Imperial College London
Alternate

Professor SirMagdiYacoub

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor
 
 
 
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Contact

 

+44 (0)1895 828 893m.yacoub

 
 
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Location

 

Heart Science Centre, HarefieldHarefield HospitalHarefield Hospital

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Summary

 

Publications

Citation

BibTex format

@article{Kutryb-Zajac:2016:10.1007/s12265-016-9672-6,
author = {Kutryb-Zajac, B and Yuen, AHY and Khalpey, Z and Zukowska, P and Slominska, EM and Taylor, PM and Goldstein, S and Heacox, AE and Lavitrano, M and Chester, AH and Yacoub, MH and Smolenski, RT},
doi = {10.1007/s12265-016-9672-6},
journal = {Journal of Cardiovascular Translational Research},
pages = {119--126},
title = {Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies},
url = {http://dx.doi.org/10.1007/s12265-016-9672-6},
volume = {9},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to <10 %. Detergent decellularization completely removed (<5 %) all these activities. These results demonstrate high intensity of extracellular nucleotide metabolism on valve surface and indicate that various valve decellularization techniques differently affect ecto-enzyme activities that could be important in the development of improved valve prostheses.
AU  - Kutryb-Zajac,B
AU  - Yuen,AHY
AU  - Khalpey,Z
AU  - Zukowska,P
AU  - Slominska,EM
AU  - Taylor,PM
AU  - Goldstein,S
AU  - Heacox,AE
AU  - Lavitrano,M
AU  - Chester,AH
AU  - Yacoub,MH
AU  - Smolenski,RT
DO  - 10.1007/s12265-016-9672-6
EP  - 126
PY  - 2016///
SN  - 1937-5395
SP  - 119
TI  - Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies
T2  - Journal of Cardiovascular Translational Research
UR  - http://dx.doi.org/10.1007/s12265-016-9672-6
UR  - http://hdl.handle.net/10044/1/34645
VL  - 9
ER  -
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