Imperial College London

ProfessorMarkNeil

Faculty of Natural SciencesDepartment of Physics

Professor of Photonics
 
 
 
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Contact

 

+44 (0)20 7594 7611mark.neil

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

608Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

262 results found

Görlitz F, Guldbrand S, Runcorn TH, Murray RT, Jaso-Tamame AL, Sinclair HG, Martinez-Perez E, Taylor JR, Neil MAA, Dunsby C, French PMWet al., 2018, easySLM-STED: Stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning., J Biophotonics

We demonstrate a simplified set-up for STED microscopy with a straightforward alignment procedure that uses a single spatial light modulator (SLM) with collinear incident excitation and depletion beams to provide phase modulation of the beam profiles and correction of optical aberrations. We show that this approach can be used to extend the field of view for STED microscopy by correcting chromatic aberration that otherwise leads to walk-off between the focused excitation and depletion beams. We further show how this arrangement can be adapted to increase the imaging speed through multibeam excitation and depletion. Fine adjustments to the alignment can be accomplished using the SLM only, conferring the potential for automation.

JOURNAL ARTICLE

Kim Y, Warren S, Favero F, Stone J, Clegg J, Neil M, Paterson C, Knight J, French P, Dunsby Cet al., 2018, Semi-random multicore fibre design for adaptive multiphoton endoscopy, OPTICS EXPRESS, Vol: 26, Pages: 3661-3673, ISSN: 1094-4087

JOURNAL ARTICLE

Quicke P, Reynolds S, Neil M, Knopfel T, Schultz SR, Foust AJet al., 2018, High speed functional imaging with source localized multifocal two-photon microscopy, BIOMEDICAL OPTICS EXPRESS, Vol: 9, ISSN: 2156-7085

JOURNAL ARTICLE

Sherlock B, Warren SC, Alexandrov Y, Yu F, Stone J, Knight J, Neil MAA, Paterson C, French PMW, Dunsby Cet al., 2018, In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation, JOURNAL OF BIOPHOTONICS, Vol: 11, ISSN: 1864-063X

JOURNAL ARTICLE

Go rlitz F, Corcoran DS, Sparks H, Leitinger B, Neil M, Dunsby C, French Pet al., 2017, Mapping molecular function to biological nanostructure: Combining structured illumination microscopy with fluorescence lifetime imaging

© 2017 OSA. We report the enhancement of spatial resolution and sensitivity of wide-field time-gated imaging and the combination with SIM to map molecular function using FRET to biological nanostructure below the conventional diffraction limit.

CONFERENCE PAPER

Gorlitz F, Corcoran DS, Castano EAG, Leitinger B, Neil MAA, Dunsby C, French PMWet al., 2017, Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM, PHOTONICS, Vol: 4, ISSN: 2304-6732

JOURNAL ARTICLE

Gorlitz F, Kelly DJ, Warren SC, Alibhai D, West L, Kumar S, Alexandrov Y, Munro I, Garcia E, McGinty J, Talbot C, Serwa RA, Thinon E, da Paola V, Murray EJ, Stuhmeier F, Neil MAA, Tate EW, Dunsby C, French PMWet al., 2017, Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X

JOURNAL ARTICLE

Quicke P, Neil M, Knopfel T, Schultz SR, Foust AJet al., 2017, Source-Localized Multifocal Two-Photon Microscopy for High-Speed Functional Imaging, 71st Annual Meeting of the Society-of-General-Physiologists (SGP) on Optical Revolution in Physiology - From Membrane to Brain, Publisher: ROCKEFELLER UNIV PRESS, Pages: 13A-14A, ISSN: 0022-1295

CONFERENCE PAPER

Soltan A, McGovern B, Drakakis E, Neil M, Maaskant P, Akhter M, Lee JS, Degenaar Pet al., 2017, High Density, High Radiance mu LED Matrix for Optogenetic Retinal Prostheses and Planar Neural Stimulation, IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, Vol: 11, Pages: 347-359, ISSN: 1932-4545

