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@article{Mellor:2004:10.1111/j.1478-3231.2004.0929.x,
author = {Mellor, N and Themis, M and Selden, C and Jones, M and Hodgson, HJF},
doi = {10.1111/j.1478-3231.2004.0929.x},
journal = {Liver Int},
pages = {354--360},
title = {Characteristics of murine histidinaemia and its potential for genetic manipulation.},
url = {http://dx.doi.org/10.1111/j.1478-3231.2004.0929.x},
volume = {24},
year = {2004}
}

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TY  - JOUR
AB  - BACKGROUND: Histidinaemia is an autosomal recessive disorder affecting the hepatic enzyme histidine ammonia lyase (histidase) resulting in elevated plasma and urinary histidine and is prototypic of a series of hepatic cytosolic enzyme defects. AIMS: To characterise the physiology of murine histidinaemia with respect to histidine excretion and catabolism, and explore the potential for manipulating cellular and whole body histidase metabolism by gene transfer. MATERIALS AND METHODS: We studied his/his mice which have a G to A substitution in the gene encoding histidase, using both in vitro transduction of isolated hepatocytes by lipofection with wild-type histidase cDNA, and in vivo transduction of whole liver using a retroviral construct. RESULTS AND CONCLUSION: Histidase cDNA expression restored histidase activity in vivo and in vitro towards normal levels, demonstrated both at the cellular level and by whole body metabolic studies, establishing the potential of this model for the development of new gene therapeutic approaches.
AU  - Mellor,N
AU  - Themis,M
AU  - Selden,C
AU  - Jones,M
AU  - Hodgson,HJF
DO  - 10.1111/j.1478-3231.2004.0929.x
EP  - 360
PY  - 2004///
SN  - 1478-3223
SP  - 354
TI  - Characteristics of murine histidinaemia and its potential for genetic manipulation.
T2  - Liver Int
UR  - http://dx.doi.org/10.1111/j.1478-3231.2004.0929.x
UR  - https://www.ncbi.nlm.nih.gov/pubmed/15287859
VL  - 24
ER  -

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