Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

308 results found

Albro MB, Bergholt MS, St-Pierre JP, Guitart AV, Zlotnick HM, Evita EG, Stevens MMet al., 2018, Raman spectroscopic imaging for quantification of depth-dependent and local heterogeneities in native and engineered cartilage, NPJ REGENERATIVE MEDICINE, Vol: 3, ISSN: 2057-3995

JOURNAL ARTICLE

Bergholt MS, Serio A, McKenzie JS, Boyd A, Soares RF, Tillner J, Chiappini C, Wu V, Dannhorn A, Takats Z, Williams A, Stevens MMet al., 2018, Correlated Heterospectral Lipidomics for Biomolecular Profiling of Remyelination in Multiple Sclerosis, ACS CENTRAL SCIENCE, Vol: 4, Pages: 39-51, ISSN: 2374-7943

JOURNAL ARTICLE

Brangel P, Sobarzo A, Parolo C, Miller BS, Howes PD, Gelkop S, Lutwarna JJ, Dye JM, McKendry RA, Lobel L, Stevens MMet al., 2018, A Serological Point-of-Care Test for the Detection of IgG Antibodies against Ebola Virus in Human Survivors, ACS NANO, Vol: 12, Pages: 63-73, ISSN: 1936-0851

JOURNAL ARTICLE

Elsharkawy S, Al-Jawad M, Pantano MF, Tejeda-Montes E, Mehta K, Jamal H, Agarwal S, Shuturminska K, Rice A, Tarakina N, Wilson RM, Bushby AJ, Alonso M, Rodriguez-Cabello JC, Barbieft E, Hernandez ADR, Stevens MM, Pugno NM, Anderson P, Mata Aet al., 2018, Protein disorder-order interplay to guide the growth of hierarchical mineralized structures, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Fuhrmann G, Chandrawati R, Parmar PA, Keane TJ, Maynard SA, Bertazzo S, Stevens MMet al., 2018, Engineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy, ADVANCED MATERIALS, Vol: 30, ISSN: 0935-9648

JOURNAL ARTICLE

Hsu C-C, Serio A, Arndursky N, Besnard C, Stevens MMet al., 2018, Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 5305-5317, ISSN: 1944-8244

JOURNAL ARTICLE

Jumeaux C, Wahlsten O, Block S, Kim E, Chandrawati R, Howes PD, Hook F, Stevens MMet al., 2018, MicroRNA Detection by DNA-Mediated Liposome Fusion, CHEMBIOCHEM, Vol: 19, Pages: 434-438, ISSN: 1439-4227

JOURNAL ARTICLE

Kapnisi M, Mansfield C, Marijon C, Guex AG, Perbellini F, Bardi I, Humphrey EJ, Puetzer JL, Mawad D, Koutsogeorgis DC, Stuckey DJ, Terracciano CM, Harding SE, Stevens MMet al., 2018, Auxetic Cardiac Patches with Tunable Mechanical and Conductive Properties toward Treating Myocardial Infarction., Adv Funct Mater, Vol: 28, ISSN: 1616-301X

An auxetic conductive cardiac patch (AuxCP) for the treatment of myocardial infarction (MI) is introduced. The auxetic design gives the patch a negative Poisson's ratio, providing it with the ability to conform to the demanding mechanics of the heart. The conductivity allows the patch to interface with electroresponsive tissues such as the heart. Excimer laser microablation is used to micropattern a re-entrant honeycomb (bow-tie) design into a chitosan-polyaniline composite. It is shown that the bow-tie design can produce patches with a wide range in mechanical strength and anisotropy, which can be tuned to match native heart tissue. Further, the auxetic patches are conductive and cytocompatible with murine neonatal cardiomyocytes in vitro. Ex vivo studies demonstrate that the auxetic patches have no detrimental effect on the electrophysiology of both healthy and MI rat hearts and conform better to native heart movements than unpatterned patches of the same material. Finally, the AuxCP applied in a rat MI model results in no detrimental effect on cardiac function and negligible fibrotic response after two weeks in vivo. This approach represents a versatile and robust platform for cardiac biomaterial design and could therefore lead to a promising treatment for MI.