JOURNAL ARTICLE

Sparks H, Gorlitz F, Kelly DJ, Warren SC, Kellett PA, Garcia E, Dymoke-Bradshaw AKL, Hares JD, Neil MAA, Dunsby C, French PMWet al., 2017, Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 88, ISSN: 0034-6748

JOURNAL ARTICLE

Cortes E, Huidobro PA, Sinclair HG, Guldbrand S, Peveler WJ, Davies T, Parrinello S, Gorlitz F, Dunsby C, Neil MAA, Sivan Y, Parkin IP, French PMW, Maier SAet al., 2016, Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy, ACS NANO, Vol: 10, Pages: 10454-10461, ISSN: 1936-0851

JOURNAL ARTICLE

Friddin MS, Bolognesi G, Elani Y, Brooks NJ, Law RV, Seddon JM, Neil MAA, Ces Oet al., 2016, Optically assembled droplet interface bilayer (OptiDIB) networks from cell-sized microdroplets, SOFT MATTER, Vol: 12, Pages: 7731-7734, ISSN: 1744-683X

JOURNAL ARTICLE

Friddin MS, Bolognesi G, Elani Y, Brooks NJ, Law RV, Seddon JM, Neil MAA, Ces Oet al., 2016, Light-driven drag and drop assembly of micron-scale bilayer networks for synthetic biology, Pages: 545-546

We have developed a new method to assemble single- or multi-layered networks of droplet interface bilayers (DIBs) from cell-sized droplets using a single beam optical trap (optical tweezers). The novelty of our approach is the ability to directly trap the microdroplets with the laser and manipulate them in 3D to construct DIB networks of user-defined architectures. Our method does not require a complex optical setup, is versatile, contactless, benefits from both high spatial and temporal resolution, and could set a new paradigm for the assembly of smart, synthetic biosystems.

CONFERENCE PAPER

Kim Y, Warren SC, Stone JM, Knight JC, Neil MAA, Paterson C, Dunsby CW, French PMWet al., 2016, Adaptive Multiphoton Endomicroscope Incorporating a Polarization-Maintaining Multicore Optical Fibre, IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, Vol: 22, ISSN: 1077-260X

JOURNAL ARTICLE

Kwakwa K, Savell A, Davies T, Munro I, Parrinello S, Purbhoo MA, Dunsby C, Neil MAA, French PMWet al., 2016, easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 948-957, ISSN: 1864-063X

JOURNAL ARTICLE

McGinty J, Talbot C, Owen D, Grant D, Kumar S, Galletly N, Treanor B, Kennedy G, Lanigan PMP, Munro I, Elson DS, Magee A, Davis D, Stamp G, Neil M, Dunsby C, French PMWet al., 2016, Fluorescence lifetime imaging: Microscopy, endoscopy, and tomography, Handbook of Biomedical Optics, Pages: 589-615, ISBN: 9781420090376

BOOK CHAPTER

Sherlock B, Yu F, Stone J, Warren S, Paterson C, Neil MAA, French PMW, Knight J, Dunsby Cet al., 2016, Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 715-720, ISSN: 1864-063X

JOURNAL ARTICLE

Warren SC, Kim Y, Stone JM, Mitchell C, Knight JC, Neil MAA, Paterson C, French PMW, Dunsby Cet al., 2016, Adaptive multiphoton endomicroscopy through a dynamically deformed multicore optical fiber using proximal detection, OPTICS EXPRESS, Vol: 24, Pages: 21474-21484, ISSN: 1094-4087

JOURNAL ARTICLE

Bolognesi G, Hargreaves A, Ward AD, Kirby AK, Neil M, Bain CD, Ces Oet al., 2015, Microfluidic generation and optical manipulation of ultra-low interfacial tension droplets, Conference on Integrated Photonics - Materials, Devices, and Applications III, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Casey DR, Wylie D, Gallo J, Dent MR, Salehi-Reyhani A, Wilson R, Brooks NJ, Long NJ, Willison KR, Klug DR, Neil MAA, Neale SL, Cooper JM, Ces Oet al., 2015, A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution

© OSA 2015. We demonstrate controllable poration within ≈1 μm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.

