Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

241 results found

Amdursky N, Wang X, Meredith P, Riley DJ, Payne DJ, Bradley D, Stevens MMet al., Electron Hopping Across Hemin-Doped Serum Albumin Mats on Centimetre-Length Scales, Advanced Materials, ISSN: 1521-4095

JOURNAL ARTICLE

Chang JYH, Chow LW, Dismuke WM, Ethier CR, Stevens MM, Stamer WD, Overby Det al., Peptide-Functionalized Fluorescent Particles for in situ Detection of Nitric Oxide via Peroxynitrite-Mediated Nitration, Advanced Healthcare Materials, ISSN: 2192-2640

JOURNAL ARTICLE

Clarke D, Pashuck ET, Bertazzo S, Weaver JVM, Stevens MMet al., Self-Healing Self-Assembled B-sheet Peptide Poly(G-glutamic acid) Hybrid Hydrogels, Journal of the American Chemical Society, ISSN: 1520-5126

JOURNAL ARTICLE

Horejs CM, St-Pierre JP, Ojala JRM, Steele JAM, Barros da Silva P, Rynne-Vidal A, Maynard SA, Hansel CS, Rodriguez-Fernandez C, Mazo MM, You AYF, Wang AJ, von Erlach T, Tryggvason K, Lopez-Cabrera M, Stevens MMet al., Preventing tissue fibrosis by local biomaterials interfacing of specific cryptic extracellular matrix information, Nature Communications, ISSN: 2041-1723

JOURNAL ARTICLE

Soh JH, Lin Y, Thomas MR, Todorova N, Kallepitis C, Ying JY, Yarovsky I, Stevens MMet al., Distinct Bimodal Roles of Aromatic Molecules in Controlling Gold Nanorod Growth for Biosensing, Advanced Functional Materials, ISSN: 1616-3028

JOURNAL ARTICLE

Wang C, Lin Y, Hsu C, Amdursky N, Spicer C, Stevens MMet al., Probing Amylin Fibrillation at an Early Stage via a Tetracysteine-Recognising Fluorophore, Talanta, ISSN: 1873-3573

JOURNAL ARTICLE

Armstrong JPK, Holme MN, Stevens MM, 2017, Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics, ACS NANO, Vol: 11, Pages: 69-83, ISSN: 1936-0851

JOURNAL ARTICLE

Chandrawati R, Chang JYH, Reina-Torres E, Jumeaux C, Sherwood JM, Stamer WD, Zelikin AN, Overby DR, Stevens MMet al., 2017, Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma., Adv Mater, Vol: 29

Nitric oxide (NO) is able to lower intraocular pressure (IOP); however, its therapeutic effects on outflow physiology are location- and dose-dependent. A NO delivery platform that directly targets the resistance-generating region of the conventional outflow pathway and locally liberates a controlled dose of NO is reported. An increase in outflow facility (decrease in IOP) is demonstrated in a mouse model.

JOURNAL ARTICLE

Chung JJ, Fujita Y, Li S, Stevens MM, Kasuga T, Georgiou TK, Jones JRet al., 2017, Biodegradable inorganic-organic hybrids of methacrylate star polymers for bone regeneration., Acta Biomater, Vol: 54, Pages: 411-418

Hybrids that are molecular scale co-networks of organic and inorganic components are promising biomaterials, improving the brittleness of bioactive glass and the strength of polymers. Methacrylate polymers have high potential as the organic source for hybrids since they can be produced, through controlled polymerization, with sophisticated polymer architectures that can bond to silicate networks. Previous studies showed the mechanical properties of hybrids can be modified by polymer architecture and molar mass (MM). However, biodegradability is critical if hybrids are to be used as tissue engineering scaffolds, since the templates must be remodelled by host tissue. Degradation by-products have to either completely biodegrade or be excreted by the kidneys. Enzyme, or bio-degradation is preferred to hydrolysis by water uptake as it is expected to give a more controlled degradation rate. Here, branched and star shaped poly(methyl methacrylate-co-3-(trimethoxysilyl)propyl methacrylate) (poly(MMA-co-TMSPMA)) were synthesized with disulphide based dimethacrylate (DSDMA) as a biodegradable branching agent. Biodegradability was confirmed by exposing the copolymers to glutathione, a tripeptide which is known to cleave disulphide bonds. Cleaved parts of the star polymer from the hybrid system were detected after 2weeks of immersion in glutathione solution, and MM was under threshold of kidney filtration. The presence of the branching agent did not reduce the mechanical properties of the hybrids and bone progenitor cells attached on the hybrids in vitro. Incorporation of the DSDMA branching agent has opened more possibilities to design biodegradable methacrylate polymer based hybrids for regenerative medicine. STATEMENT OF SIGNIFICANCE: Bioactive glasses can regenerate bone but are brittle. Hybrids can overcome this problem as intimate interactions between glass and polymer creates synergetic properties. Implants have previously been made with synthetic polymers that degrade b

