Imperial College London

Dr Nick Brooks

Faculty of Natural SciencesDepartment of Chemistry

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2677n.brooks Website

 
 
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Location

 

207JMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Barlow:2017:10.1038/s41598-017-17883-0,
author = {Barlow, NE and Bolognesi, G and Haylock, S and Flemming, AJ and Brooks, NJ and Barter, LMC and Ces, O and Barlow, NE and Bolognesi, G and Haylock, S and Flemming, AJ and Brooks, NJ and Barter, LMC and Ces, O},
doi = {10.1038/s41598-017-17883-0},
journal = {Scientific Reports},
title = {Rheological Droplet Interface Bilayers (rheo-DIBs): Probing the Unstirred Water Layer Effect on Membrane Permeability via Spinning Disk Induced Shear Stress},
url = {http://dx.doi.org/10.1038/s41598-017-17883-0},
volume = {7},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - A new rheological droplet interface bilayer (rheo-DIB) device is presented as a tool to apply shear stress on biological lipid membranes. Despite their exciting potential for affecting high-throughput membrane translocation studies, permeability assays conducted using DIBs have neglected the effect of the unstirred water layer (UWL). However as demonstrated in this study, neglecting this phenomenon can cause significant underestimates in membrane permeability measurements which in turn limits their ability to predict key processes such as drug translocation rates across lipid membranes. With the use of the rheo-DIB chip, the effective bilayer permeability can be modulated by applying shear stress to the droplet interfaces, inducing flow parallel to the DIB membranes. By analysing the relation between the effective membrane permeability and the applied stress, both the intrinsic membrane permeability and UWL thickness can be determined for the first time using this model membrane approach, thereby unlocking the potential of DIBs for undertaking diffusion assays. The results are also validated with numerical simulations.
AU - Barlow,NE
AU - Bolognesi,G
AU - Haylock,S
AU - Flemming,AJ
AU - Brooks,NJ
AU - Barter,LMC
AU - Ces,O
AU - Barlow,NE
AU - Bolognesi,G
AU - Haylock,S
AU - Flemming,AJ
AU - Brooks,NJ
AU - Barter,LMC
AU - Ces,O
DO - 10.1038/s41598-017-17883-0
PY - 2017///
SN - 2045-2322
TI - Rheological Droplet Interface Bilayers (rheo-DIBs): Probing the Unstirred Water Layer Effect on Membrane Permeability via Spinning Disk Induced Shear Stress
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/s41598-017-17883-0
UR - http://hdl.handle.net/10044/1/54428
VL - 7
ER -