Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sigmundsson:2018:10.1016/j.matbio.2018.03.018,
author = {Sigmundsson, K and Ojala, JRM and Öhman, MK and Österholm, A-M and Moreno-Moral, A and Domogatskaya, A and Chong, LY and Sun, Y and Chai, X and Steele, JAM and George, B and Patarroyo, M and Nilsson, A-S and Rodin, S and Ghosh, S and Stevens, MM and Petretto, E and Tryggvason, K},
doi = {10.1016/j.matbio.2018.03.018},
journal = {Matrix Biology},
pages = {5--19},
title = {Culturing functional pancreatic islets on α5-laminins and curative transplantation to diabetic mice},
url = {http://dx.doi.org/10.1016/j.matbio.2018.03.018},
volume = {70},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The efficacy of islet transplantation for diabetes treatment suffers from lack of cadaver-derived islets, islet necrosis and long transfer times prior to transplantation. Here, we developed a method for culturing mouse and human islets in vitro on α5-laminins, which are natural components of islet basement membranes. Adhering islets spread to form layers of 1-3 cells in thickness and remained normoxic and functional for at least 7days in culture. In contrast, spherical islets kept in suspension developed hypoxia and central necrosis within 16h. Transplantation of 110-150 mouse islets cultured on α5-laminin-coated polydimethylsiloxane membranes for 3-7days normalized blood glucose already within 3days in mice with streptozotocin-induced diabetes. RNA-sequencing of isolated and cultured mouse islets provided further evidence for the adhesion and spreading achieved with α5-laminin. Our results suggest that use of such in vitro expanded islets may significantly enhance the efficacy of islet transplantation treatment for diabetes.
AU  - Sigmundsson,K
AU  - Ojala,JRM
AU  - Öhman,MK
AU  - Österholm,A-M
AU  - Moreno-Moral,A
AU  - Domogatskaya,A
AU  - Chong,LY
AU  - Sun,Y
AU  - Chai,X
AU  - Steele,JAM
AU  - George,B
AU  - Patarroyo,M
AU  - Nilsson,A-S
AU  - Rodin,S
AU  - Ghosh,S
AU  - Stevens,MM
AU  - Petretto,E
AU  - Tryggvason,K
DO  - 10.1016/j.matbio.2018.03.018
EP  - 19
PY  - 2018///
SN  - 0945-053X
SP  - 5
TI  - Culturing functional pancreatic islets on α5-laminins and curative transplantation to diabetic mice
T2  - Matrix Biology
UR  - http://dx.doi.org/10.1016/j.matbio.2018.03.018
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29601863
UR  - http://hdl.handle.net/10044/1/59225
VL  - 70
ER  -
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