Imperial College London
Alternate

Professor MENGXING TANG

Faculty of Engineering, Department of Bioengineering

Professor of Biomedical Imaging
 
 
 
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Contact

 

+44 (0)20 7594 3664mengxing.tang Website

 
 
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Location

 

3.13Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2019:10.1016/j.ultrasmedbio.2019.01.009,
author = {Zhang, G and Lin, S and Leow, CH and Pang, KT and Hernandez, Gil J and Long, N and Eckersley, R and Matsunaga, T and Tang, M},
doi = {10.1016/j.ultrasmedbio.2019.01.009},
journal = {Ultrasound in Medicine and Biology},
pages = {1131--1142},
title = {Quantification of vaporized targeted nanodroplets using high-frame-rate ultrasound and optics},
url = {http://dx.doi.org/10.1016/j.ultrasmedbio.2019.01.009},
volume = {45},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Owing to their ability to efficiently deliver biological cargo and sense the intracellular milieu, vertical arrays of high aspect ratio nanostructures, known as nanoneedles,are being developed as minimally invasive tools for cell manipulation. However, little is known of the mechanisms of cargo transfer across the cell membrane-nanoneedle interface. Particularly,the contributions of membrane piercing, modulation of membrane permeability and endocytosis to cargo transfer remain  largelyunexplored.  Here,   combining   state-of-the-art   electron  and   scanning  ion conductance  microscopy  with  molecular  biology  techniques,  we  show  that  porous  silicon nanoneedle arrays concurrently stimulate independent endocytic pathways which contribute to enhanced biomolecule delivery into human  mesenchymal  stem cells. Electron  microscopy of the  cell  membrane  at nanoneedle  sites  shows  an  intact  lipid  bilayer,  accompanied  by  an accumulation of clathrin-coated pits and caveolae. Nanoneedles enhance the internalisation of biomolecular markers of endocytosis, highlighting the concurrent activation of caveolae-and clathrin-mediated  endocytosis,  alongside  macropinocytosis.  These  events  contribute  to  the nanoneedle-mediated  delivery  (nanoinjection)  of  nucleic  acids  into  human  stem  cells,  which distribute across the cytosol and the endolysosomal system. This data extends the understanding of how nanoneedles modulate biological processes to mediate interaction with the intracellular space, providing indications for the rational design of improved cell-manipulation technologies.
AU  - Zhang,G
AU  - Lin,S
AU  - Leow,CH
AU  - Pang,KT
AU  - Hernandez,Gil J
AU  - Long,N
AU  - Eckersley,R
AU  - Matsunaga,T
AU  - Tang,M
DO  - 10.1016/j.ultrasmedbio.2019.01.009
EP  - 1142
PY  - 2019///
SN  - 0301-5629
SP  - 1131
TI  - Quantification of vaporized targeted nanodroplets using high-frame-rate ultrasound and optics
T2  - Ultrasound in Medicine and Biology
UR  - http://dx.doi.org/10.1016/j.ultrasmedbio.2019.01.009
UR  - http://hdl.handle.net/10044/1/67116
VL  - 45
ER  -
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