Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

299 results found

Albro M, Bergholt M, St-Pierre JP, Vinals Guitart A, Zlotnick HM, Evita EG, Stevens MMet al., 2018, Raman Spectroscopic Imaging for Quantification of Depth-Dependent and Local Heterogeneities in Native and Engineered Cartilage, npj Regenerative Medicine, Vol: 3, ISSN: 2057-3995

Articular cartilage possesses a remarkable, mechanically-robust extracellular matrix (ECM) that is organized and distributed throughout the tissue to resist physiologic strains and provide low friction during articulation. The ability to characterize the make-up and distribution of the cartilage ECM is critical to both understand the process by which articular cartilage undergoes disease-related degeneration and to develop novel tissue repair strategies to restore tissue functionality. However, the ability to quantitatively measure the spatial distribution of cartilage ECM constituents throughout the tissue has remained a major challenge. In this experimental investigation, we assessed the analytical ability of Raman micro-spectroscopic imaging to semi-quantitatively measure the distribution of the major ECM constituents in cartilage tissues. Raman spectroscopic images were acquired of two distinct cartilage tissue types that possess large spatial ECM gradients throughout their depth: native articular cartilage explants and large engineered cartilage tissue constructs. Spectral acquisitions were processed via multivariate curve resolution to decompose the “fingerprint” range spectra (800–1800 cm−1) to the component spectra of GAG, collagen, and water, giving rise to the depth dependent concentration profile of each constituent throughout the tissues. These Raman spectroscopic acquired-profiles exhibited strong agreement with profiles independently acquired via direct biochemical assaying of spatial tissue sections. Further, we harness this spectroscopic technique to evaluate local heterogeneities through the depth of cartilage. This work represents a powerful analytical validation of the accuracy of Raman spectroscopic imaging measurements of the spatial distribution of biochemical components in a biological tissue and shows that it can be used as a valuable tool for quantitatively measuring the distribution and organization of ECM con

JOURNAL ARTICLE

Bergholt MS, Serio A, McKenzie JS, Boyd A, Soares RF, Tillner J, Chiappini C, Wu V, Dannhorn A, Takats Z, Williams A, Stevens MMet al., 2018, Correlated Heterospectral Lipidomics for Biomolecular Profiling of Remyelination in Multiple Sclerosis, ACS CENTRAL SCIENCE, Vol: 4, Pages: 39-51, ISSN: 2374-7943

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Brangel P, Sobarzo A, Parolo C, Miller BS, Howes PD, Gelkop S, Lutwama JJ, Dye JM, McKendry RA, Lobel L, Stevens MMet al., 2018, A Serological Point-of-Care Test for the Detection of IgG Antibodies against Ebola Virus in Human Survivors., ACS Nano, Vol: 12, Pages: 63-73

Ebola virus disease causes widespread and highly fatal epidemics in human populations. Today, there is still great need for point-of-care tests for diagnosis, patient management and surveillance, both during and post outbreaks. We present a point-of-care test comprising an immunochromatographic strip and a smartphone reader, which detects and semiquantifies Ebola-specific antibodies in human survivors. We developed a Sudan virus glycoprotein monoplex platform and validated it using sera from 90 human survivors and 31 local noninfected controls. The performance of the glycoprotein monoplex was 100% sensitivity and 98% specificity compared to standard whole antigen enzyme-linked immunosorbent assay (ELISA), and it was validated with freshly collected patient samples in Uganda. Moreover, we constructed a multiplex test for simultaneous detection of antibodies against three recombinant Sudan virus proteins. A pilot study comprising 15 survivors and 5 noninfected controls demonstrated sensitivity and specificity of 100% compared to standard ELISA. Finally, we developed a second multiplex subtype assay for the identification of exposure to three related EVD species: Sudan virus, Bundibugyo virus and Ebola virus (formerly Zaire) using recombinant viral glycoprotein. This multiplex test could distinguish between the host's immunity to specific viral species and identify cross-reactive immunity. These developed serological platforms consisted of capture ligands with high specificity and sensitivity, in-house developed strips and a compatible smartphone application. These platforms enabled rapid and portable testing, data storage and sharing as well as geographical tagging of the tested individuals in Uganda. This platform holds great potential as a field tool for diagnosis, vaccine development, and therapeutic evaluation.

