Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bergholt:2017:10.1016/j.biomaterials.2017.06.015,
author = {Bergholt, M and Albro, M and Stevens, MM},
doi = {10.1016/j.biomaterials.2017.06.015},
journal = {Biomaterials},
pages = {128--137},
title = {Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy},
url = {http://dx.doi.org/10.1016/j.biomaterials.2017.06.015},
volume = {140},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tissue engineering (TE) has the potential to improve the outcome for patients with osteoarthritis (OA). The successful clinical translation of this technique as part of a therapy requires the ability to measure extracellular matrix (ECM) production of engineered tissues in vitro, in order to ensure quality control and improve the likelihood of tissue survival upon implantation. Conventional techniques for assessing the ECM content of engineered cartilage, such as biochemical assays and histological staining are inherently destructive. Raman spectroscopy, on the other hand, represents a non-invasive technique for in situ biochemical characterization. Here, we outline current roadblocks in translational Raman spectroscopy in TE and introduce a comprehensive workflow designed to non-destructively monitor and quantify ECM biomolecules in large (>3 mm), live cell TE constructs online. Diffuse near-infrared fiber-optic Raman spectra were measured from live cell cartilaginous TE constructs over a 56-day culturing period. We developed a multivariate curve resolution model that enabled quantitative biochemical analysis of the TE constructs. Raman spectroscopy was able to non-invasively quantify the ECM components and showed an excellent correlation with biochemical assays for measurement of collagen (R2 = 0.84) and glycosaminoglycans (GAGs) (R2 = 0.86). We further demonstrated the robustness of this technique for online prospective analysis of live cell TE constructs. The fiber-optic Raman spectroscopy strategy developed in this work offers the ability to non-destructively monitor construct growth online and can be adapted to a broad range of TE applications in regenerative medicine toward controlled clinical translation.
AU  - Bergholt,M
AU  - Albro,M
AU  - Stevens,MM
DO  - 10.1016/j.biomaterials.2017.06.015
EP  - 137
PY  - 2017///
SN  - 1878-5905
SP  - 128
TI  - Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy
T2  - Biomaterials
UR  - http://dx.doi.org/10.1016/j.biomaterials.2017.06.015
UR  - http://hdl.handle.net/10044/1/49140
VL  - 140
ER  -
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