Imperial College London
Alternate

ProfessorNaomiChayen

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Prof Res Fellow - Professor of Biomedical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 3240n.chayen Website

 
 
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Location

 

Office no. E304Burlington DanesHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

183 results found

Khurshid S, Saridakis E, Govada L, Chayen NEet al., 2014, Porous nucleating agents for protein crystallization, Nature Protocols, Vol: 9, Pages: 1621-1633, ISSN: 1750-2799

Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months.

Journal article

Lansky S, Alalouf O, Solomon V, Alhassid A, Govada L, Chayen NE, Belrhali H, Shoham Y, Shoham Get al., 2014, Crystallization and preliminary crystallographic analysis of Axe2, an acetylxylan esterase from <i>Geobacillus stearothermophilus</i>. (vol 69, pg 430, 2013), ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 70, Pages: 685-685

Journal article

Chayen NE, Shaffer M, Leese H, Govada L, Khurshid S, Mentzel Ret al., 2014, NUCLEANT FOR MACROMOLECULE CRYSTALLISATION.

Patent

Lansky S, Alalouf O, Solomon HV, Alhassid A, Govada L, Chayen NE, Belrhali H, Shoham Y, Shoham Get al., 2014, A unique octameric structure of Axe2, an intracellular acetyl-xylooligosaccharide esterase from <i>Geobacillus stearothermophilus</i>, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Vol: 70, Pages: 261-278, ISSN: 1399-0047

Journal article

Govada L, Saridakis E, Khurshid S, Chayen Net al., 2014, Enhancing the success of crystallization: strategies and techniques, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C1082-C1082, ISSN: 2053-2733

Conference paper

Govada L, Saridakis E, Kassen S, Khurshid S, El-Sharif H, Reddy S, Chayen Net al., 2014, Smart materials for increasing the success of protein crystallization, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C1138-C1138, ISSN: 2053-2733

Conference paper

Chayen N, Reddy SM, 2013, Novel Protein Crystallization Technique, GB2470744

Patent

Solomon HV, Tabachnikov O, Feinberg H, Govada L, Chayen NE, Shoham Y, Shoham Get al., 2013, Crystallization and preliminary crystallographic analysis of GanB, a GH42 intracellular β-galactosidase from <i>Geobacillus stearothermophilus</i>, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 69, Pages: 1114-1119

Journal article

Saridakis E, Chayen NE, 2013, Imprinted polymers assisting protein crystallization, TRENDS IN BIOTECHNOLOGY, Vol: 31, Pages: 515-520, ISSN: 0167-7799

Journal article

Lansky S, Alalouf O, Solomon V, Alhassid A, Govada L, Chayan NE, Belrhali H, Shoham Y, Shoham Get al., 2013, Crystallization and preliminary crystallographic analysis of Axe2, an acetylxylan esterase from <i>Geobacillus stearothermophilus</i>, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 69, Pages: 430-434

Journal article

Kertis F, Khurshid S, Okman O, Kysar JW, Govada L, Chayen N, Erlebacher Jet al., 2012, Heterogeneous nucleation of protein crystals using nanoporous gold nucleants, JOURNAL OF MATERIALS CHEMISTRY, Vol: 22, Pages: 21928-21934, ISSN: 0959-9428

Journal article

Glassford SE, Govada L, Chayen NE, Byrne B, Kazarian SGet al., 2012, Micro ATR FTIR imaging of hanging drop protein crystallisation, VIBRATIONAL SPECTROSCOPY, Vol: 63, Pages: 492-498, ISSN: 0924-2031

Journal article

Reddy SM, Phan QT, El-Sharif H, Govada L, Stevenson D, Chayen NEet al., 2012, Protein Crystallization and Biosensor Applications of Hydrogel-based Molecularly Imprinted Polymers (HydroMIPs), Biomacromolecules, Vol: 13, Pages: 3959-3965

Journal article

Stiegler AL, Draheim KM, Li X, Chayen NE, Calderwood DA, Boggon TJet al., 2012, Structural Basis for Paxillin Binding and Focal Adhesion Targeting of β-Parvin, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, Pages: 32566-32577

Journal article

Mckie AB, Vaughan S, Zanini E, Okon IS, Louis L, de Sousa C, Greene MI, Wang Q, Agarwal R, Shaposhnikov D, Wong JLS, Gungor H, Janczar S, El-Bahrawy M, Lam E-F, Chayen NE, Gabra Het al., 2012, The OPCML Tumor Suppressor Functions as a Cell Surface Repressor–Adaptor, Negatively Regulating Receptor Tyrosine Kinases in Epithelial Ovarian Cancer, Cancer Discovery

Journal article

Stewart PDS, Kolek SA, Briggs RA, Chayen NE, Baldock PFMet al., 2012, Microseed it! A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S142-S142, ISSN: 2053-2733

