Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Amdursky:2018:10.1039/C8TB01014E,
author = {Amdursky, N and Mazo, M and Thomas, MR and Humphrey, E and Puetzer, J and St-Pierre, J and Skaalure, S and Richardson, RM and Terracciano, C and Stevens, MM},
doi = {10.1039/C8TB01014E},
journal = {Journal of Materials Chemistry B},
pages = {5604--5612},
title = {Elastic serum-albumin based hydrogels: mechanism of formation and application in cardiac tissue engineering},
url = {http://dx.doi.org/10.1039/C8TB01014E},
volume = {6},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Hydrogels are promising materials for mimicking the extra-cellular environment. Here, we  present a  simple  methodology  for the formation  of a  free-standing viscoelastic hydrogel  from the  abundant  and  low  cost  protein serum  albumin. We show  that  the mechanical properties of the hydrogel exhibit a complicated behaviour as a function of the weight fraction of the protein component. We further use X-ray scattering to shed light on the mechanism  of  gelation from the formation of a fibrillary  network at low weight  fractions  to  interconnected  aggregates  at  higher  fractions.  Given  the  match between our hydrogel  elasticity and that  of  the  myocardium,  we  investigated its potential for supporting cardiac cells in vitro. Interestingly, the sehydrogels support the formation of several layers of myocytes and significantly promote the maintenance of a native-likegene  expression  profile compared  to  those  cultured  on glass. When confronted with a multicellular ventricular cell preparation, the hydrogels can support macroscopically  contracting cardiac-like tissues with a distinct cell arrangement, and form mm-long vascular-like structures. We envisage that our simple approach for the formation  of an  elastic substrate from  an  abundant  protein  makes the hydrogel a compelling biomedical material candidate for a wide range of cell types.
AU  - Amdursky,N
AU  - Mazo,M
AU  - Thomas,MR
AU  - Humphrey,E
AU  - Puetzer,J
AU  - St-Pierre,J
AU  - Skaalure,S
AU  - Richardson,RM
AU  - Terracciano,C
AU  - Stevens,MM
DO  - 10.1039/C8TB01014E
EP  - 5612
PY  - 2018///
SN  - 2050-750X
SP  - 5604
TI  - Elastic serum-albumin based hydrogels: mechanism of formation and application in cardiac tissue engineering
T2  - Journal of Materials Chemistry B
UR  - http://dx.doi.org/10.1039/C8TB01014E
UR  - http://hdl.handle.net/10044/1/62006
VL  - 6
ER  -
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