Publications
183 results found
Boggon TJ, Chayen NE, Snell EH, et al., 1998, Protein crystal movements and fluid flows during microgravity growth, Meeting on Marangoni and Interfacial Phenomena in Materials Processing, Publisher: ROYAL SOC, Pages: 1045-1061, ISSN: 1364-503X
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- Citations: 29
Chayen NE, 1998, Comparative studies of protein crystallization by vapour-diffusion and microbatch techniques, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 54, Pages: 8-15, ISSN: 2059-7983
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- Citations: 110
Chayen NE, 1997, The role of oil in macromolecular crystallization, STRUCTURE, Vol: 5, Pages: 1269-1274, ISSN: 0969-2126
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- Citations: 91
RiesKautt M, Broutin I, Ducruix A, et al., 1997, Crystallogenesis studies in microgravity with the Advanced Protein Crystallization Facility on SpaceHab-01, JOURNAL OF CRYSTAL GROWTH, Vol: 181, Pages: 79-96, ISSN: 0022-0248
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- Citations: 31
Snell EH, Cassetta A, Helliwell JR, et al., 1997, Partial improvement of crystal quality for microgravity-grown apocrustacyanin C1., Acta Crystallogr D Biol Crystallogr, Vol: 53, Pages: 231-239, ISSN: 0907-4449
The protein apocrustacyanin C(1) has been crystallized by vapour diffusion in both microgravity (the NASA space shuttle USML-2 mission) and on the ground. Rocking width measurements were made on the crystals at the ESRF Swiss-Norwegian beamline using a high-resolution psi-circle diffractometer from the University of Karlsruhe. Crystal perfection was then evaluated, from comparison of the reflection rocking curves from a total of five crystals (three grown in microgravity and two earth controls), and by plotting mosaicity versus reflection signal/noise. Comparison was then made with previous measurements of almost 'perfect' lysozyme crystals grown aboard IML-2 and Spacehab-I and reported by Snell et al. [Snell, Weisgerber, Helliwell, Weckert, Hölzer & Schroer (1995). Acta Cryst. D51, 1099-1102]. Overall, the best diffraction-quality apocrustacyanin C(1) crystal was microgravity grown, but one earth-grown crystal was as good as one of the other microgravity-grown crystals. The remaining two crystals (one from microgravity and one from earth) were poorer than the other three and of fairly equal quality. Crystal movement during growth in microgravity, resulting from the use of vapour-diffusion geometry, may be the cause of not realising the 'theoretical' limit of perfect protein crystal quality.
Snell EH, Cassetta A, Helliwell JR, et al., 1997, Partial improvement of crystal quality for microgravity-grown apocrustacyanin C-1, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Vol: 53, Pages: 231-239, ISSN: 0907-4449
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- Citations: 32
Chayen NE, 1997, A novel technique to control the rate of vapour diffusion, giving larger protein crystals, JOURNAL OF APPLIED CRYSTALLOGRAPHY, Vol: 30, Pages: 198-202, ISSN: 0021-8898
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- Citations: 59
Chayen NE, Conti E, Vieille C, et al., 1997, Crystallization and initial X-ray analysis of xylose isomerase from Thermotoga neapolitana, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 53, Pages: 229-230, ISSN: 2059-7983
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- Citations: 10
Chayen NE, Snell EH, Helliwell JR, et al., 1997, CCD video observation of microgravity crystallization: Apocrustacyanin C-1, JOURNAL OF CRYSTAL GROWTH, Vol: 171, Pages: 219-225, ISSN: 0022-0248
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- Citations: 51
Chayen NE, 1996, A novel technique for containerless protein crystallization, PROTEIN ENGINEERING, Vol: 9, Pages: 927-929, ISSN: 0269-2139
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- Citations: 53
Chayen NE, Boggon TJ, Cassetta A, et al., 1996, Trends and challenges in experimental macromolecular crystallography, QUARTERLY REVIEWS OF BIOPHYSICS, Vol: 29, Pages: 227-278, ISSN: 0033-5835
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- Citations: 74
Chayen NE, Gordon EJ, Phillips SE, et al., 1996, Crystallization and initial X-ray analysis of beta-crustacyanin, the dimer of apoproteins A2 and C1, each with a bound astaxanthin molecule., Acta Crystallogr D Biol Crystallogr, Vol: 52, Pages: 409-410, ISSN: 0907-4449
Crystals of beta-crustacyanin, a carotenoid-binding protein from lobster carapace, have been grown under oil from solutions containing sodium potassium phosphate as precipitant. They grow slowly over a period of months to reach maximal dimensions of 0.5 x 0.1 x 0.1 mm, and belong to space group P622 with cell dimensions: a = b = 124.39, c = 188.86 A and gamma = 120 degrees. The crystals diffract to beyond 3 A but are very radiation sensitive, limiting the resolution of usable data. The unit-cell volume suggests that there are two beta-crustacyanin molecules per asymmetric unit.
