Imperial College London

ProfessorMarinvan Heel

Faculty of Natural SciencesDepartment of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 5316m.vanheel Website

 
 
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Assistant

 

Dr Audrey Geffen +44 (0)20 7594 5323

 
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Location

 

G20Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

89 results found

Vázquez-Fernández E, Vos MR, Afanasyev P, Cebey L, Sevillano AM, Vidal E, Rosa I, Renault L, Ramos A, Peters PJ, Fernández JJ, van Heel M, Young HS, Requena JR, Wille Het al., 2016, The Structural Architecture of an Infectious Mammalian Prion Using Electron Cryomicroscopy, PLOS Pathogens, Vol: 12, ISSN: 1553-7366

The structure of the infectious prion protein (PrPSc), which is responsible for Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy, has escaped all attempts at elucidation due to its insolubility and propensity to aggregate. PrPSc replicates by converting the non-infectious, cellular prion protein (PrPC) into the misfolded, infectious conformer through an unknown mechanism. PrPSc and its N-terminally truncated variant, PrP 27-30, aggregate into amorphous aggregates, 2D crystals, and amyloid fibrils. The structure of these infectious conformers is essential to understanding prion replication and the development of structure-based therapeutic interventions. Here we used the repetitive organization inherent to GPI-anchorless PrP 27-30 amyloid fibrils to analyze their structure via electron cryomicroscopy. Fourier-transform analyses of averaged fibril segments indicate a repeating unit of 19.1 Å. 3D reconstructions of these fibrils revealed two distinct protofilaments, and, together with a molecular volume of 18,990 Å3, predicted the height of each PrP 27-30 molecule as ~17.7 Å. Together, the data indicate a four-rung β-solenoid structure as a key feature for the architecture of infectious mammalian prions. Furthermore, they allow to formulate a molecular mechanism for the replication of prions. Knowledge of the prion structure will provide important insights into the self-propagation mechanisms of protein misfolding.

Journal article

Afanasyev P, Ravelli RBG, Matadeen R, De Carlo S, van Duinen G, Alewijnse B, Peters PJ, Abrahams J-P, Portugal RV, Schatz M, van Heel Met al., 2015, A posteriori correction of camera characteristics from large image data sets, Scientific Reports, Vol: 5, ISSN: 2045-2322

Large datasets are emerging in many fields of image processing including: electron microscopy, light microscopy, medical X-ray imaging, astronomy, etc. Novel computer-controlled instrumentation facilitates the collection of very large datasets containing thousands of individual digital images. In single-particle cryogenic electron microscopy (“cryo-EM”), for example, large datasets are required for achieving quasi-atomic resolution structures of biological complexes. Based on the collected data alone, large datasets allow us to precisely determine the statistical properties of the imaging sensor on a pixel-by-pixel basis, independent of any “a priori” normalization routinely applied to the raw image data during collection (“flat field correction”). Our straightforward “a posteriori” correction yields clean linear images as can be verified by Fourier Ring Correlation (FRC), illustrating the statistical independence of the corrected images over all spatial frequencies. The image sensor characteristics can also be measured continuously and used for correcting upcoming images.

Journal article

van Heel M, 2013, Finding trimeric HIV-1 envelope glycoproteins in random noise, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: E4175-E4177, ISSN: 0027-8424

Journal article

Bebeacua C, Tremblay D, Farenc C, Chapot-Chartier M-P, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau Cet al., 2013, Structure, Adsorption to Host, and Infection Mechanism of Virulent Lactococcal Phage p2, JOURNAL OF VIROLOGY, Vol: 87, Pages: 12302-12312, ISSN: 0022-538X

Journal article

Nederlof I, Li YW, van Heel M, Abrahams JPet al., 2013, Imaging protein three-dimensional nanocrystals with cryo-EM, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Vol: 69, Pages: 852-859, ISSN: 0907-4449