JOURNAL ARTICLE

Keane TJ, Horejs C-M, Stevens MM, 2018, Scarring vs. functional healing: Matrix-based strategies to regulate tissue repair., Adv Drug Deliv Rev

All vertebrates possess mechanisms to restore damaged tissues with outcomes ranging from regeneration to scarring. Unfortunately, the mammalian response to tissue injury most often culminates in scar formation. Accounting for nearly 45% of deaths in the developed world, fibrosis is a process that stands diametrically opposed to functional tissue regeneration. Strategies to improve wound healing outcomes therefore require methods to limit fibrosis. Wound healing is guided by precise spatiotemporal deposition and remodelling of the extracellular matrix (ECM). The ECM, comprising the non-cellular component of tissues, is a signalling depot that is differentially regulated in scarring and regenerative healing. This Review focuses on the importance of the native matrix components during mammalian wound healing alongside a comparison to scar-free healing and then presents an overview of matrix-based strategies that attempt to exploit the role of the ECM to improve wound healing outcomes.

JOURNAL ARTICLE

Li C, Armstrong JP, Pence IJ, Kit-Anan W, Puetzer JL, Correia Carreira S, Moore AC, Stevens MMet al., 2018, Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering., Biomaterials, Vol: 176, Pages: 24-33

In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function. Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs. In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems. Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage. Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes. This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.

JOURNAL ARTICLE

Littmann E, Autefage H, Solanki AK, Kallepitis C, Jones JR, Alini M, Peroglio M, Stevens MMet al., 2018, Cobalt-containing bioactive glasses reduce human mesenchymal stem cell chondrogenic differentiation despite HIF-1 alpha stabilisation, JOURNAL OF THE EUROPEAN CERAMIC SOCIETY, Vol: 38, Pages: 877-886, ISSN: 0955-2219

JOURNAL ARTICLE

Loynachan CN, Thomas MR, Gray ER, Richards DA, Kim J, Miller BS, Brookes JC, Agarwal S, Chudasama V, McKendry RA, Stevens MMet al., 2018, Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range, ACS NANO, Vol: 12, Pages: 279-288, ISSN: 1936-0851

JOURNAL ARTICLE

Milner PE, Parkes M, Puetzer JL, Chapman R, Stevens MM, Cann P, Jeffers JRTet al., 2018, A low friction, biphasic and boundary lubricating hydrogel for cartilage replacement, ACTA BIOMATERIALIA, Vol: 65, Pages: 102-111, ISSN: 1742-7061

JOURNAL ARTICLE

Reznikov N, Bilton M, Lari L, Stevens MM, Kroger Ret al., 2018, Fractal-like hierarchical organization of bone begins at the nanoscale, SCIENCE, Vol: 360, Pages: 507-+, ISSN: 0036-8075

JOURNAL ARTICLE

Rizzo R, Alvaro M, Danz N, Napione L, Descrovi E, Schmieder S, Sinibaldi A, Rana S, Chandrawati R, Munzert P, Schubert T, Maillart E, Anopchenko A, Rivolo P, Mascioletti A, Förster E, Sonntag F, Stevens MM, Bussolino F, Michelotti Fet al., 2018, Bloch surface wave enhanced biosensor for the direct detection of angiopoietin-2 tumor biomarker in human plasma, Biomedical Optics Express, Vol: 9, Pages: 529-542, ISSN: 2156-7085

