Imperial College London
Alternate

ProfessorMarkThursz

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Hepatology. Head of Department
 
 
 
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Contact

 

+44 (0)20 3312 1903m.thursz

 
 
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Assistant

 

Ms Dawn Campbell +44 (0)20 3312 6454

 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ortega-Prieto:2018:10.1038/s41467-018-02969-8,
author = {Ortega-Prieto, AM and Skelton, JK and Wai, SN and Large, E and Lussignol, M and Vizcay-Barrena, G and Hughes, D and Fleck, R and Thursz, M and Catanese, MT and Dorner, M},
doi = {10.1038/s41467-018-02969-8},
journal = {Nature Communications},
title = {3D microfluidic liver cultures as physiological preclinical tool for hepatitis B virus infection},
url = {http://dx.doi.org/10.1038/s41467-018-02969-8},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - With more than 240 million people infected, hepatitis B virus (HBV) is a major health concern. The inability to mimic the complexity of the liver using cell line and regular primary human hepatocyte (PHH) cultures pose significant limitations for studying host/pathogen interactions. Here, we describe a 3D-microfluidic PHH system permissive to HBV infection, which can be maintained for at least 40 days. This system enables the recapitulation of all steps of the HBV life cycle, including the replication of patient-derived HBV and the maintenance of HBV cccDNA. We show that innate immune and cytokine responses following infection with HBV mimic those observed in HBV-infected patients, thus allowing the dissection of pathways important for immune evasion and validation of biomarkers. Additionally, we demonstrate that the co-culture of PHH with other non-parenchymal cells enables the identification of the cellular origin of immune effectors, thus providing a valuable preclinical platform for HBV research.
AU  - Ortega-Prieto,AM
AU  - Skelton,JK
AU  - Wai,SN
AU  - Large,E
AU  - Lussignol,M
AU  - Vizcay-Barrena,G
AU  - Hughes,D
AU  - Fleck,R
AU  - Thursz,M
AU  - Catanese,MT
AU  - Dorner,M
DO  - 10.1038/s41467-018-02969-8
PY  - 2018///
SN  - 2041-1723
TI  - 3D microfluidic liver cultures as physiological preclinical tool for hepatitis B virus infection
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-018-02969-8
UR  - http://hdl.handle.net/10044/1/55649
VL  - 9
ER  -
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