Imperial College London
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Professor Martin Wilkins

Faculty of Medicine, National Heart & Lung Institute

Professor of Clinical Pharmacology
 
 
 
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Contact

 

+44 (0)20 3313 6101m.wilkins Website

 
 
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Assistant

 

Mrs Elizabeth O'Brien +44 (0)20 3313 6101

 
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Location

 

NIHR Imperial Clinical Research FacilityICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhao:1996,
author = {Zhao, L and Brown, LA and Owji, AA and Nunez, DJR and Smith, DM and Ghatei, MA and Bloom, SR and Wilkins, MR},
journal = {American Journal of Physiology - Heart and Circulatory Physiology},
title = {Adrenomedullin activity in chronically hypoxic rat lungs},
volume = {40},
year = {1996}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Adrenomedullin (AM) is a novel vasodilator with structural similarities to calcitonin gene-related peptide (CGRP). This study investigated AM activity in the rat lung during hypoxia-induced pulmonary hypertension. Both rat AM (0.2-10 nmol) and α-CGRP (0.2-2 nmol) produced dose-related reductions in pulmonary artery pressure in the isolated perfused lung ventilated with 2% O 2 . Pretreatment with α-CGRP, which demonstrated tachyphylaxis, or its antagonist, CGRP-(8-37), reduced the hypotensive response to AM, suggesting that part of the response to AM is mediated by CGRP receptors. 125 I-labeled AM and 125 I-labeled CGRP binding was significantly increased in lung membranes from 7-day hypoxic animals (AM from 1.94 ± 0.3 to 3.36 ± 0.4 and CGRP from 0.06 ± 0.01 to 0.12 ± 0.02 pmol/mg protein), with no change in dissociation constant. Moreover, the hypotensive response to both peptides was increased in the lungs of 7-day hypoxic rats. There was no significant change in lung immunoreactive AM concentrations (hypoxic 5.04 ± 0.48 vs. control 6.28 ± 0.76 pmol/g wet wt of tissue) or steady-state AM mRNA levels in 7-day hypoxic rats. Nonetheless, AM may be useful for the acute pharmacological manipulation of pulmonary artery pressure in hypoxiainduced pulmonary hypertension. Copyright © 1996 the American Physiological Society.
AU  - Zhao,L
AU  - Brown,LA
AU  - Owji,AA
AU  - Nunez,DJR
AU  - Smith,DM
AU  - Ghatei,MA
AU  - Bloom,SR
AU  - Wilkins,MR
PY  - 1996///
SN  - 0363-6135
TI  - Adrenomedullin activity in chronically hypoxic rat lungs
T2  - American Journal of Physiology - Heart and Circulatory Physiology
VL  - 40
ER  -
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