JOURNAL ARTICLE

Fiocco L, Li S, Stevens MM, Bernardo E, Jones JRet al., 2017, Biocompatibility and bioactivity of porous polymer-derived Ca-Mg silicate ceramics, ACTA BIOMATERIALIA, Vol: 50, Pages: 56-67, ISSN: 1742-7061

JOURNAL ARTICLE

Higgins SG, Stevens MM, 2017, Extracting the contents of living cells., Science, Vol: 356, Pages: 379-380

JOURNAL ARTICLE

Kallepitis C, Bergholt MS, Mazo MM, Leonardo V, Skaalure SC, Maynard SA, Stevens MMet al., 2017, Quantitative volumetric Raman imaging of three dimensional cell cultures, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723

JOURNAL ARTICLE

Kim E, Howes PD, Crowder SW, Stevens MMet al., 2017, Multi-Amplified Sensing of MicroRNA by a Small DNA Fragment-Driven Enzymatic Cascade Reaction, ACS SENSORS, Vol: 2, Pages: 111-118, ISSN: 2379-3694

JOURNAL ARTICLE

Kim E, Zwi-Dantsis L, Reznikov N, Hansel CS, Agarwal S, Stevens MMet al., 2017, One-Pot Synthesis of Multiple Protein-Encapsulated DNA Flowers and Their Application in Intracellular Protein Delivery., Adv Mater

Inspired by biological systems, many biomimetic methods suggest fabrication of functional materials with unique physicochemical properties. Such methods frequently generate organic-inorganic composites that feature highly ordered hierarchical structures with intriguing properties, distinct from their individual components. A striking example is that of DNA-inorganic hybrid micro/nanostructures, fabricated by the rolling circle technique. Here, a novel concept for the encapsulation of bioactive proteins in DNA flowers (DNF) while maintaining the activity of protein payloads is reported. A wide range of proteins, including enzymes, can be simultaneously associated with the growing DNA strands and Mg2 PPi crystals during the rolling circle process, ultimately leading to the direct immobilization of proteins into DNF. The unique porous structure of this construct, along with the abundance of Mg ions and DNA molecules present, provides many interaction sites for proteins, enabling high loading efficiency and enhanced stability. Further, as a proof of concept, it is demonstrated that the DNF can deliver payloads of cytotoxic protein (i.e., RNase A) to the cells without a loss in its biological function and structural integrity, resulting in highly increased cell death compared to the free protein.

JOURNAL ARTICLE

Lin Y, Pashuck ET, Thomas MR, Amdursky N, Wang S-T, Chow LW, Stevens MMet al., 2017, Plasmonic Chirality Imprinting on Nucleobase-Displaying Supramolecular Nanohelices by Metal-Nucleobase Recognition, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 56, Pages: 2361-2365, ISSN: 1433-7851

JOURNAL ARTICLE

Macon ALB, Jacquemin M, Page SJ, Li S, Bertazzo S, Stevens MM, Hanna JV, Jones JRet al., 2017, Lithium-silicate sol-gel bioactive glass and the effect of lithium precursor on structure-property relationships, JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, Vol: 81, Pages: 84-94, ISSN: 0928-0707

JOURNAL ARTICLE

Pacheco-Moreno CM, Schreck M, Scaccabarozzi AD, Bourgun P, Wantz G, Stevens MM, Dautel OJ, Stingelin Net al., 2017, The Importance of Materials Design to Make Ions Flow: Toward Novel Materials Platforms for Bioelectronics Applications, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648