Journal article

Manzine LR, Balasco Serrao VH, Trambaioli da Rocha e Lima LM, de Souza MM, Bettini J, Portugal RV, van Heel M, Thiemann OHet al., 2013, Assembly stoichiometry of bacterial selenocysteine synthase and SelC (tRNA(sec)), FEBS LETTERS, Vol: 587, Pages: 906-911, ISSN: 0014-5793

Journal article

Bebeacua C, Lai L, Vegge CS, Brondsted L, van Heel M, Veesler D, Cambillau Cet al., 2013, Visualizing a Complete Siphoviridae Member by Single-Particle Electron Microscopy: the Structure of Lactococcal Phage TP901-1, JOURNAL OF VIROLOGY, Vol: 87, Pages: 1061-1068, ISSN: 0022-538X

Journal article

Bebeacua C, Bron P, Lai L, Vegge CS, Brondsted L, Spinelli S, Campanacci V, Veesler D, van Heel M, Cambillau Cet al., 2010, Structure and Molecular Assignment of Lactococcal Phage TP901-1 Baseplate, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 39079-39086

Journal article

Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau Cet al., 2010, Structure of lactococcal phage p2 baseplate and its mechanism of activation, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 6852-6857, ISSN: 0027-8424

Journal article

Baier J, Gabrielsen M, Oellerich S, Michel H, van Heel M, Cogdell RJ, Koehler Jet al., 2009, Spectral Diffusion and Electron-Phonon Coupling of the B800 BChl a Molecules in LH2 Complexes from Three Different Species of Purple Bacteria, BIOPHYSICAL JOURNAL, Vol: 97, Pages: 2604-2612, ISSN: 0006-3495

Journal article

Marles-Wright J, Grant T, Delumeau O, van Duinen G, Firbank SJ, Lewis PJ, Murray JW, Newman JA, Quin MB, Race PR, Rohou A, Tichelaar W, van Heel M, Lewis RJet al., 2008, Molecular architecture of the "stressosome," a signal integration and transduction hub, SCIENCE, Vol: 322, Pages: 92-96, ISSN: 0036-8075

Journal article

Hildner R, Lemmer U, Scherf U, van Heel M, Koehler Jet al., 2007, Revealing the electron-phonon coupling in a conjugated polymer by single-molecule spectroscopy, ADVANCED MATERIALS, Vol: 19, Pages: 1978-+, ISSN: 0935-9648

Journal article

Costa A, Pape T, van Heel M, Brick P, Patwardhan A, Onesti Set al., 2006, Structural basis of the Methanothermobacter thermautotrophicus MCM helicase activity, NUCLEIC ACIDS RESEARCH, Vol: 34, Pages: 5829-5838, ISSN: 0305-1048

Journal article

Costa A, Pape T, van Heel M, Brick P, Patwardhan A, Onesti Set al., 2006, Structural studies of the archaeal MCM complex in different functional states, JOURNAL OF STRUCTURAL BIOLOGY, Vol: 156, Pages: 210-219, ISSN: 1047-8477

Journal article

Ray P, Hall RJ, Finn RD, Chen SX, Patwardhan A, Buck M, van Heel Met al., 2005, Conformational changes of Escherichia coli sigma(54)-RNA-polymerase upon closed-promoter complex formation, JOURNAL OF MOLECULAR BIOLOGY, Vol: 354, Pages: 201-205, ISSN: 0022-2836

Journal article

van Duinen G, van Heel M, Patwardhan A, 2005, Magnification variations due to illumination curvature and object defocus in transmission electron microscopy, OPTICS EXPRESS, Vol: 13, Pages: 9085-9093, ISSN: 1094-4087

Journal article

van Heel M, Schatz M, 2005, Fourier shell correlation threshold criteria, JOURNAL OF STRUCTURAL BIOLOGY, Vol: 151, Pages: 250-262, ISSN: 1047-8477

Journal article

Hofmann C, Michel H, van Heel M, Kohler Jet al., 2005, Multivariate analysis of single-molecule spectra: Surpassing spectral diffusion, PHYSICAL REVIEW LETTERS, Vol: 94, ISSN: 0031-9007

