et al., 2018, Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 5305-5317, ISSN: 1944-8244
Jumeaux C, Wahlsten O, Block S, et al., 2018, MicroRNA Detection by DNA-Mediated Liposome Fusion, CHEMBIOCHEM, Vol: 19, Pages: 434-438, ISSN: 1439-4227
Kapnisi M, Mansfield C, Marijon C, et al., 2018, Auxetic Cardiac Patches with Tunable Mechanical and Conductive Properties toward Treating Myocardial Infarction, ADVANCED FUNCTIONAL MATERIALS, Vol: 28, ISSN: 1616-301X
Keane TJ, Horejs C-M, Stevens MM, 2018, Scarring vs. functional healing: Matrix-based strategies to regulate tissue repair, ADVANCED DRUG DELIVERY REVIEWS, Vol: 129, Pages: 407-419, ISSN: 0169-409X
Li C, Armstrong JPK, Pence IJ, et al., 2018, Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering, BIOMATERIALS, Vol: 176, Pages: 24-33, ISSN: 0142-9612
Littmann E, Autefage H, Solanki AK, et al., 2018, Cobalt-containing bioactive glasses reduce human mesenchymal stem cell chondrogenic differentiation despite HIF-1 alpha stabilisation, JOURNAL OF THE EUROPEAN CERAMIC SOCIETY, Vol: 38, Pages: 877-886, ISSN: 0955-2219
Loynachan CN, Thomas MR, Gray ER, et al., 2018, Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range, ACS NANO, Vol: 12, Pages: 279-288, ISSN: 1936-0851
Milner PE, Parkes M, Puetzer JL, et al., 2018, A low friction, biphasic and boundary lubricating hydrogel for cartilage replacement, ACTA BIOMATERIALIA, Vol: 65, Pages: 102-111, ISSN: 1742-7061
Reznikov N, Bilton M, Lari L, et al., 2018, Fractal-like hierarchical organization of bone begins at the nanoscale, SCIENCE, Vol: 360, Pages: 507-+, ISSN: 0036-8075
Rizzo R, Alvaro M, Danz N, et al., 2018, Bloch surface wave enhanced biosensor for the direct detection of angiopoietin-2 tumor biomarker in human plasma, Biomedical Optics Express, Vol: 9, Pages: 529-542, ISSN: 2156-7085
Quantitative detection of angiogenic biomarkers provides a powerful tool to diagnose cancers in early stages and to follow its progression during therapy. Conventional tests require trained personnel, dedicated laboratory equipment and are generally time-consuming. Herein, we propose our developed biosensing platform as a useful tool for a rapid determination of Angiopoietin-2 biomarker directly from patient plasma within 30 minutes, without any sample preparation or dilution. Bloch surface waves supported by one dimensional photonic crystal are exploited to enhance and redirect the fluorescence arising from a sandwich immunoassay that involves Angiopoietin-2. The sensing units consist of disposable and low-cost plastic biochips coated with the photonic crystal. The biosensing platform is demonstrated to detect Angiopoietin-2 in plasma samples at the clinically relevant concentration of 6 ng/mL, with an estimated limit of detection of approximately 1 ng/mL. This is the first Bloch surface wave based assay capable of detecting relevant concentrations of an angiogenic factor in plasma samples. The results obtained by the developed biosensing platform are in close agreement with enzyme-linked immunosorbent assays, demonstrating a good accuracy, and their repeatability showed acceptable relative variations.
Sang T, Li S, Ting H-K, et al., 2018, Hybrids of Silica/Poly(caprolactone coglycidoxypropyl trimethoxysilane) as Biomaterials, CHEMISTRY OF MATERIALS, Vol: 30, Pages: 3743-3751, ISSN: 0897-4756
Sigmundsson K, Ojala JRM, Öhman MK, et al., 2018, Culturing functional pancreatic islets on α5-laminins and curative transplantation to diabetic mice., Matrix Biol, Vol: 70, Pages: 5-19
The efficacy of islet transplantation for diabetes treatment suffers from lack of cadaver-derived islets, islet necrosis and long transfer times prior to transplantation. Here, we developed a method for culturing mouse and human islets in vitro on α5-laminins, which are natural components of islet basement membranes. Adhering islets spread to form layers of 1-3 cells in thickness and remained normoxic and functional for at least 7 days in culture. In contrast, spherical islets kept in suspension developed hypoxia and central necrosis within 16 h. Transplantation of 110-150 mouse islets cultured on α5-laminin-coated polydimethylsiloxane membranes for 3-7 days normalized blood glucose already within 3 days in mice with streptozotocin-induced diabetes. RNA-sequencing of isolated and cultured mouse islets provided further evidence for the adhesion and spreading achieved with α5-laminin. Our results suggest that use of such in vitro expanded islets may significantly enhance the efficacy of islet transplantation treatment for diabetes.
Spicer CD, Jumeaux C, Gupta B, et al., 2018, Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications, CHEMICAL SOCIETY REVIEWS, Vol: 47, Pages: 3574-3620, ISSN: 0306-0012
Spicer CD, Pashuck ET, Stevens MM, 2018, Achieving Controlled Biomolecule-Biomaterial Conjugation., Chem Rev
The conjugation of biomolecules can impart materials with the bioactivity necessary to modulate specific cell behaviors. While the biological roles of particular polypeptide, oligonucleotide, and glycan structures have been extensively reviewed, along with the influence of attachment on material structure and function, the key role played by the conjugation strategy in determining activity is often overlooked. In this review, we focus on the chemistry of biomolecule conjugation and provide a comprehensive overview of the key strategies for achieving controlled biomaterial functionalization. No universal method exists to provide optimal attachment, and here we will discuss both the relative advantages and disadvantages of each technique. In doing so, we highlight the importance of carefully considering the impact and suitability of a particular technique during biomaterial design.
Winther AK, Fejerskov B, ter Meer M, et al., 2018, Enzyme Prodrug Therapy Achieves Site-Specific, Personalized Physiological Responses to the Locally Produced Nitric Oxide, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 10741-10751, ISSN: 1944-8244
von Erlach TC, Bertazzo S, Wozniak MA, et al., 2018, Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate, NATURE MATERIALS, Vol: 17, Pages: 237-+, ISSN: 1476-1122
Amdursky N, Rashid MH, Stevens MM, et al., 2017, Exploring the binding sites and proton diffusion on insulin amyloid fibril surfaces by naphthol-based photoacid fluorescence and molecular simulations, SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322
Amdursky N, Wang X, Meredith P, et al., 2017, Electron Hopping Across Hemin-Doped Serum Albumin Mats on Centimeter-Length Scales, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648
Armstrong JPK, Holme MN, Stevens MM, 2017, Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics, ACS NANO, Vol: 11, Pages: 69-83, ISSN: 1936-0851
Bergholt MS, Albro MB, Stevens MM, 2017, Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy, BIOMATERIALS, Vol: 140, Pages: 128-137, ISSN: 0142-9612
Chandrawati R, Chang JYH, Reina-Torres E, et al., 2017, Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648
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