JOURNAL ARTICLE

Parmar PA, St-Pierre J-P, Chow LW, Spicer CD, Stoichevska V, Peng YY, Werkmeister JA, Ramshaw JAM, Stevens MMet al., 2017, Enhanced articular cartilage by human mesenchymal stem cells in enzymatically mediated transiently RGDS-functionalized collagen mimetic hydrogels, ACTA BIOMATERIALIA, Vol: 51, Pages: 75-88, ISSN: 1742-7061

JOURNAL ARTICLE

Pelaz B, Alexiou C, Alvarez -Puebla RA, Alves F, Andrews AM, Ashraf S, Balogh LP, Ballerini L, Bestetti A, Brendel C, Bosi S, Carril M, Chan WCW, Chen C, Chen X, Chen X, Cheng Z, Cui D, Du J, Dullin C, Escudero A, Feliu N, Gao M, George M, Gogotsi Y, Grunweller A, Gu Z, Halas NJ, Hampp N, Hartmann RK, Hersam MC, Hunziker P, Jian J, Jiang X, Jungebluth P, Kadhiresan P, Kataoka K, Khademhosseini A, Kopecek J, Kotov NA, Krug HF, Lee DS, Lehr C-M, Leong KW, Liang X-J, Lim ML, Liz-Marzan LM, Ma X, Macchiarini P, Meng H, Mohwald H, Mulvaney P, Nel AE, Nie S, Nordlander P, Okano T, Oliveira J, Park TH, Penner RM, Prato M, Puntes V, Rotello VM, Samarakoon A, Schaak RE, Shen Y, Sjoqvist S, Skirtach AG, Soliman MG, Stevens MM, Sung H-W, Tang BZ, Tietze R, Udugama BN, VanEpps JS, Weil T, Weiss PS, Willner I, Wu Y, Yang L, Yue Z, Zhang Q, Zhang Q, Zhang X-E, Zhao Y, Zhou X, Parak WJet al., 2017, Diverse Applications of Nanomedicine, ACS NANO, Vol: 11, Pages: 2313-2381, ISSN: 1936-0851

JOURNAL ARTICLE

Speidel AT, Stuckey DJ, Chow LW, Jackson LH, Noseda M, Abreu Paiva M, Schneider MD, Stevens MMet al., 2017, Multimodal Hydrogel-Based Platform To Deliver and Monitor Cardiac Progenitor/Stem Cell Engraftment., ACS Cent Sci, Vol: 3, Pages: 338-348, ISSN: 2374-7943

Retention and survival of transplanted cells are major limitations to the efficacy of regenerative medicine, with short-term paracrine signals being the principal mechanism underlying current cell therapies for heart repair. Consequently, even improvements in short-term durability may have a potential impact on cardiac cell grafting. We have developed a multimodal hydrogel-based platform comprised of a poly(ethylene glycol) network cross-linked with bioactive peptides functionalized with Gd(III) in order to monitor the localization and retention of the hydrogel in vivo by magnetic resonance imaging. In this study, we have tailored the material for cardiac applications through the inclusion of a heparin-binding peptide (HBP) sequence in the cross-linker design and formulated the gel to display mechanical properties resembling those of cardiac tissue. Luciferase-expressing cardiac stem cells (CSC-Luc2) encapsulated within these gels maintained their metabolic activity for up to 14 days in vitro. Encapsulation in the HBP hydrogels improved CSC-Luc2 retention in the mouse myocardium and hind limbs at 3 days by 6.5- and 12- fold, respectively. Thus, this novel heparin-binding based, Gd(III)-tagged hydrogel and CSC-Luc2 platform system demonstrates a tailored, in vivo detectable theranostic cell delivery system that can be implemented to monitor and assess the transplanted material and cell retention.

