Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Seong:2020:10.1021/acsnano.9b08689,
author = {Seong, H and Higgins, SG and Penders, J and Armstrong, JPK and Crowder, SW and Moore, AC and Sero, JE and Becce, M and Stevens, MM},
doi = {10.1021/acsnano.9b08689},
journal = {ACS Nano},
pages = {5371--5381},
title = {Size-tunable nanoneedle arrays for influencing stem cell morphology, gene expression and nuclear membrane curvature},
url = {http://dx.doi.org/10.1021/acsnano.9b08689},
volume = {14},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - High-aspect-ratio nanostructures have emerged as versatile platforms for intracellular sensing and biomolecule delivery. Here, we present a microfabrication approach in which a combination of reactive ion etching protocols was used to produce high-aspect-ratio, nondegradable silicon nanoneedle arrays with tip diameters that can be finely tuned between 20 and 700 nm. We used these arrays to guide the long-term culture of human mesenchymal stem cells (hMSCs). Notably, we used the nanoneedle tip diameter to control the morphology, nuclear size and F-actin alignment of interfaced hMSCs, and to regulate the expression of nuclear lamina genes, Yes-associated protein (YAP) target genes and focal adhesion genes. These topography-driven changes were attributed to signaling by Rho-family GTPase pathways, differences in the effective stiffness of the nanoneedle arrays and the degree of nuclear membrane impingement, with the latter clearly visualized using focused-ion beam scanning electron microscopy (FIB-SEM). Our approach to design high-aspect-ratio nanostructures will be broadly applicable to design biomaterials and biomedical devices used for long-term cell stimulation and monitoring.
AU  - Seong,H
AU  - Higgins,SG
AU  - Penders,J
AU  - Armstrong,JPK
AU  - Crowder,SW
AU  - Moore,AC
AU  - Sero,JE
AU  - Becce,M
AU  - Stevens,MM
DO  - 10.1021/acsnano.9b08689
EP  - 5381
PY  - 2020///
SN  - 1936-0851
SP  - 5371
TI  - Size-tunable nanoneedle arrays for influencing stem cell morphology, gene expression and nuclear membrane curvature
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.9b08689
UR  - https://pubs.acs.org/doi/10.1021/acsnano.9b08689
UR  - http://hdl.handle.net/10044/1/78532
VL  - 14
ER  -
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