Imperial College London
Alternate

Dr Nick Brooks

Faculty of Natural Sciences, Department of Chemistry

Reader in Membrane Biophysics
 
 
 
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Contact

 

+44 (0)20 7594 2677n.brooks Website

 
 
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Location

 

207JMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Allen:2021:10.1039/d1sm00178g,
author = {Allen, ME and Elani, Y and Brooks, NJ and Seddon, J},
doi = {10.1039/d1sm00178g},
journal = {Soft Matter},
pages = {5763--5771},
title = {The effect of headgroup methylation on polymorphic phase behaviour in hydrated N-methylated phosphoethanolamine: palmitic acid membranes},
url = {http://dx.doi.org/10.1039/d1sm00178g},
volume = {17},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mixtures of fatty acids and phospholipids can form hexagonal (HII) and inverse bicontinuous cubic phases, the latter of which are implicated in various cellular processes and have wide-ranging biotechnological applications in protein crystallisation and drug delivery systems. Therefore, it is vitally important to understand the formation conditions of inverse bicontinuous cubic phases and how their properties can be tuned. We have used differential scanning calorimetry and synchrotron-based small angle and wide angle X-ray scattering (SAXS/WAXS) to investigate the polymorphic phase behaviour of palmitic acid/ partially-methylated phospholipid mixtures, and how headgroup methylation impacts on inverse bicontinuous cubic phase formation. We find that upon partial methylation of the phospholipid headgroup (1 or 2 methyl substituents) inverse bicontinuous cubic phases are formed (of the Im3m spacegroup), which is not the case with 0 or 3 methyl substituents. This shows how important headgroup methylation is for controlling phase behaviour and how a change in headgroup methylation can be used to controllably tune various inverse bicontinuous phase features such as their lattice parameter and the temperature range of their stability.
AU  - Allen,ME
AU  - Elani,Y
AU  - Brooks,NJ
AU  - Seddon,J
DO  - 10.1039/d1sm00178g
EP  - 5771
PY  - 2021///
SN  - 1744-683X
SP  - 5763
TI  - The effect of headgroup methylation on polymorphic phase behaviour in hydrated N-methylated phosphoethanolamine: palmitic acid membranes
T2  - Soft Matter
UR  - http://dx.doi.org/10.1039/d1sm00178g
UR  - https://pubs.rsc.org/en/Content/ArticleLanding/2021/SM/D1SM00178G#!divAbstract
UR  - http://hdl.handle.net/10044/1/88862
VL  - 17
ER  -
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