JOURNAL ARTICLE

Hsu C-C, Serio A, Amdursky N, Besnard C, Stevens MMet al., 2018, Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications., ACS Appl Mater Interfaces

Neural tissue engineering (TE) represents a promising new avenue of therapy to support nerve recovery and regeneration. To recreate the complex environment in which neurons develop and mature, the ideal biomaterials for neural TE require a number of properties and capabilities including the appropriate biochemical and physical cues to adsorb and release specific growth factors. Here, we present neural TE constructs based on electrospun serum albumin (SA) fibrous scaffolds. We doped our SA scaffolds with an iron-containing porphyrin, hemin, to confer conductivity, and then functionalized them with different recombinant proteins and growth factors to ensure cell attachment and proliferation. We demonstrated the potential for these constructs combining topographical, biochemical, and electrical stimuli by testing them with clinically relevant neural populations derived from human induced pluripotent stem cells (hiPSCs). Our scaffolds could support the attachment, proliferation, and neuronal differentiation of hiPSC-derived neural stem cells (NSCs), and were also able to incorporate active growth factors and release them over time, which modified the behavior of cultured cells and substituted the need for growth factor supplementation by media change. Electrical stimulation on the doped SA scaffold positively influenced the maturation of neuronal populations, with neurons exhibiting more branched neurites compared to controls. Through promotion of cell proliferation, differentiation, and neurite branching of hiPSC-derived NSCs, these conductive SA fibrous scaffolds are of broad application in nerve regeneration strategies.

JOURNAL ARTICLE

Littmann E, Autefage H, Solanki AK, Kallepitis C, Jones JR, Alini M, Peroglio M, Stevens MMet al., 2018, Cobalt-containing bioactive glasses reduce human mesenchymal stem cell chondrogenic differentiation despite HIF-1 alpha stabilisation, JOURNAL OF THE EUROPEAN CERAMIC SOCIETY, Vol: 38, Pages: 877-886, ISSN: 0955-2219

JOURNAL ARTICLE

Loynachan CN, Thomas MR, Gray ER, Richards DA, Kim J, Miller BS, Brookes JC, Agarwal S, Chudasama V, McKendry RA, Stevens MMet al., 2018, Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultrabroad Dynamic Range, ACS NANO, Vol: 12, Pages: 279-288, ISSN: 1936-0851

JOURNAL ARTICLE

Milner PE, Parkes M, Puetzer JL, Chapman R, Stevens MM, Cann P, Jeffers JRTet al., 2018, A low friction, biphasic and boundary lubricating hydrogel for cartilage replacement, ACTA BIOMATERIALIA, Vol: 65, Pages: 102-111, ISSN: 1742-7061

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Rizzo R, Alvaro M, Danz N, Napione L, Descrovi E, Schmieder S, Sinibaldi A, Chandrawati R, Rana S, Munzert P, Schubert T, Maillart E, Anopchenko A, Rivolo P, Mascioletti A, Sonntag F, Stevens MM, Bussolino F, Michelotti Fet al., 2018, Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices, SENSORS AND ACTUATORS B-CHEMICAL, Vol: 255, Pages: 2143-2150, ISSN: 0925-4005

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Amdursky N, Wang X, Meredith P, Riley DJ, Payne DJ, Bradley DDC, Stevens MMet al., 2017, Electron Hopping Across Hemin-Doped Serum Albumin Mats on Centimeter-Length Scales, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648

JOURNAL ARTICLE

Armstrong JPK, Holme MN, Stevens MM, 2017, Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics, ACS NANO, Vol: 11, Pages: 69-83, ISSN: 1936-0851

JOURNAL ARTICLE

Bergholt MS, Albro MB, Stevens MM, 2017, Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy, BIOMATERIALS, Vol: 140, Pages: 128-137, ISSN: 0142-9612

