Imperial College London
Alternate

DrMichaelThemis

Faculty of Natural Sciences, Department of Life Sciences (Silwood Park)

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)1895 267 252m.themis

 
 
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Location

 

Workspace 15Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kennea:2009,
author = {Kennea, NL and Waddington, SN and Chan, J and ODonoghue, K and Yeung, D and Taylor, DL and Al-Alaf, FA and Pirianov, G and Themis, M and Edwards, AD and Fisk, NM and Mehmet, H},
journal = {Cell Cycle},
pages = {1069--1079},
title = {Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19270512},
volume = {7},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The potential of mesenchymal stem cells (MSC) to differentiate into neural lineages has raised the possibility of autologous cell transplantation as a therapy for neurodegenerative diseases. We have identified a population of circulating human fetal mesenchymal stem cells (hfMSC) that are highly proliferative and can readily differentiate into mesodermal lineages such as bone, cartilage, fat and muscle. Here, we demonstrate for the first time that primary hfMSC can differentiate into cells with an oligodendrocyte phenotype both in vitro and in vivo. By exposing hfMSC to neuronal conditioned medium or by introducing the pro-oligodendrocyte gene, Olig-2, hfMSC adopted an oligodendrocyte-like morphology, expressed oligodendrocyte markers and appeared to mature appropriately in culture. Importantly we also demonstrate the differentiation of a clonal population of hfMSC into both mesodermal (bone) and ectodermal (oligodendrocyte) lineages. In the developing murine brain transplanted hfMSC integrated into the parenchyma but oligodendrocyte differentiation of these naïve hfMSC was very low. However, the proportion of cells expressing oligodendrocyte markers increased significantly (from 0.2% to 4%) by preexposing the cells to differentiation medium in vitro prior to transplantation. Importantly, the process of in vivo differentiation occurred without cell fusion. These findings suggest that hfMSC may provide a potential source of oligodendrocytes for study and potential therapy.
AU  - Kennea,NL
AU  - Waddington,SN
AU  - Chan,J
AU  - ODonoghue,K
AU  - Yeung,D
AU  - Taylor,DL
AU  - Al-Alaf,FA
AU  - Pirianov,G
AU  - Themis,M
AU  - Edwards,AD
AU  - Fisk,NM
AU  - Mehmet,H
EP  - 1079
PY  - 2009///
SP  - 1069
TI  - Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype.
T2  - Cell Cycle
UR  - http://www.ncbi.nlm.nih.gov/pubmed/19270512
VL  - 7
ER  -
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