CONFERENCE PAPER

Kelly DJ, Warren SC, Alibhai D, Kumar S, Alexandrov Y, Munro I, Margineanu A, McCormack J, Welsh NJ, Serwa RA, Thinon E, Kongsema M, McGinty J, Talbot C, Murray EJ, Stuhmeier F, Neil MAA, Tate EW, Braga VMM, Lam EW-F, Dunsby C, French PMWet al., 2015, Automated multiwell fluorescence lifetime imaging for Forster resonance energy transfer assays and high content analysis, ANALYTICAL METHODS, Vol: 7, Pages: 4071-4089, ISSN: 1759-9660

JOURNAL ARTICLE

Koleva MV, Rothery S, Spitaler M, Neil MAA, Magee AIet al., 2015, Sonic hedgehog multimerization: A self-organizing event driven by post-translational modifications?, MOLECULAR MEMBRANE BIOLOGY, Vol: 32, Pages: 65-74, ISSN: 0968-7688

JOURNAL ARTICLE

Sherlock B, Warren S, Stone J, Neil M, Paterson C, Knight J, French P, Dunsby Cet al., 2015, Fibre-coupled multiphoton microscope with adaptive motion compensation, BIOMEDICAL OPTICS EXPRESS, Vol: 6, Pages: 1876-1884, ISSN: 2156-7085

JOURNAL ARTICLE

Talbot CB, Lagarto J, Warren S, Neil MAA, French PMW, Dunsby Cet al., 2015, Correction Approach for Delta Function Convolution Model Fitting of Fluorescence Decay Data in the Case of a Monoexponential Reference Fluorophore, JOURNAL OF FLUORESCENCE, Vol: 25, Pages: 1169-1182, ISSN: 1053-0509

JOURNAL ARTICLE

Willison KR, Salehi-Reyhani A, Burgin E, Barclay M, Brown A, Neil MA, Ces O, Klug DRet al., 2015, Absolute quantification of protein copy number in single cells using single molecule microarrays, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S179-S179, ISSN: 0175-7571

JOURNAL ARTICLE

Burgin E, Salehi-Reyhani A, Barclay M, Brown A, Kaplinsky J, Novakova M, Neil MAA, Ces O, Willison KR, Klug DRet al., 2014, Absolute quantification of protein copy number using a single-molecule-sensitive microarray, ANALYST, Vol: 139, Pages: 3235-3244, ISSN: 0003-2654

JOURNAL ARTICLE

Lenz MO, Sinclair HG, Savell A, Clegg JH, Brown ACN, Davis DM, Dunsby C, Neil MAA, French PMWet al., 2014, 3-D stimulated emission depletion microscopy with programmable aberration correction, JOURNAL OF BIOPHOTONICS, Vol: 7, Pages: 29-36, ISSN: 1864-063X

JOURNAL ARTICLE

Robinson T, Valluri P, Kennedy G, Sardini A, Dunsby C, Neil MAA, Baldwin GS, French PMW, de Mello AJet al., 2014, Analysis of DNA Binding and Nucleotide Flipping Kinetics Using Two-Color Two-Photon Fluorescence Lifetime Imaging Microscopy, ANALYTICAL CHEMISTRY, Vol: 86, Pages: 10732-10740, ISSN: 0003-2700

JOURNAL ARTICLE

Salehi-Reyhani A, Sharma S, Burgin E, Barclay M, Cass A, Neil MAA, Ces O, Willison KR, Klug DR, Brown A, Novakova Met al., 2014, Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis (vol 13, pg 2066, 2013), LAB ON A CHIP, Vol: 14, Pages: 3430-3430, ISSN: 1473-0197

JOURNAL ARTICLE

Schrems A, Phillips J, Casey D, Wylie D, Novakova M, Sleytr UB, Klug D, Neil MAA, Schuster B, Ces Oet al., 2014, The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells, ANALYST, Vol: 139, Pages: 3296-3304, ISSN: 0003-2654

JOURNAL ARTICLE

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