Publications
300 results found
Kennedy GT, Manning HB, Elson DS, et al., 2010, A fluorescence lifetime imaging scanning confocal endomicroscope, JOURNAL OF BIOPHOTONICS, Vol: 3, Pages: 103-107, ISSN: 1864-063X
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- Citations: 35
Robinson T, Manning HB, Dunsby C, et al., 2010, Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics, Conference on Microfluidics, BioMEMS, and Medical Microsystems VIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 1
McGovern B, Grossman N, Berlinguer-Palmini R, et al., 2010, An Optogenetic neural stimulation platform for concurrent induction and recording of neural activity, Conference on Photonic Therapeutics and Diagnostics VI, Publisher: Society of Photo-optical Instrumentation Engineers, ISSN: 0277-786X
Degenaar P, Grossman N, Berlinguer-Palmini R, et al., 2010, Optoelectronic microarrays for retinal prosthesis, IEEE Biomedical Circuits and Systems Conference, BioCAS 2009, Pages: 183-186
McGovern B, Drakakis EM, Neil M, et al., 2010, Individually Addressable Optoelectronic Arrays for Optogenetic Neural Stimulation, IEEE Biomedical Circuits and Systems Conference (BioCAS), Publisher: IEEE, Pages: 170-173
Boruah BR, Neil MAA, 2009, Focal field computation of an arbitrarily polarized beam using fast Fourier transforms, OPTICS COMMUNICATIONS, Vol: 282, Pages: 4660-4667, ISSN: 0030-4018
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- Citations: 41
DeMello AJ, French PMW, Neil MAA, et al., 2009, Optical detection in microfluidics: From the small to the large, Pages: 712-717
Herein we discuss two broad approaches for performing high sensitivity optical detection within microfluidic environments. First, we describe recent work in which fluorescence lifetime imaging has been shown to be a sensitive probe of environmental parameters such as pH, viscosity, molecular concentration and temperature. Additionally, we demonstrate how dynamic fluorescence lifetime imaging can be used to probe mixing dynamics in segmented-flow microfluidic systems. Moreover, we describe recent work at Imperial College London in which semiconducting polymer light emitting diodes and polymer photodetectors are integrated with microfluidic systems to define a novel format for point-of-care diagnostics. ©2009 IEEE.
McGinty J, Requejo-Isidro J, Munro I, et al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727
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- Citations: 19
Lee ACH, Elson DS, Neil MA, et al., 2009, Solid-state semiconductors are better alternatives to arc-lamps for efficient and uniform illumination in minimal access surgery, Scientific Congress of the Royal-College-of-Surgeons-of-Edinburgh/College-of-Surgeons-of-Hong-Kong (RCSED/CSHK), Publisher: SPRINGER, Pages: 518-526, ISSN: 0930-2794
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- Citations: 19
Lee ACH, Elson DS, Neil MA, et al., 2009, Solid-state semiconductors are better alternatives to arc-lamps for efficient and uniform illumination in minimal access surgery., Surg Endosc, Vol: 23, Pages: 518-526
INTRODUCTION: Current arc-lamp illumination systems have a number of technical and ergonomic limitations. White light-emitting diodes (LEDs) are energy-efficient solid-state lighting devices which are small, durable and inexpensive. Their use as an alternative to arc-lamp light sources in minimal access surgery has not been explored. This study aims to develop an LED-based endo-illuminator and to determine its lighting characteristics for use in minimal access surgery. METHODS: We developed an LED endo-illuminator using a white LED mounted at the tip of a steel rod. Offline image analysis was carried out to compare the illuminated field using the LED endo-illuminator or an arc-lamp based endoscope in terms of uniformity, shadow sharpness and overall image intensity. Direct radiometric power measurements in light intensity and stability were obtained. Visual perception of fine details at the peripheral endoscopic field was assessed by 13 subjects using the different illumination systems. RESULTS: Illumination from the LED endo-illuminator was more uniform compared to illumination from an arc-lamp source, especially at the closer distance of 4 cm (0.0006 versus 0.0028 arbitrary units--lower value indicates more uniform illumination). The shadows were also sharper (edge widths of 16 versus 44 pixels for the first edge and 15 versus 61 pixels for the second edge). The overall mean image intensity was higher (127 versus 100 arbitrary units) when using the autoshutter mode despite the lower direct radiometric power, about one tenth of the arc-lamp endoscopic system. The illumination was also more stable with less flickering (0.02% versus 5% of total power in non-DC components). Higher median scores on visual perception was also obtained (237 versus 157, p < 0.001). CONCLUSION: The LED endo-illuminator provides more uniform illumination with sharper shadows, less flickering and better illumination for visual perception than the arc-lamp-based system currently used.
