Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rizzo:2018:10.1364/BOE.9.000529,
author = {Rizzo, R and Alvaro, M and Danz, N and Napione, L and Descrovi, E and Schmieder, S and Sinibaldi, A and Rana, S and Chandrawati, R and Munzert, P and Schubert, T and Maillart, E and Anopchenko, A and Rivolo, P and Mascioletti, A and Förster, E and Sonntag, F and Stevens, MM and Bussolino, F and Michelotti, F},
doi = {10.1364/BOE.9.000529},
journal = {Biomedical Optics Express},
pages = {529--542},
title = {Bloch surface wave enhanced biosensor for the direct detection of angiopoietin-2 tumor biomarker in human plasma},
url = {http://dx.doi.org/10.1364/BOE.9.000529},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Quantitative detection of angiogenic biomarkers provides a powerful tool to diagnose cancers in early stages and to follow its progression during therapy. Conventional tests require trained personnel, dedicated laboratory equipment and are generally time-consuming. Herein, we propose our developed biosensing platform as a useful tool for a rapid determination of Angiopoietin-2 biomarker directly from patient plasma within 30 minutes, without any sample preparation or dilution. Bloch surface waves supported by one dimensional photonic crystal are exploited to enhance and redirect the fluorescence arising from a sandwich immunoassay that involves Angiopoietin-2. The sensing units consist of disposable and low-cost plastic biochips coated with the photonic crystal. The biosensing platform is demonstrated to detect Angiopoietin-2 in plasma samples at the clinically relevant concentration of 6 ng/mL, with an estimated limit of detection of approximately 1 ng/mL. This is the first Bloch surface wave based assay capable of detecting relevant concentrations of an angiogenic factor in plasma samples. The results obtained by the developed biosensing platform are in close agreement with enzyme-linked immunosorbent assays, demonstrating a good accuracy, and their repeatability showed acceptable relative variations.
AU  - Rizzo,R
AU  - Alvaro,M
AU  - Danz,N
AU  - Napione,L
AU  - Descrovi,E
AU  - Schmieder,S
AU  - Sinibaldi,A
AU  - Rana,S
AU  - Chandrawati,R
AU  - Munzert,P
AU  - Schubert,T
AU  - Maillart,E
AU  - Anopchenko,A
AU  - Rivolo,P
AU  - Mascioletti,A
AU  - Förster,E
AU  - Sonntag,F
AU  - Stevens,MM
AU  - Bussolino,F
AU  - Michelotti,F
DO  - 10.1364/BOE.9.000529
EP  - 542
PY  - 2018///
SN  - 2156-7085
SP  - 529
TI  - Bloch surface wave enhanced biosensor for the direct detection of angiopoietin-2 tumor biomarker in human plasma
T2  - Biomedical Optics Express
UR  - http://dx.doi.org/10.1364/BOE.9.000529
UR  - http://hdl.handle.net/10044/1/57253
VL  - 9
ER  -
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