Imperial College London
Alternate

ProfessorNaomiChayen

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Prof Res Fellow - Professor of Biomedical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 3240n.chayen Website

 
 
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Location

 

Office no. E304Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Adams:2018:10.1371/journal.pone.0195010,
author = {Adams, GG and Meal, A and Morgan, PS and Alzahrani, QE and Zobel, H and Lithgo, R and Kok, MS and Besong, DTM and Jiwani, SI and Ballance, S and Harding, SE and Chayen, N and Gillis, RB},
doi = {10.1371/journal.pone.0195010},
journal = {PLoS ONE},
title = {Characterisation of insulin analogues therapeutically available to patients.},
url = {http://dx.doi.org/10.1371/journal.pone.0195010},
volume = {13},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The structure and function of clinical dosage insulin and its analogues were assessed. This included 'native insulins' (human recombinant, bovine, porcine), 'fast-acting analogues' (aspart, glulisine, lispro) and 'slow-acting analogues' (glargine, detemir, degludec). Analytical ultracentrifugation, both sedimentation velocity and equilibrium experiments, were employed to yield distributions of both molar mass and sedimentation coefficient of all nine insulins. Size exclusion chromatography, coupled to multi-angle light scattering, was also used to explore the function of these analogues. On ultracentrifugation analysis, the insulins under investigation were found to be in numerous conformational states, however the majority of insulins were present in a primarily hexameric conformation. This was true for all native insulins and two fast-acting analogues. However, glargine was present as a dimer, detemir was a multi-hexameric system, degludec was a dodecamer (di-hexamer) and glulisine was present as a dimer-hexamer-dihexamer system. However, size-exclusion chromatography showed that the two hexameric fast-acting analogues (aspart and lispro) dissociated into monomers and dimers due to the lack of zinc in the mobile phase. This comprehensive study is the first time all nine insulins have been characterised in this way, the first time that insulin detemir have been studied using analytical ultracentrifugation and the first time that insulins aspart and glulisine have been studied using sedimentation equilibrium. The structure and function of these clinically administered insulins is of critical importance and this research adds novel data to an otherwise complex functional physiological protein.
AU  - Adams,GG
AU  - Meal,A
AU  - Morgan,PS
AU  - Alzahrani,QE
AU  - Zobel,H
AU  - Lithgo,R
AU  - Kok,MS
AU  - Besong,DTM
AU  - Jiwani,SI
AU  - Ballance,S
AU  - Harding,SE
AU  - Chayen,N
AU  - Gillis,RB
DO  - 10.1371/journal.pone.0195010
PY  - 2018///
SN  - 1932-6203
TI  - Characterisation of insulin analogues therapeutically available to patients.
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0195010
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29596514
UR  - http://hdl.handle.net/10044/1/58272
VL  - 13
ER  -
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