Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Stevens:2016:10.1039/C6NR00484A,
author = {Stevens, MM and Gliddon, H and Howes, P and Kaforou, M and Levin, M},
doi = {10.1039/C6NR00484A},
journal = {Nanoscale},
pages = {10087--10095},
title = {A nucleic acid strand displacement system for the multiplexed detection of tuberculosis-specific mRNA using quantum dots},
url = {http://dx.doi.org/10.1039/C6NR00484A},
volume = {8},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The development of rapid, robust and high performance point-of-care diagnostics relies on the advancement and combination of various areas of research. We have developed an assay for the detection of multiple mRNA molecules that combines DNA nanotechnology with fluorescent nanomaterials. The core switching mechanism is toehold-mediated strand displacement. We have used fluorescent quantum dots (QDs) as signal transducers in this assay, as they bring many benefits including bright fluorescence and multiplexing abilities. The resulting assay is capable of multiplexed detection of long RNA targets against a high concentration background of non-target RNAs, with high sensitivity and specificity and limits of detection in the nanomolar range using only a standard laboratory plate reader. We demonstrate the utility of our QD-based system for the detection of two genes selected from a microarray-derived tuberculosis-specific gene expression signature. Levels of up- and downregulated gene transcripts comprising this signature can be combined to give a disease risk score, making the signature more amenable for use as a diagnostic marker. Our QD-based approach to detect these transcripts could pave the way for novel diagnostic assays for tuberculosis.
AU  - Stevens,MM
AU  - Gliddon,H
AU  - Howes,P
AU  - Kaforou,M
AU  - Levin,M
DO  - 10.1039/C6NR00484A
EP  - 10095
PY  - 2016///
SN  - 2040-3372
SP  - 10087
TI  - A nucleic acid strand displacement system for the multiplexed detection of tuberculosis-specific mRNA using quantum dots
T2  - Nanoscale
UR  - http://dx.doi.org/10.1039/C6NR00484A
UR  - http://hdl.handle.net/10044/1/29833
VL  - 8
ER  -
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