JOURNAL ARTICLE

Chan WCW, Chhowalla M, Glotzer S, Gogotsi Y, Hafner JH, Hammond PT, Hersam MC, Javey A, Kagan CR, Kataoka K, Khademhosseini A, Kotov NA, Lee S-T, Li Y, Moehwald H, Mulvaney P, Nel AE, Nordlander PJ, Parak WJ, Penner RM, Rogach AL, Schaak RE, Stevens MM, Wee ATS, Willson CG, Fernandez LEE, Weiss PSet al., 2017, Our First and Next Decades at ACS Nano, Publisher: AMER CHEMICAL SOC

OTHER

Chandrawati R, Chang JYH, Reina-Torres E, Jumeaux C, Sherwood JM, Stamer WD, Zelikin AN, Overby DR, Stevens MMet al., 2017, Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma, ADVANCED MATERIALS, Vol: 29, ISSN: 0935-9648

JOURNAL ARTICLE

Chandrawati R, Olesen MTJ, Marini TCC, Bisra G, Guex AG, de Oliveira MG, Zelikin AN, Stevens MMet al., 2017, Enzyme Prodrug Therapy Engineered into Electrospun Fibers with Embedded Liposomes for Controlled, Localized Synthesis of Therapeutics, ADVANCED HEALTHCARE MATERIALS, Vol: 6, ISSN: 2192-2640

JOURNAL ARTICLE

Chang JYH, Chow LW, Dismuke WM, Ethier CR, Stevens MM, Stamer WD, Overby DRet al., 2017, Peptide-Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite-Mediated Nitration, ADVANCED HEALTHCARE MATERIALS, Vol: 6, ISSN: 2192-2640

JOURNAL ARTICLE

Chow A, Stuckey DJ, Kidher E, Rocco M, Jabbour RJ, Mansfield CA, Darzi A, Harding SE, Stevens MM, Athanasiou Tet al., 2017, Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Encapsulating Bioactive Hydrogels Improve Rat Heart Function Post Myocardial Infarction, STEM CELL REPORTS, Vol: 9, Pages: 1415-1422, ISSN: 2213-6711

JOURNAL ARTICLE

Chung JJ, Fujita Y, Li S, Stevens MM, Kasuga T, Georgiou TK, Jones JRet al., 2017, Biodegradable inorganic-organic hybrids of methacrylate star polymers for bone regeneration, ACTA BIOMATERIALIA, Vol: 54, Pages: 411-418, ISSN: 1742-7061

JOURNAL ARTICLE

Chung JJ, Sum BST, Li S, Stevens MM, Georgiou TK, Jones JRet al., 2017, Effect of Comonomers on Physical Properties and Cell Attachment to Silica-Methacrylate/Acrylate Hybrids for Bone Substitution, MACROMOLECULAR RAPID COMMUNICATIONS, Vol: 38, ISSN: 1022-1336

JOURNAL ARTICLE

Clarke DE, Pashuck ET, Bertazzo S, Weaver JVM, Stevens MMet al., 2017, Self-Healing, Self-Assembled beta-Sheet Peptide Poly(gamma-glutamic acid) Hybrid Hydrogels, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 139, Pages: 7250-7255, ISSN: 0002-7863

JOURNAL ARTICLE

Ehademhosseini A, Chan WWC, Chhowalla M, Glotzer SC, Gogotsi Y, Hafner JH, Hammond PT, Hersam MC, Javey A, Kagan CR, Kotov NA, Lee S-T, Li Y, Mohwald H, Mulvaney PA, Nel AE, Parak WJ, Penner RM, Rogach AL, Schaak RE, Stevens MM, Wee ATS, Brinker J, Chen X, Chi L, Crommie M, Dekker C, Farokhzad O, Gerber C, Ginger DS, Irvine DJ, Kiessling LL, Kostarelos K, Landes C, Lee T, Leggett GJ, Liang X-J, Liz-Marzan L, Millstone J, Odom TW, Ozcan A, Prato M, Rao CNR, Sailor MJ, Weiss E, Weiss PSet al., 2017, Nanoscience and Nanotechnology Cross Borders, Publisher: AMER CHEMICAL SOC