Journal article

Klaholz BP, Myasnikov AG, van Heel M, 2004, Visualization of release factor 3 on the ribosome during termination of protein synthesis, NATURE, Vol: 427, Pages: 862-865, ISSN: 0028-0836

Journal article

Plaisier JR, Koning RI, Koerten HK, van Heel M, Abrahams JPet al., 2004, TYSON: Robust searching, sorting, and selecting of single particles in electron micrographs, JOURNAL OF STRUCTURAL BIOLOGY, Vol: 145, Pages: 76-83, ISSN: 1047-8477

Journal article

Pape T, Meka H, Chen SX, Vicentini G, van Heel M, Onesti Set al., 2003, Hexameric ring structure of the full-length archaeal MCM protein complex, EMBO REPORTS, Vol: 4, Pages: 1079-1083, ISSN: 1469-221X

Journal article

Orlova EV, Gowen B, Droge A, Stiege A, Weise F, Lurz R, van Heel M, Tavares Pet al., 2003, Structure of a viral DNA gatekeeper at 10 angstrom resolution by cryo-electron microscopy, EMBO JOURNAL, Vol: 22, Pages: 1255-1262, ISSN: 0261-4189

Journal article

Orlova EV, Papakosta M, Booy FP, van Heel M, Dolly JOet al., 2003, Voltage-gated K+ channel from mammalian brain: 3D structure at 18 angstrom of the complete (alpha)(4)(beta)(4) complex, JOURNAL OF MOLECULAR BIOLOGY, Vol: 326, Pages: 1005-1012, ISSN: 0022-2836

Journal article

Klaholz BP, Pape T, Zavialov AV, Myasnikov AG, Orlova EV, Vestergaard B, Ehrenberg M, van Heel Met al., 2003, Structure of the Escherichia coli ribosomal termination complex with release factor 2, NATURE, Vol: 421, Pages: 90-94, ISSN: 0028-0836

Journal article

Ray P, Klaholz BP, Finn RD, Orlova EV, Burrows PC, Gowen B, Buck M, Van Heel Met al., 2003, Determination of Escherichia coli RNA polymerase structure by single particle cryoelectron microscopy, RNA POLYMERASES AND ASSOCIATED FACTORS, PT C, Vol: 370, Pages: 24-+, ISSN: 0076-6879

Journal article

Ray P, Klaholz BP, Finn RD, Orlova EV, Burrows PC, Gowen B, Buck M, van Heel Met al., 2003, Determination of Escherichia coli RNA polymerase structure by single particle cryoelectron microscopy., Methods Enzymol, Vol: 370, Pages: 24-42, ISSN: 0076-6879

Journal article

Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel Met al., 2002, Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex, NATURE STRUCTURAL BIOLOGY, Vol: 9, Pages: 849-854, ISSN: 1072-8368

Journal article

Finn RD, Orlova EV, van Heel M, Buck Met al., 2002, Structures of multisubunit DNA-dependent RNA polymerases, 61st Symposium of the Society-for-General-Microbiology, Publisher: CAMBRIDGE UNIV PRESS, Pages: 73-103, ISSN: 0081-1394

Conference paper

Lurz R, Orlova EV, Gunther D, Dube P, Droge A, Weise F, van Heel M, Tavares Pet al., 2001, Structural organisation of the head-to-tail interface of a bacterial virus, JOURNAL OF MOLECULAR BIOLOGY, Vol: 310, Pages: 1027-1037, ISSN: 0022-2836

Journal article

Matadeen R, Sergiev P, Leonov A, Pape T, van der Sluis E, Mueller F, Osswald M, von Knoblauch K, Brimacombe R, Bogdanov A, van Heel M, Dontsova Oet al., 2001, Direct localization by cryo-electron microscopy of secondary structural elements in Escherichia coli 23 S rRNA which differ from the corresponding regions in Haloarcula marismortui, JOURNAL OF MOLECULAR BIOLOGY, Vol: 307, Pages: 1341-1349, ISSN: 0022-2836

Journal article

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