Imperial College London
Alternate

Professor SirMagdiYacoub

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor
 
 
 
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Contact

 

+44 (0)1895 828 893m.yacoub

 
 
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Location

 

Heart Science Centre, HarefieldHarefield HospitalHarefield Hospital

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Summary

 

Publications

Citation

BibTex format

@article{van:2017:10.1016/j.carpath.2017.02.006,
author = {van, Engeland NCA and Bertazzo, S and Sarathchandra, P and McCormack, A and Bouten, CVC and Yacoub, MH and Chester, AH and Latif, N},
doi = {10.1016/j.carpath.2017.02.006},
journal = {Cardiovascular Pathology},
pages = {36--45},
title = {Aortic calcified particles modulate valvular endothelial and interstitial cells.},
url = {http://dx.doi.org/10.1016/j.carpath.2017.02.006},
volume = {28},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - AbstractBackgroundNormal and calcified human valve cusps, coronary arteries, and aortae harbor spherical calcium phosphate microparticles of identical composition and crystallinity, and their role remains unknown.ObjectiveThe objective was to examine the direct effects of isolated calcified particles on human valvular cells.Method and resultsCalcified particles were isolated from healthy and diseased aortae, characterized, quantitated, and applied to valvular endothelial cells (VECs) and interstitial cells (VICs). Cell differentiation, viability, and proliferation were analyzed. Particles were heterogeneous, differing in size and shape, and were crystallized as calcium phosphate. Diseased donors had significantly more calcified particles compared to healthy donors (P<.05), but there were no differences between the composition of the particles from healthy and diseased donors. VECs treated with calcified particles showed a significant decrease in CD31 and VE-cadherin and an increase in von Willebrand factor expression, P<.05. There were significantly increased α-SMA and osteopontin in treated VICs (P<.05), significantly decreased VEC and VIC viability (P<.05), and significantly increased number of terminal deoxynucleotidyl transferase dUTP nick end labeling-positive VECs (P<.05) indicating apoptosis when treated with the calcified particles.ConclusionsIsolated calcified particles from human aortae are not innocent bystanders but induce a phenotypical and pathological change of VECs and VICs characteristic of activated and pathological cells. Therapy tailored to reduce these calcified particles should be investigated.
AU  - van,Engeland NCA
AU  - Bertazzo,S
AU  - Sarathchandra,P
AU  - McCormack,A
AU  - Bouten,CVC
AU  - Yacoub,MH
AU  - Chester,AH
AU  - Latif,N
DO  - 10.1016/j.carpath.2017.02.006
EP  - 45
PY  - 2017///
SN  - 1054-8807
SP  - 36
TI  - Aortic calcified particles modulate valvular endothelial and interstitial cells.
T2  - Cardiovascular Pathology
UR  - http://dx.doi.org/10.1016/j.carpath.2017.02.006
UR  - http://hdl.handle.net/10044/1/60325
VL  - 28
ER  -
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