Conference paper

Chayen NE, 2012, Smart materials for protein crystallization, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S11-S11, ISSN: 2053-2733

Conference paper

Saridakis E, Khurshid S, Govada L, Quan P, Hawkins D, Crichlow GV, Lolis E, Reddy SM, Chayen NEet al., 2011, Protein crystallization facilitated by molecularly imprinted polymers (vol 108, pg 11081, 2011), PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 18566-18566, ISSN: 0027-8424

Journal article

Boudjemline A, Saridakis E, Swann MJ, Govada L, Mavridis IM, Chayen NEet al., 2011, Use of Dual Polarization Interferometry as a Diagnostic Tool for Protein Crystallization, ANALYTICAL CHEMISTRY, Vol: 83, Pages: 7881-7887, ISSN: 0003-2700

Journal article

Stewart PDS, Kolek SA, Briggs RA, Chayen NE, Baldock PFMet al., 2011, Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization, CRYSTAL GROWTH & DESIGN, Vol: 11, Pages: 3432-3441, ISSN: 1528-7483

Journal article

Saridakis E, Khurshid S, Govada L, Phan Q, Hawkins D, Crichlow GV, Lolis E, Reddy SM, Chayen NEet al., 2011, Protein crystallization facilitated by molecularly imprinted polymers, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 11081-11086, ISSN: 0027-8424

Journal article

Abrahams J-P, Apweiler R, Balling R, Bertero MG, Bujnicki JM, Chayen NE, Chene P, Corthals GL, Dylag T, Foerster F, Heck AJR, Henderson PJF, Herwig R, Jehenson P, Kokalj SJ, Laue E, Legrain P, Martens L, Migliorini C, Musacchio A, Podobnik M, Schertler GFX, Schreiber G, Sixma TK, Smit AB, Stuart D, Svergun DI, Taussig MJet al., 2011, "4D Biology for health and disease" workshop report, NEW BIOTECHNOLOGY, Vol: 28, Pages: 291-293, ISSN: 1871-6784

Journal article

Garcia-Caballero A, Gavira JA, Pineda-Molina E, Chayen NE, Govada L, Khurshid S, Saridakis E, Boudjemline A, Swann MJ, Stewart PS, Briggs RA, Kolek SA, Oberthuer D, Dierks K, Betzel C, Santana M, Hobbs JR, Thaw P, Savill TJ, Mesters JR, Hilgenfeld R, Bonander N, Bill RMet al., 2011, Optimization of Protein Crystallization: The OptiCryst Project, CRYSTAL GROWTH & DESIGN, Vol: 11, Pages: 2112-2121, ISSN: 1528-7483

Journal article

McKie AB, Vaughan S, Okon I, Wong JLC, Zanini E, Lam EW-F, Chayen NE, Gabra Het al., 2011, The OPCML tumor suppressor negatively regulates a specific repertoire of 5 receptor tyrosine kinases via a novel proteasomal mechanism, and its recombinant derivative is a potent in-vivo anticancer protein therapy, CANCER RESEARCH, Vol: 71, ISSN: 0008-5472

Journal article

Petit SJ, Wise EL, Chambers JC, Sehmi J, Chayen NE, Kooner JS, Pease JEet al., 2011, The CXCL16 A181V Mutation Selectively Inhibits Monocyte Adhesion to CXCR6 but Is Not Associated With Human Coronary Heart Disease, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 31, Pages: 914-920, ISSN: 1079-5642

Journal article

Kulkarni MV, Tettamanzi MC, Murphy JW, Keeler C, Myszka DG, Chayen NE, Lolis EJ, Hodsdon MEet al., 2010, Two Independent Histidines, One in Human Prolactin and One in Its Receptor, Are Critical for pH-dependent Receptor Recognition and Activation, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 38524-38533

Journal article

Khurshid S, Haire LF, Chayen NE, 2010, Automated seeding for the optimization of crystal quality, JOURNAL OF APPLIED CRYSTALLOGRAPHY, Vol: 43, Pages: 752-756, ISSN: 0021-8898

Journal article

Kertis F, Snyder J, Govada L, Khurshid S, Chayen N, Erlebacher Jet al., 2010, Structure/Processing Relationships in the Fabrication of Nanoporous Gold, JOM, Vol: 62, Pages: 50-56, ISSN: 1047-4838

Journal article

Chayen N, Khurshid S, 2010, Upside Down Protein Crystallization., Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S294-S294, ISSN: 2053-2733

Conference paper

Saridakis E, Boudjemline A, Swann MJ, Mavridis IM, Chayen NEet al., 2010, Dual Polarization Interferometry in Macromolecular Crystallisation Diagnostics., Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S293-S293, ISSN: 2053-2733

Conference paper

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