Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2018:10.1021/acsami.8b07250,
author = {Wang, Y and Howes, P and Kim, E and Spicer, C and Thomas, M and Lin, Y and Crowder, S and Pence, I and Stevens, MM},
doi = {10.1021/acsami.8b07250},
journal = {ACS Applied Materials and Interfaces},
pages = {28290--28300},
title = {Duplex specific nuclease-amplified detection of microRNA using compact quantum dot-DNA conjugates},
url = {http://dx.doi.org/10.1021/acsami.8b07250},
volume = {10},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Advances in nanotechnology have provided new opportunities for the design of next-generation nucleic acid biosensors and diagnostics. Indeed, combining advances in functional nanoparticles, DNA nanotechnology, and nuclease-enzyme-based amplification can give rise to new assays with advantageous properties. In this work, we developed a microRNA (miRNA) assay using bright fluorescent quantum dots (QDs), simple DNA probes, and the enzyme duplex-specific nuclease. We employed an isothermal target-recycling mechanism, where a single miRNA target triggers the cleavage of many DNA signal probes. The incorporation of DNA-functionalized QDs enabled a quantitative fluorescent readout, mediated by Förster resonance energy transfer (FRET)-based interaction with the DNA signal probes. Our approach splits the reaction in two, performing the enzyme-mediated amplification and QD-based detection steps separately such that each reaction could be optimized for performance of the active components. Target recycling gave ca. 3 orders of magnitude amplification, yielding highly sensitive detection with a limit of 42 fM (or 1.2 amol) of miR-148, with excellent selectivity versus mismatched sequences and other miRNAs. Furthermore, we used an alternative target (miR-21) and FRET pair for direct and absolute quantification of miR-21 in RNA extracts from human cancer and normal cell lines.
AU  - Wang,Y
AU  - Howes,P
AU  - Kim,E
AU  - Spicer,C
AU  - Thomas,M
AU  - Lin,Y
AU  - Crowder,S
AU  - Pence,I
AU  - Stevens,MM
DO  - 10.1021/acsami.8b07250
EP  - 28300
PY  - 2018///
SN  - 1944-8244
SP  - 28290
TI  - Duplex specific nuclease-amplified detection of microRNA using compact quantum dot-DNA conjugates
T2  - ACS Applied Materials and Interfaces
UR  - http://dx.doi.org/10.1021/acsami.8b07250
UR  - http://hdl.handle.net/10044/1/62689
VL  - 10
ER  -
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