Quantitative detection of angiogenic biomarkers provides a powerful tool to diagnose cancers in early stages and to follow its progression during therapy. Conventional tests require trained personnel, dedicated laboratory equipment and are generally time-consuming. Herein, we propose our developed biosensing platform as a useful tool for a rapid determination of Angiopoietin-2 biomarker directly from patient plasma within 30 minutes, without any sample preparation or dilution. Bloch surface waves supported by one dimensional photonic crystal are exploited to enhance and redirect the fluorescence arising from a sandwich immunoassay that involves Angiopoietin-2. The sensing units consist of disposable and low-cost plastic biochips coated with the photonic crystal. The biosensing platform is demonstrated to detect Angiopoietin-2 in plasma samples at the clinically relevant concentration of 6 ng/mL, with an estimated limit of detection of approximately 1 ng/mL. This is the first Bloch surface wave based assay capable of detecting relevant concentrations of an angiogenic factor in plasma samples. The results obtained by the developed biosensing platform are in close agreement with enzyme-linked immunosorbent assays, demonstrating a good accuracy, and their repeatability showed acceptable relative variations.

JOURNAL ARTICLE

Sang T, Li S, Ting HK, Stevens MM, Becer CR, Jones JRet al., 2018, Hybrids of silica/poly (caprolactone co-glycidoxypropyl trimethoxysilane) as biomaterials, Chemistry of Materials, ISSN: 0897-4756

© 2018 American Chemical Society. Bioactive glasses stimulate bone regeneration but are brittle. Biomaterials are needed that share load with bone, promote bone regeneration and biodegrade at controlled rates. Sol-gel hybrids can achieve this through their intimate inorganic and organic co-networks, depending on the organic polymer used. Polycaprolactone degrades slowly but lacks functional groups for the critical step of covalent coupling to the silica co-network. Here, we synthesised a novel copolymer of caprolactone and glycidoxypropyl trimethoxysilane through one-pot ring opening polymerization (ROP). Hybrids with different organic content were fabricated using such a copolymer for the first time. The copolymer can directly bond to a silica network due its trimethoxysilane groups, which can hydrolyse, leaving silanol groups that undergo polycondensation with silanol groups of the silica network. Number of repeating units of caprolactone and glycidoxypropyl trimethoxysilane functional groups were controlled via ROP. The mechanical properties of the hybrids were tuned by weight percent and the number of repeating units of caprolactone independently, producing a homogeneous material with high strength (64 MPa) and strain to failure (20%) that deformed in a unique linear elastic manner until failure. MC3T3-E1 pre-osteoblast cells adhered to the hybrids. Introducing such a copolymer created a new way to fabricate covalently bonded polycaprolactone/silica hybrids for future bone repair.

JOURNAL ARTICLE

Sigmundsson K, Ojala JRM, Öhman MK, Österholm A-M, Moreno-Moral A, Domogatskaya A, Chong LY, Sun Y, Chai X, Steele JAM, George B, Patarroyo M, Nilsson A-S, Rodin S, Ghosh S, Stevens MM, Petretto E, Tryggvason Ket al., 2018, Culturing functional pancreatic islets on α5-laminins and curative transplantation to diabetic mice., Matrix Biol

The efficacy of islet transplantation for diabetes treatment suffers from lack of cadaver-derived islets, islet necrosis and long transfer times prior to transplantation. Here, we developed a method for culturing mouse and human islets in vitro on α5-laminins, which are natural components of islet basement membranes. Adhering islets spread to form layers of 1-3 cells in thickness and remained normoxic and functional for at least 7 days in culture. In contrast, spherical islets kept in suspension developed hypoxia and central necrosis within 16 h. Transplantation of 110-150 mouse islets cultured on α5-laminin-coated polydimethylsiloxane membranes for 3-7 days normalized blood glucose already within 3 days in mice with streptozotocin-induced diabetes. RNA-sequencing of isolated and cultured mouse islets provided further evidence for the adhesion and spreading achieved with α5-laminin. Our results suggest that use of such in vitro expanded islets may significantly enhance the efficacy of islet transplantation treatment for diabetes.

