Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Maynard:2020:10.1021/acsnano.0c07428,
author = {Maynard, S and Gelmi, A and Skaalure, S and Pence, I and Lee-Reeves, C and Sero, J and Whittaker, T and Stevens, M},
doi = {10.1021/acsnano.0c07428},
journal = {ACS Nano},
pages = {17321--17332},
title = {Nanoscale molecular quantification of stem cell-hydrogel interactions},
url = {http://dx.doi.org/10.1021/acsnano.0c07428},
volume = {14},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A common approach to tailoring synthetic hydrogels for regenerative medicine applications involves incorporating RGD cell adhesion peptides, yet assessing the cellular response to engineered microenvironments at the nanoscale remains challenging. To date, no study has demonstrated how RGD concentration in hydrogels affects the presentation of individual cell surface receptors. Here we studied the interaction between human mesenchymal stem cells (hMSCs) and RGD-functionalized poly(ethylene glycol) hydrogels, by correlating macro- and nanoscale single-cell interfacial quantification techniques. We quantified RGD unbinding forces on a synthetic hydrogel using single cell atomic force spectroscopy, revealing that short-term binding of hMSCs was sensitive to RGD concentration. We also performed direct stochastic optical reconstruction microscopy (dSTORM) to quantify the molecular interactions between integrin α5β1 and a biomaterial, unexpectedly revealing that increased integrin clustering at the hydrogel-cell interface correlated with fewer available RGD binding sites. Our complementary, quantitative approach uncovered mechanistic insights into specific stem cell-hydrogel interactions, where dSTORM provides nanoscale sensitivity to RGD-dependent differences in cell surface localization of integrin α5β1. Our findings reveal that it is possible to precisely determine how peptide-functionalized hydrogels interact with cells at the molecular scale, thus providing a basis to fine-tune the spatial presentation of bioactive ligands.
AU  - Maynard,S
AU  - Gelmi,A
AU  - Skaalure,S
AU  - Pence,I
AU  - Lee-Reeves,C
AU  - Sero,J
AU  - Whittaker,T
AU  - Stevens,M
DO  - 10.1021/acsnano.0c07428
EP  - 17332
PY  - 2020///
SN  - 1936-0851
SP  - 17321
TI  - Nanoscale molecular quantification of stem cell-hydrogel interactions
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.0c07428
UR  - http://hdl.handle.net/10044/1/85779
VL  - 14
ER  -
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