262 results found
Görlitz F, Guldbrand S, Runcorn TH, et al., 2018, easySLM-STED: Stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning., J Biophotonics
We demonstrate a simplified set-up for STED microscopy with a straightforward alignment procedure that uses a single spatial light modulator (SLM) with collinear incident excitation and depletion beams to provide phase modulation of the beam profiles and correction of optical aberrations. We show that this approach can be used to extend the field of view for STED microscopy by correcting chromatic aberration that otherwise leads to walk-off between the focused excitation and depletion beams. We further show how this arrangement can be adapted to increase the imaging speed through multibeam excitation and depletion. Fine adjustments to the alignment can be accomplished using the SLM only, conferring the potential for automation.
Kim Y, Warren S, Favero F, et al., 2018, Semi-random multicore fibre design for adaptive multiphoton endoscopy, OPTICS EXPRESS, Vol: 26, Pages: 3661-3673, ISSN: 1094-4087
Quicke P, Reynolds S, Neil M, et al., 2018, High speed functional imaging with source localized multifocal two-photon microscopy, BIOMEDICAL OPTICS EXPRESS, Vol: 9, ISSN: 2156-7085
Sherlock B, Warren SC, Alexandrov Y, et al., 2018, In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation, JOURNAL OF BIOPHOTONICS, Vol: 11, ISSN: 1864-063X
Go rlitz F, Corcoran DS, Sparks H, et al., 2017, Mapping molecular function to biological nanostructure: Combining structured illumination microscopy with fluorescence lifetime imaging
© 2017 OSA. We report the enhancement of spatial resolution and sensitivity of wide-field time-gated imaging and the combination with SIM to map molecular function using FRET to biological nanostructure below the conventional diffraction limit.
Gorlitz F, Corcoran DS, Castano EAG, et al., 2017, Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM, PHOTONICS, Vol: 4, ISSN: 2304-6732
Gorlitz F, Kelly DJ, Warren SC, et al., 2017, Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
Quicke P, Neil M, Knopfel T, et al., 2017, Source-Localized Multifocal Two-Photon Microscopy for High-Speed Functional Imaging, 71st Annual Meeting of the Society-of-General-Physiologists (SGP) on Optical Revolution in Physiology - From Membrane to Brain, Publisher: ROCKEFELLER UNIV PRESS, Pages: 13A-14A, ISSN: 0022-1295
Soltan A, McGovern B, Drakakis E, et al., 2017, High Density, High Radiance mu LED Matrix for Optogenetic Retinal Prostheses and Planar Neural Stimulation, IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS, Vol: 11, Pages: 347-359, ISSN: 1932-4545
Sparks H, Gorlitz F, Kelly DJ, et al., 2017, Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 88, ISSN: 0034-6748
Cortes E, Huidobro PA, Sinclair HG, et al., 2016, Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy, ACS NANO, Vol: 10, Pages: 10454-10461, ISSN: 1936-0851
Friddin MS, Bolognesi G, Elani Y, et al., 2016, Optically assembled droplet interface bilayer (OptiDIB) networks from cell-sized microdroplets, SOFT MATTER, Vol: 12, Pages: 7731-7734, ISSN: 1744-683X
Friddin MS, Bolognesi G, Elani Y, et al., 2016, Light-driven drag and drop assembly of micron-scale bilayer networks for synthetic biology, Pages: 545-546
We have developed a new method to assemble single- or multi-layered networks of droplet interface bilayers (DIBs) from cell-sized droplets using a single beam optical trap (optical tweezers). The novelty of our approach is the ability to directly trap the microdroplets with the laser and manipulate them in 3D to construct DIB networks of user-defined architectures. Our method does not require a complex optical setup, is versatile, contactless, benefits from both high spatial and temporal resolution, and could set a new paradigm for the assembly of smart, synthetic biosystems.
Kim Y, Warren SC, Stone JM, et al., 2016, Adaptive Multiphoton Endomicroscope Incorporating a Polarization-Maintaining Multicore Optical Fibre, IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, Vol: 22, ISSN: 1077-260X
Kwakwa K, Savell A, Davies T, et al., 2016, easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 948-957, ISSN: 1864-063X
McGinty J, Talbot C, Owen D, et al., 2016, Fluorescence lifetime imaging: Microscopy, endoscopy, and tomography, Handbook of Biomedical Optics, Pages: 589-615, ISBN: 9781420090376
Sherlock B, Yu F, Stone J, et al., 2016, Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 715-720, ISSN: 1864-063X
Warren SC, Kim Y, Stone JM, et al., 2016, Adaptive multiphoton endomicroscopy through a dynamically deformed multicore optical fiber using proximal detection, OPTICS EXPRESS, Vol: 24, Pages: 21474-21484, ISSN: 1094-4087
Bolognesi G, Hargreaves A, Ward AD, et al., 2015, Microfluidic generation and optical manipulation of ultra-low interfacial tension droplets, Conference on Integrated Photonics - Materials, Devices, and Applications III, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Casey DR, Wylie D, Gallo J, et al., 2015, A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution
© OSA 2015. We demonstrate controllable poration within ≈1 μm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.
Kelly DJ, Warren SC, Alibhai D, et al., 2015, Automated multiwell fluorescence lifetime imaging for Forster resonance energy transfer assays and high content analysis, ANALYTICAL METHODS, Vol: 7, Pages: 4071-4089, ISSN: 1759-9660
Koleva MV, Rothery S, Spitaler M, et al., 2015, Sonic hedgehog multimerization: A self-organizing event driven by post-translational modifications?, MOLECULAR MEMBRANE BIOLOGY, Vol: 32, Pages: 65-74, ISSN: 0968-7688
Sherlock B, Warren S, Stone J, et al., 2015, Fibre-coupled multiphoton microscope with adaptive motion compensation, BIOMEDICAL OPTICS EXPRESS, Vol: 6, Pages: 1876-1884, ISSN: 2156-7085
Talbot CB, Lagarto J, Warren S, et al., 2015, Correction Approach for Delta Function Convolution Model Fitting of Fluorescence Decay Data in the Case of a Monoexponential Reference Fluorophore, JOURNAL OF FLUORESCENCE, Vol: 25, Pages: 1169-1182, ISSN: 1053-0509
Willison KR, Salehi-Reyhani A, Burgin E, et al., 2015, Absolute quantification of protein copy number in single cells using single molecule microarrays, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S179-S179, ISSN: 0175-7571
Burgin E, Salehi-Reyhani A, Barclay M, et al., 2014, Absolute quantification of protein copy number using a single-molecule-sensitive microarray, ANALYST, Vol: 139, Pages: 3235-3244, ISSN: 0003-2654
Lenz MO, Sinclair HG, Savell A, et al., 2014, 3-D stimulated emission depletion microscopy with programmable aberration correction, JOURNAL OF BIOPHOTONICS, Vol: 7, Pages: 29-36, ISSN: 1864-063X
Robinson T, Valluri P, Kennedy G, et al., 2014, Analysis of DNA Binding and Nucleotide Flipping Kinetics Using Two-Color Two-Photon Fluorescence Lifetime Imaging Microscopy, ANALYTICAL CHEMISTRY, Vol: 86, Pages: 10732-10740, ISSN: 0003-2700
Salehi-Reyhani A, Sharma S, Burgin E, et al., 2014, Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis (vol 13, pg 2066, 2013), LAB ON A CHIP, Vol: 14, Pages: 3430-3430, ISSN: 1473-0197
Schrems A, Phillips J, Casey D, et al., 2014, The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells, ANALYST, Vol: 139, Pages: 3296-3304, ISSN: 0003-2654
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.