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@article{Krishnamoorthy:2017:10.1089/ten.TEA.2017.0060,
author = {Krishnamoorthy, N and Tseng, Y-T and Gajendrarao, P and Sarathchandra, P and McCormack, A and Carubelli, I and Sohier, J and Latif, N and Chester, AH and Yacoub, MH},
doi = {10.1089/ten.TEA.2017.0060},
journal = {Tissue Engineering: Parts A, B, and C},
pages = {145--156},
title = {A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering.},
url = {http://dx.doi.org/10.1089/ten.TEA.2017.0060},
volume = {24},
year = {2017}
}

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TY  - JOUR
AB  - The ability of cells to secrete extracellular matrix proteins is an important property in the repair, replacement, and regeneration of living tissue. Cells that populate tissue-engineered constructs need to be able to emulate these functions. The motifs, KTTKS or palmitoyl-KTTKS (peptide amphiphile), have been shown to stimulate production of collagen and fibronectin in differentiated cells. Molecular modeling was used to design different forms of active peptide motifs to enhance the efficacy of peptides to increase collagen and fibronectin production using terminals KTTKS/SKTTK/SKTTKS connected by various hydrophobic linkers, V4A3/V4A2/A4G3. Molecular dynamic simulations showed SKTTKS-V4A3-SKTTKS (P3), with palindromic (SKTTKS) motifs and SKTTK-V4A2-KTTKS (P5), maintained structural integrity and favorable surface electrostatic distributions that are required for functionality. In vitro studies showed that peptides, P3 and P5, showed low toxicity to human adipose-derived stem cells (hADSCs) and significantly increased the production of collagen and fibronectin in a concentration-dependent manner compared with the original active peptide motif. The 4-day treatment showed that stem cell markers of hADSCs remained stable with P3. The molecular design of novel peptides is a promising strategy for the development of intelligent biomaterials to guide stem cell function for tissue engineering applications.
AU  - Krishnamoorthy,N
AU  - Tseng,Y-T
AU  - Gajendrarao,P
AU  - Sarathchandra,P
AU  - McCormack,A
AU  - Carubelli,I
AU  - Sohier,J
AU  - Latif,N
AU  - Chester,AH
AU  - Yacoub,MH
DO  - 10.1089/ten.TEA.2017.0060
EP  - 156
PY  - 2017///
SN  - 1937-3341
SP  - 145
TI  - A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering.
T2  - Tissue Engineering: Parts A, B, and C
UR  - http://dx.doi.org/10.1089/ten.TEA.2017.0060
UR  - http://hdl.handle.net/10044/1/51834
VL  - 24
ER  -

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