Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nele:2020:10.1002/adma.201905914,
author = {Nele, V and Schutt, CE and Wojciechowski, J and Kit-Anan, W and Doutch, JJ and Armstrong, J and Stevens, M},
doi = {10.1002/adma.201905914},
journal = {Advanced Materials},
pages = {1--8},
title = {Ultrasound-triggered enzymatic gelation},
url = {http://dx.doi.org/10.1002/adma.201905914},
volume = {32},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Hydrogels are formed using various triggers, including light irradiation, pH adjustment, heating,cooling or chemical addition. In this report, a new method for forming hydrogels is introduced:ultrasound-triggered enzymatic gelation. Specifically, ultrasound is used as a stimulus to liberateliposomal calcium ions, which then trigger the enzymatic activity of transglutaminase. Theactivated enzyme catalyzes the formation of fibrinogen hydrogels through covalent intermolecularcrosslinking. The catalysis and gelation processes are monitored in real time and both the enzymekinetics and final hydrogel properties are controlled by varying the initial ultrasound exposure time.This technology is extended to microbubble-liposome conjugates, which exhibit a stronger responseto the applied acoustic field and are also used for ultrasound-triggered enzymatic hydrogelation. Tothe best of our knowledge, these results are the first instance in which ultrasound has been used as atrigger for either enzyme catalysis or enzymatic hydrogelation. This approach is highly versatile and Peer reviewed version of the manuscript published in final form at Advanced Materials (2020)2could be readily applied to different ion-dependent enzymes or gelation systems. Moreover, thiswork paves the way for the use of ultrasound as a remote trigger for in vivo hydrogelation.
AU  - Nele,V
AU  - Schutt,CE
AU  - Wojciechowski,J
AU  - Kit-Anan,W
AU  - Doutch,JJ
AU  - Armstrong,J
AU  - Stevens,M
DO  - 10.1002/adma.201905914
EP  - 8
PY  - 2020///
SN  - 0935-9648
SP  - 1
TI  - Ultrasound-triggered enzymatic gelation
T2  - Advanced Materials
UR  - http://dx.doi.org/10.1002/adma.201905914
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201905914
UR  - http://hdl.handle.net/10044/1/75484
VL  - 32
ER  -
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