Chayen NE, Gordon EJ, Phillips SEV, et al., 1996, Crystallization and initial X-ray analysis of beta-crustacyanin, the dimer of apoproteins A(2) and C-1, each with a bound astaxanthin molecule, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 52, Pages: 409-410, ISSN: 2059-7983
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- Citations: 14
Chayen NE, Gordon EJ, Zagalsky PJ, 1996, Crystallization of apocrustacyanin C-1 on the international microgravity laboratory (IML-2) mission, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Vol: 52, Pages: 156-159, ISSN: 0907-4449
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- Citations: 18
Snell EH, Helliwell JR, Cassetta A, et al., 1996, PROTEIN CRYSTAL PERFECTION AND THE CRYSTAL GROWTH PROCESS, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C517-C517, ISSN: 2053-2733
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- Citations: 1
CHAYEN NE, 1995, MICROGRAVITY PROTEIN CRYSTALLIZATION ABOARD THE PHOTON SATELLITE, JOURNAL OF CRYSTAL GROWTH, Vol: 153, Pages: 175-179, ISSN: 0022-0248
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- Citations: 5
BLOW DM, CHAYEN NE, LLOYD LF, et al., 1994, CONTROL OF NUCLEATION OF PROTEIN CRYSTALS, PROTEIN SCIENCE, Vol: 3, Pages: 1638-1643, ISSN: 0961-8368
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- Citations: 70
CHAYEN NE, STEWART PDS, BALDOCK P, 1994, NEW DEVELOPMENTS OF THE IMPAX SMALL-VOLUME AUTOMATED CRYSTALLIZATION SYSTEM, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Vol: 50, Pages: 456-458, ISSN: 0907-4449
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- Citations: 37
CHAYEN NE, ROWLERSON AM, SQUIRE JM, 1993, FISH MUSCLE STRUCTURE - FIBER TYPES IN FLATFISH AND MULLET FIN MUSCLES USING HISTOCHEMISTRY AND ANTIMYOSIN ANTIBODY LABELING, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 14, Pages: 533-542, ISSN: 0142-4319
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- Citations: 17
CHAYEN NE, RADCLIFFE JW, BLOW DM, 1993, CONTROL OF NUCLEATION IN THE CRYSTALLIZATION OF LYSOZYME, PROTEIN SCIENCE, Vol: 2, Pages: 113-118, ISSN: 0961-8368
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- Citations: 41
Chayen NE, Shaw stuart PD, Blow DM, 1992, Microbatch Crystallization Under Oil - A New Technique Allowing Many Small-Volume Crystallization Trials, Journal of Crystal Growth, Vol: 122, Pages: 176-180, ISSN: 0022-0248
WILSON SA, CHAYEN NE, HEMMINGS AM, et al., 1991, CRYSTALLIZATION OF AND PRELIMINARY-X-RAY DATA FOR THE NEGATIVE REGULATOR (AMIC) OF THE AMIDASE OPERON OF PSEUDOMONAS-AERUGINOSA, JOURNAL OF MOLECULAR BIOLOGY, Vol: 222, Pages: 869-871, ISSN: 0022-2836
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- Citations: 12
LLOYD LF, BRICK P, LOU MZ, et al., 1991, MANY CRYSTAL FORMS OF HUMAN-IMMUNODEFICIENCY-VIRUS REVERSE-TRANSCRIPTASE, JOURNAL OF MOLECULAR BIOLOGY, Vol: 217, Pages: 19-22, ISSN: 0022-2836
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- Citations: 20
CHAYEN NE, STEWART PDS, MAEDER DL, et al., 1990, AN AUTOMATED-SYSTEM FOR MICROBATCH PROTEIN CRYSTALLIZATION AND SCREENING, JOURNAL OF APPLIED CRYSTALLOGRAPHY, Vol: 23, Pages: 297-302, ISSN: 0021-8898
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- Citations: 239
CHAYEN NE, LLOYD LF, COLLYER CA, et al., 1989, TRIGONAL CRYSTALS OF GLUCOSE-ISOMERASE REQUIRE THYMOL FOR THEIR GROWTH AND STABILITY, JOURNAL OF CRYSTAL GROWTH, Vol: 97, Pages: 367-374, ISSN: 0022-0248
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- Citations: 11
CHAYEN N, ROWLERSON A, FREUNDLICH A, et al., 1989, FIBER TYPING ON 3 FLATFISH FIN MUSCLES USING HISTOCHEMISTRY AND IMMUNOCYTOCHEMISTRY, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 10, Pages: 172-172, ISSN: 0142-4319
CHAYEN N, AKINS J, CAMPBELLSMITH S, et al., 1988, SOLUBILITY OF GLUCOSE-ISOMERASE IN AMMONIUM-SULFATE SOLUTIONS, JOURNAL OF CRYSTAL GROWTH, Vol: 90, Pages: 112-116, ISSN: 0022-0248
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- Citations: 35
CHAYEN N, FREUNDLICH A, SQUIRE JM, 1987, COMPARATIVE HISTOCHEMISTRY OF A FLATFISH FIN MUSCLE AND OF OTHER VERTEBRATE MUSCLES USED FOR ULTRASTRUCTURAL STUDIES, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 8, Pages: 358-371, ISSN: 0142-4319
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- Citations: 12
CHAYEN N, FREUNDLICH A, SQUIRE JM, 1987, HISTOCHEMICAL FIBER TYPING OF PLAICE FIN MUSCLE, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 8, Pages: 84-84, ISSN: 0142-4319
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- Citations: 1
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