Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Loynachan:2017:10.1021/acsnano.7b06229,
author = {Loynachan, C and Thomas, MR and Gray, ER and Richards, DA and Kim, J and MIller, BS and Brookes, JC and Chudasama, V and McKendry, RA and Stevens, MM},
doi = {10.1021/acsnano.7b06229},
journal = {ACS Nano},
pages = {279--288},
title = {Platinum nanocatalyst amplification: redefining the gold standard for lateral flow immunoassays with ultra-broad dynamic range},
url = {http://dx.doi.org/10.1021/acsnano.7b06229},
volume = {12},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Paper-based lateral flow immunoassays (LFIAs) are one of the most widely used point-of-care (PoC) devices; however, their application in early disease diagnostics is often limited due to insufficient sensitivity for the requisite sample sizes and the short time frames of PoC testing. To address this, we developed a serum-stable, nanoparticle catalyst-labeled LFIA with a sensitivity surpassing that of both current commercial and published sensitivities for paper-based detection of p24, one of the earliest and most conserved biomarkers of HIV. We report the synthesis and characterization of porous platinum core–shell nanocatalysts (PtNCs), which show high catalytic activity when exposed to complex human blood serum samples. We explored the application of antibody-functionalized PtNCs with strategically and orthogonally modified nanobodies with high affinity and specificity toward p24 and established the key larger nanoparticle size regimes needed for efficient amplification and performance in LFIA. Harnessing the catalytic amplification of PtNCs enabled naked-eye detection of p24 spiked into sera in the low femtomolar range (ca. 0.8 pg·mL–1) and the detection of acute-phase HIV in clinical human plasma samples in under 20 min. This provides a versatile absorbance-based and rapid LFIA with sensitivity capable of significantly reducing the HIV acute phase detection window. This diagnostic may be readily adapted for detection of other biomolecules as an ultrasensitive screening tool for infectious and noncommunicable diseases and can be capitalized upon in PoC settings for early disease detection.
AU  - Loynachan,C
AU  - Thomas,MR
AU  - Gray,ER
AU  - Richards,DA
AU  - Kim,J
AU  - MIller,BS
AU  - Brookes,JC
AU  - Chudasama,V
AU  - McKendry,RA
AU  - Stevens,MM
DO  - 10.1021/acsnano.7b06229
EP  - 288
PY  - 2017///
SN  - 1936-0851
SP  - 279
TI  - Platinum nanocatalyst amplification: redefining the gold standard for lateral flow immunoassays with ultra-broad dynamic range
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.7b06229
UR  - https://pubs.acs.org/doi/10.1021/acsnano.7b06229
UR  - http://hdl.handle.net/10044/1/54786
VL  - 12
ER  -
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