JOURNAL ARTICLE

Spicer CD, Jumeaux C, Gupta B, Stevens MMet al., 2018, Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications, CHEMICAL SOCIETY REVIEWS, Vol: 47, Pages: 3574-3620, ISSN: 0306-0012

JOURNAL ARTICLE

Winther AK, Fejerskov B, ter Meer M, Jensen NBS, Dillison R, Schaffer JE, Chandrawati R, Stevens MM, Kool LJS, Simonsen U, Zelikin ANet al., 2018, Enzyme Prodrug Therapy Achieves Site-Specific, Personalized Physiological Responses to the Locally Produced Nitric Oxide, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 10741-10751, ISSN: 1944-8244

JOURNAL ARTICLE

von Erlach TC, Bertazzo S, Wozniak MA, Horejs C-M, Maynard SA, Attwood S, Robinson BK, Autefage H, Kallepitis C, Hernandez ADR, Chen CS, Goldoni S, Stevens MMet al., 2018, Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate, NATURE MATERIALS, Vol: 17, Pages: 237-+, ISSN: 1476-1122

JOURNAL ARTICLE

Amdursky N, Wang X, Meredith P, Riley DJ, Payne DJ, Bradley DDC, Stevens MMet al., 2017, Electron Hopping Across Hemin-Doped Serum Albumin Mats on Centimeter-Length Scales, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648

JOURNAL ARTICLE

Armstrong JPK, Holme MN, Stevens MM, 2017, Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics, ACS NANO, Vol: 11, Pages: 69-83, ISSN: 1936-0851

JOURNAL ARTICLE

Bergholt MS, Albro MB, Stevens MM, 2017, Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy, BIOMATERIALS, Vol: 140, Pages: 128-137, ISSN: 0142-9612

JOURNAL ARTICLE

Chan WCW, Chhowalla M, Glotzer S, Gogotsi Y, Hafner JH, Hammond PT, Hersam MC, Javey A, Kagan CR, Kataoka K, Khademhosseini A, Kotov NA, Lee S-T, Li Y, Moehwald H, Mulvaney P, Nel AE, Nordlander PJ, Parak WJ, Penner RM, Rogach AL, Schaak RE, Stevens MM, Wee ATS, Willson CG, Fernandez LEE, Weiss PSet al., 2017, Our First and Next Decades at ACS Nano, Publisher: AMER CHEMICAL SOC

OTHER

Chandrawati R, Chang JYH, Reina-Torres E, Jumeaux C, Sherwood JM, Stamer WD, Zelikin AN, Overby DR, Stevens MMet al., 2017, Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648

JOURNAL ARTICLE

Chandrawati R, Olesen MTJ, Marini TCC, Bisra G, Guex AG, de Oliveira MG, Zelikin AN, Stevens MMet al., 2017, Enzyme Prodrug Therapy Engineered into Electrospun Fibers with Embedded Liposomes for Controlled, Localized Synthesis of Therapeutics, ADVANCED HEALTHCARE MATERIALS, Vol: 6, ISSN: 2192-2640

JOURNAL ARTICLE

Chang JYH, Chow LW, Dismuke WM, Ethier CR, Stevens MM, Stamer WD, Overby DRet al., 2017, Peptide-Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite-Mediated Nitration, ADVANCED HEALTHCARE MATERIALS, Vol: 6, ISSN: 2192-2640

JOURNAL ARTICLE

Chow A, Stuckey DJ, Kidher E, Rocco M, Jabbour RJ, Mansfield CA, Darzi A, Harding SE, Stevens MM, Athanasiou Tet al., 2017, Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Encapsulating Bioactive Hydrogels Improve Rat Heart Function Post Myocardial Infarction, STEM CELL REPORTS, Vol: 9, Pages: 1415-1422, ISSN: 2213-6711

JOURNAL ARTICLE

Chung JJ, Fujita Y, Li S, Stevens MM, Kasuga T, Georgiou TK, Jones JRet al., 2017, Biodegradable inorganic-organic hybrids of methacrylate star polymers for bone regeneration, ACTA BIOMATERIALIA, Vol: 54, Pages: 411-418, ISSN: 1742-7061

JOURNAL ARTICLE

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