Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Stevens:2016:10.1002/adhm.201600699,
author = {Stevens, MM and Campagnolo, P and Gormley, AJ and Chow, L and Guex, A and Parmar, P and Puetzer, JL and Steele, JAM and Breant, A and Madeddu, P},
doi = {10.1002/adhm.201600699},
journal = {Advanced Healthcare Materials},
pages = {3046--3055},
title = {Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering},
url = {http://dx.doi.org/10.1002/adhm.201600699},
volume = {5},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which exposes to the risk of thrombosis, and lack of native cellular constituents, which favours pathological remodelling. Here, an bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin-derived peptide (Adh) on the ‘luminal’ side and a heparin-binding peptide (Hep) on the ‘abluminal’ side. Additionally, the ‘abluminal’ side of the scaffold is seeded with saphenous vein-derived pericytes (SVPs) as a source of pro-angiogenic growth factors. The Adh peptide significantly increase endothelial cell adhesion, while the Hep peptide promote accumulation of vascular endothelial growth factor (VEGF) secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the ‘abluminal’/Hep side of the scaffold further increase endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Lastly, SVP-seeded scaffolds are preserved by freezing in a xeno-free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient-derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.
AU  - Stevens,MM
AU  - Campagnolo,P
AU  - Gormley,AJ
AU  - Chow,L
AU  - Guex,A
AU  - Parmar,P
AU  - Puetzer,JL
AU  - Steele,JAM
AU  - Breant,A
AU  - Madeddu,P
DO  - 10.1002/adhm.201600699
EP  - 3055
PY  - 2016///
SN  - 2192-2640
SP  - 3046
TI  - Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering
T2  - Advanced Healthcare Materials
UR  - http://dx.doi.org/10.1002/adhm.201600699
UR  - http://hdl.handle.net/10044/1/41268
VL  - 5
ER  -
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