OTHER

Ember KJI, Hoeve MA, McAughtrie SL, Bergholt MS, Dwyer BJ, Stevens MM, Faulds K, Forbes SJ, Campbell CJet al., 2017, Raman spectroscopy and regenerative medicine: a review, NPJ REGENERATIVE MEDICINE, Vol: 2, ISSN: 2057-3995

JOURNAL ARTICLE

Fiocco L, Li S, Stevens MM, Bernardo E, Jones JRet al., 2017, Biocompatibility and bioactivity of porous polymer-derived Ca-Mg silicate ceramics, ACTA BIOMATERIALIA, Vol: 50, Pages: 56-67, ISSN: 1742-7061

JOURNAL ARTICLE

Guex AG, Puetzer JL, Armgarth A, Littmann E, Stavrinidou E, Giannelis EP, Malliaras GG, Stevens MMet al., 2017, Highly porous scaffolds of PEDOT:PSS for bone tissue engineering, ACTA BIOMATERIALIA, Vol: 62, Pages: 91-101, ISSN: 1742-7061

JOURNAL ARTICLE

Guex AG, Spicer CD, Armgarth A, Gelmi A, Humphrey EJ, Terracciano CM, Harding SE, Stevens MMet al., 2017, Electrospun aniline-tetramer-co-polycaprolactone fibers for conductive, biodegradable scaffolds, MRS COMMUNICATIONS, Vol: 7, Pages: 375-382, ISSN: 2159-6859

JOURNAL ARTICLE

Hall CE, Yao Z, Choi M, Tyzack GE, Serio A, Luisier R, Harley J, Preza E, Arber C, Crisp SJ, Watson PMD, Kullmann DM, Abramov AY, Wray S, Burley R, Loh SHY, Martins LM, Stevens MM, Luscombe NM, Sibley CR, Lakatos A, Ule J, Gandhi S, Patani Ret al., 2017, Progressive Motor Neuron Pathology and the Role of Astrocytes in a Human Stem Cell Model of VCP-Related ALS, CELL REPORTS, Vol: 19, Pages: 1739-1749, ISSN: 2211-1247

JOURNAL ARTICLE

He M, Callanan A, Lagaras K, Steele JAM, Stevens MMet al., 2017, Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, Vol: 105, Pages: 1352-1360, ISSN: 1552-4973

JOURNAL ARTICLE

Higgins SG, Stevens MM, 2017, Extracting the contents of living cells, SCIENCE, Vol: 356, Pages: 379-380, ISSN: 0036-8075

JOURNAL ARTICLE

Horejs C-M, St-Pierre J-P, Ojala JRM, Steele JAM, da Silva PB, Rynne-Vidal A, Maynard SA, Hansel CS, Rodriguez-Fernandez C, Mazo MM, You AYF, Wang AJ, von Erlach T, Tryggvason K, Lopez-Cabrera M, Stevens MMet al., 2017, Preventing tissue fibrosis by local biomaterials interfacing of specific cryptic extracellular matrix information, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723

JOURNAL ARTICLE

Jumeaux C, Wahlsten O, Block S, Kim E, Chandrawati R, Howes PD, Höök F, Stevens MMet al., 2017, MicroRNA Detection by DNA-Mediated Liposome Fusion., Chembiochem

Membrane fusion is a process of fundamental importance in biological systems that involves highly selective recognition mechanisms for the trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection because relatively few highly discriminating targets lead to fusion and an accompanied engagement of a large payload of signal-generating molecules. In this work, sequence-specific DNA-mediated liposome fusion is used for the highly selective detection of microRNA. The detection of miR-29a, a known flu biomarker, is demonstrated down to 18 nm within 30 min with high specificity by using a standard laboratory microplate reader. Furthermore, one order of magnitude improvement in the limit of detection is demonstrated by using a novel imaging technique combined with an intensity fluctuation analysis, which is coined two-color fluorescence correlation microscopy.

JOURNAL ARTICLE

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