JOURNAL ARTICLE

Spicer CD, Booth MA, Mawad D, Armgarth A, Nielsen CB, Stevens MMet al., 2017, Synthesis of Hetero-bifunctional, End-Capped Oligo-EDOT Derivatives., Chem, Vol: 2, Pages: 125-138

Conjugated oligomers of 3,4-ethylenedioxythiophene (EDOT) are attractive materials for tissue engineering applications and as model systems for studying the properties of the widely used polymer poly(3,4-ethylenedioxythiophene). We report here the facile synthesis of a series of keto-acid end-capped oligo-EDOT derivatives (n = 2-7) through a combination of a glyoxylation end-capping strategy and iterative direct arylation chain extension. Importantly, these structures not only represent the longest oligo-EDOTs reported but are also bench stable, in contrast to previous reports on such oligomers. The constructs reported here can undergo subsequent derivatization for integration into higher-order architectures, such as those required for tissue engineering applications. The synthesis of hetero-bifunctional constructs, as well as those containing mixed-monomer units, is also reported, allowing further complexity to be installed in a controlled manner. Finally, we describe the optical and electrochemical properties of these oligomers and demonstrate the importance of the keto-acid in determining their characteristics.

JOURNAL ARTICLE

Wang S-T, Lin Y, Todorova N, Xu Y, Mazo M, Rana S, Leonardo V, Amdursky N, Spicer CD, Alexander BD, Edwards AA, Matthews SJ, Yarovsky I, Stevens MMet al., 2017, Facet-Dependent Interactions of Islet Amyloid Polypeptide with Gold Nanoparticles: Implications for Fibril Formation and Peptide-Induced Lipid Membrane Disruption, CHEMISTRY OF MATERIALS, Vol: 29, Pages: 1550-1560, ISSN: 0897-4756

JOURNAL ARTICLE

Amdursky N, Wang X, Meredith P, Bradley DDC, Stevens MMet al., 2016, Long-Range Proton Conduction across Free-Standing Serum Albumin Mats, ADVANCED MATERIALS, Vol: 28, Pages: 2692-2698, ISSN: 0935-9648

JOURNAL ARTICLE

Bell RV, Parkins CC, Young RA, Preuss CM, Stevens MM, Bon SAFet al., 2016, Assembly of emulsion droplets into fibers by microfluidic wet spinning, JOURNAL OF MATERIALS CHEMISTRY A, Vol: 4, Pages: 813-818, ISSN: 2050-7488

JOURNAL ARTICLE

Bergholt MS, St-Pierre J-P, Offeddu GS, Parmar PA, Albro MB, Puetzer JL, Oyen ML, Stevens MMet al., 2016, Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage, ACS CENTRAL SCIENCE, Vol: 2, Pages: 885-895, ISSN: 2374-7943

JOURNAL ARTICLE

Blaeser A, Campos DFD, Puster U, Richtering W, Stevens MM, Fischer Het al., 2016, Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity, ADVANCED HEALTHCARE MATERIALS, Vol: 5, Pages: 326-333, ISSN: 2192-2640

JOURNAL ARTICLE

Campagnolo P, Gormley AJ, Chow LW, Guex AG, Parmar PA, Puetzer JL, Steele JAM, Breant A, Madeddu P, Stevens MMet al., 2016, Pericyte Seeded Dual Peptide Scaffold with Improved Endothelialization for Vascular Graft Tissue Engineering, ADVANCED HEALTHCARE MATERIALS, Vol: 5, Pages: 3046-3055, ISSN: 2192-2640

JOURNAL ARTICLE

Chapman R, Gormley AJ, Stenzel MH, Stevens MMet al., 2016, Combinatorial Low-Volume Synthesis of Well-Defined Polymers by Enzyme Degassing, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 55, Pages: 4500-4503, ISSN: 1433-7851

JOURNAL ARTICLE

Chung JJ, Li S, Stevens MM, Georgiou TK, Jones JRet al., 2016, Tailoring Mechanical Properties of Sol-Gel Hybrids for Bone Regeneration through Polymer Structure, CHEMISTRY OF MATERIALS, Vol: 28, Pages: 6127-6135, ISSN: 0897-4756

JOURNAL ARTICLE

Crowder SW, Leonardo V, Whittaker T, Papathanasiou P, Stevens MMet al., 2016, Material Cues as Potent Regulators of Epigenetics and Stem Cell Function, CELL STEM CELL, Vol: 18, Pages: 39-52, ISSN: 1934-5909

JOURNAL ARTICLE

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