McGinty J, Dunsby C, Auksorius E, et al., 2009, Chapter 4 Multidimensional fluorescence imaging
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- Citations: 3
Benninger RKP, Vanherberghen B, Young S, et al., 2009, Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses, BIOPHYSICAL JOURNAL, Vol: 96, Pages: L13-L15, ISSN: 0006-3495
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- Citations: 25
Schaerli Y, Wootton RC, Robinson T, et al., 2009, Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 302-306, ISSN: 0003-2700
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- Citations: 221
Boruah BR, Neil MAA, 2009, Laser scanning confocal microscope with programmable amplitude, phase, and polarization of the illumination beam, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 80, ISSN: 0034-6748
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- Citations: 21
Lanigan PMP, Ninkovic T, Chan K, et al., 2009, A microfluidic platform for probing single cell plasma membranes using optically trapped Smart Droplet Microtools (SDMs), LAB ON A CHIP, Vol: 9, Pages: 1096-1101, ISSN: 1473-0197
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- Citations: 11
Robinson T, Schaerli Y, Wootton R, et al., 2009, Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging, LAB ON A CHIP, Vol: 9, Pages: 3437-3441, ISSN: 1473-0197
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- Citations: 29
Degenaar P, Grossman N, Berlinguer-Palmini R, et al., 2009, Optoelectronic microarrays for Retinal Prosthesis, IEEE Biomedical Circuits and Systems Conference (BioCAS), Publisher: IEEE, Pages: 185-188
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.
Grossman N, Nikolic K, Poher V, et al., 2009, Photostimulator for Optogenetic Retinal Prosthesis, IEEE EMBS Conference on Neural Engineering, Publisher: IEEE, Pages: 68-71
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, FRET AND FLIM TECHNIQUES, Editors: Gadella, Publisher: ELSEVIER SCIENCE BV, Pages: 133-169, ISBN: 978-0-08-054958-3
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- Citations: 2
Degenaar P, Grossman N, McGovern B, et al., 2009, Seeing the light: a photonic visual prosthesis for the blind, Conference on Photons and Neurons, Publisher: Society of Photo-optical Instrumentation Engineers, ISSN: 0277-786X
Degenaar P, Grossman N, Memon MA, et al., 2009, Optobionic vision-a new genetically enhanced light on retinal prosthesis, Journal of Neural Engineering, Vol: 6, ISSN: 1741-2560
Talbot C, McGinty J, McGhee E, et al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy
We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.
Manning HB, Kennedy GT, Owen DM, et al., 2008, A compact, multidimensional spectrofluorometer exploiting supercontinuum generation, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 494-505, ISSN: 1864-063X
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- Citations: 35
Talbot CB, McGinty J, Grant DM, et al., 2008, High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 514-521, ISSN: 1864-063X
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- Citations: 40
Nadeau VJ, Elson DS, Hanna GB, et al., 2008, Modelling of a laser-pumped light source for endoscopic surgery, ISSN: 0277-786X
A white light source, based on illumination of a yellow phosphor with a fibre-coupled blue-violet diode laser, has been designed and built for use in endoscopic surgery. This narrow light probe can be integrated into a standard laparoscope or inserted into the patient separately via a needle. We present a Monte Carlo model of light scattering and phosphorescence within the phosphor/silicone matrix at the probe tip, and measurements of the colour, intensity, and uniformity of the illumination. Images obtained under illumination with this light source are also presented, demonstrating the improvement in illumination quality over existing endoscopic light sources. This new approach to endoscopic lighting has the advantages of compact design, improved ergonomics, and more uniform illumination in comparison with current technologies. © 2008 SPIE.
Grant DM, Zhang W, McGhee EJ, et al., 2008, Multiplexed FRET to Image Multiple Signaling Events in Live Cells, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L69-L71, ISSN: 0006-3495
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- Citations: 79
Oddos S, Dunsby C, Purbhoo MA, et al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495
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- Citations: 57
Lanigan PMP, Chan K, Ninkovic T, et al., 2008, Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 5, Pages: S161-S168, ISSN: 1742-5689
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- Citations: 15
McGinty J, Tahir KR, Laine R, et al., 2008, Fluorescence lifetime optical projection tomography, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 390-394, ISSN: 1864-063X
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- Citations: 39
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