Imperial College London
Alternate

Michael A Crawford PhD, FRSB, FRCPath

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

+44 (0)7725 250 541michael.crawford Website CV

 
 
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Location

 

H 3.34Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Msika:2012:10.1016/j.bbalip.2012.04.007,
author = {Msika, O and Brand, A and Crawford, MA and Yavin, E},
doi = {10.1016/j.bbalip.2012.04.007},
journal = {Biochim Biophys Acta},
pages = {1022--1030},
title = {NGF blocks polyunsaturated fatty acids biosynthesis in n-3 fatty acid-supplemented PC12 cells.},
url = {http://dx.doi.org/10.1016/j.bbalip.2012.04.007},
volume = {1821},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Regulation of polyunsaturated fatty acid (PUFA) biosynthesis in proliferating and NGF-differentiated PC12 pheochromocytoma cells deficient in n-3 docosahexaenoic acid (DHA 22:6n-3) was studied. A dose- and time-dependent increase in eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3) and DHA in phosphatidylethanolamine (PtdEtn) and phosphatidylserine (PtdSer) glycerophospholipids (GPL) via the elongation/desaturation pathway following alpha-linolenic acid (ALA, 18:3n-3) supplements was observed. That was accompanied by a marked reduction of eicosatrienoic acid (Mead acid 20:3n-9), an index of PUFA deficiency. EPA supplements were equally effective converted to 22:5n-3 and 22:6n-3. On the other hand, supplements of linoleic acid (LNA, 18:2n-6) were not effectively converted into higher n-6 PUFA intermediates nor did they impair elongation/desaturation of ALA. Co-supplements of DHA along with ALA did not interfere with 20:5n-3 biosynthesis but reduced further elongation to 22-hydrocarbon PUFA intermediates. A marked decrease in the newly synthesized 22:5n-3 and 22:6n-3 following ALA or EPA supplements was observed after nerve growth factor (NGF)-induced differentiation. NGF also inhibited the last step in 22:5n-6 formation from LNA. These results emphasize the importance of overcoming n-3 PUFA deficiency and raise the possibility that growth factor regulation of the last step in PUFA biosynthesis may constitute an important feature of neuronal phenotype acquisition.
AU  - Msika,O
AU  - Brand,A
AU  - Crawford,MA
AU  - Yavin,E
DO  - 10.1016/j.bbalip.2012.04.007
EP  - 1030
PY  - 2012///
SN  - 0006-3002
SP  - 1022
TI  - NGF blocks polyunsaturated fatty acids biosynthesis in n-3 fatty acid-supplemented PC12 cells.
T2  - Biochim Biophys Acta
UR  - http://dx.doi.org/10.1016/j.bbalip.2012.04.007
UR  - https://www.ncbi.nlm.nih.gov/pubmed/22564256
VL  - 1821
ER  -
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