Imperial College London
Alternate

Professor SirMagdiYacoub

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor
 
 
 
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Contact

 

+44 (0)1895 828 893m.yacoub

 
 
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Location

 

Heart Science Centre, HarefieldHarefield HospitalHarefield Hospital

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Summary

 

Publications

Citation

BibTex format

@article{Bednarczuk-Kaniewska:2016:10.1080/15257770.2016.1163377,
author = {Bednarczuk-Kaniewska, E and Mielcarek, M and Chester, AH and Slominska, EM and Yacoub, MH and Smolenski, RT},
doi = {10.1080/15257770.2016.1163377},
journal = {Nucleosides, Nucleotides and Nucleic Acids},
pages = {713--719},
title = {Oxidized low-density lipoproteins enhance expression and activity of CD39 and CD73 in the human aortic valve endothelium.},
url = {http://dx.doi.org/10.1080/15257770.2016.1163377},
volume = {35},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Extracellular nucleotides regulate thrombosis, inflammation and immune response.Ectonucleoside triphosphate diphosphohydrolase 1 (CD39) and ecto-5'-nucleotidase (CD73)convert extracellular nucleotides in a sequential order: ATP to ADP, AMP and then toadenosine. In this study, we aimed to test an effect of oxidized LDL (ox-LDL) on CD39 andCD73 in endothelial cells.Human aortic valve endothelial cells were exposed to oxidized low-densitylipoprotein, for 24-48 h. Next, the activity, protein expression and mRNA transcripts level ofCD39 and CD73 were characterised by: an incubation with ATP or AMP followed by HPLCanalysis of media as well as western blots and qPCR. Results are presented as mean ± SEM.CD73 activity in human valve endothelial cells was increased in presence of ox-LDL(4.04±0.32 nmol/mg prot./min) as compared to control (2.75±0.21 nmol/mg prot/min). Therewas almost no effect of ox-LDL on CD39 activity. A similar effect was observed for mRNAand protein expression.In conclusion, we found that ox-LDL modulated CD39 and CD73 activity in theendothelium, which may contribute to relevant pathologies and featured treatments.
AU  - Bednarczuk-Kaniewska,E
AU  - Mielcarek,M
AU  - Chester,AH
AU  - Slominska,EM
AU  - Yacoub,MH
AU  - Smolenski,RT
DO  - 10.1080/15257770.2016.1163377
EP  - 719
PY  - 2016///
SN  - 1525-7770
SP  - 713
TI  - Oxidized low-density lipoproteins enhance expression and activity of CD39 and CD73 in the human aortic valve endothelium.
T2  - Nucleosides, Nucleotides and Nucleic Acids
UR  - http://dx.doi.org/10.1080/15257770.2016.1163377
UR  - http://hdl.handle.net/10044/1/32655
VL  - 35
ER  -
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