Imperial College London
Alternate

ProfessorNaomiChayen

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Prof Res Fellow - Professor of Biomedical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 3240n.chayen Website

 
 
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Location

 

Office no. E304Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Leese:2016:10.1039/c5sc03595c,
author = {Leese, HS and Govada, L and Saridakis, E and Khurshid, S and Menzel, R and Morishita, T and Clancy, ARJ and White, E and Chayen, NE and Shaffer, MSP},
doi = {10.1039/c5sc03595c},
journal = {Chemical Science},
pages = {2916--2923},
title = {Reductively PEGylated carbon nanomaterials andtheir use to nucleate 3D protein crystals:a comparison of dimensionality},
url = {http://dx.doi.org/10.1039/c5sc03595c},
volume = {7},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A range of carbon nanomaterials, with varying dimensionality, were dispersed by a non-damaging and versatile chemical reduction route, and subsequently grafted by reaction with methoxy polyethylene glycol (mPEG) monobromides. The use of carbon nanomaterials with different geometries provides both a systematic comparison of surface modification chemistry and the opportunity to study factors affecting specific applications. Multi-walled carbon nanotubes, single-walled carbon nanotubes, graphite nanoplatelets, exfoliated few layer graphite and carbon black were functionalized with mPEG-Br, yielding grafting ratios relative to the nanocarbon framework between ca. 7 and 135 wt%; the products were characterised by Raman spectroscopy, TGA-MS, and electron microscopy. The functionalized materials were tested as nucleants by subjecting them to rigorous protein crystallization studies. Sparsely functionalized flat sheet geometries proved exceptionally effective at inducing crystallization of six proteins. This new class of nucleant, based on PEG grafted graphene-related materials, can be widely applied to promote the growth of 3D crystals suitable for X-ray crystallography. The association of the protein ferritin with functionalized exfoliated few layer graphite was directly visualized by transmission electron microscopy, illustrating the formation of ordered clusters of protein molecules critical to successful nucleation.
AU  - Leese,HS
AU  - Govada,L
AU  - Saridakis,E
AU  - Khurshid,S
AU  - Menzel,R
AU  - Morishita,T
AU  - Clancy,ARJ
AU  - White,E
AU  - Chayen,NE
AU  - Shaffer,MSP
DO  - 10.1039/c5sc03595c
EP  - 2923
PY  - 2016///
SN  - 2041-6539
SP  - 2916
TI  - Reductively PEGylated carbon nanomaterials andtheir use to nucleate 3D protein crystals:a comparison of dimensionality
T2  - Chemical Science
UR  - http://dx.doi.org/10.1039/c5sc03595c
UR  - http://hdl.handle.net/10044/1/29138
VL  - 7
ER  -
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