Imperial College London
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DrNicholasCroucher

Faculty of Medicine, School of Public Health

Reader in Bacterial Genomics
 
 
 
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Contact

 

+44 (0)20 7594 3820n.croucher

 
 
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Location

 

1104Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Campo:2018:10.7554/eLife.37015,
author = {Campo, JJ and Le, TQ and Pablo, JV and Hung, C and Teng, AA and Tettelin, H and Tate, A and Hanage, WP and Alderson, MR and Liang, X and Malley, R and Lipsitch, M and Croucher, NJ},
doi = {10.7554/eLife.37015},
journal = {Elife},
title = {Panproteome-wide analysis of antibody responses to whole cell pneumococcal vaccination},
url = {http://dx.doi.org/10.7554/eLife.37015},
volume = {7},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Pneumococcal whole cell vaccines (WCVs) could cost-effectively protect against a greater strain diversity than current capsule-based vaccines. Immunoglobulin G (IgG) responses to a WCV were characterised by applying longitudinally-sampled sera, available from 35 adult placebo-controlled phase I trial participants, to a panproteome microarray. Despite individuals maintaining distinctive antibody 'fingerprints', responses were consistent across vaccinated cohorts. Seventy-two functionally distinct proteins were associated with WCV-induced increases in IgG binding. These shared characteristics with naturally immunogenic proteins, being enriched for transporters and cell wall metabolism enzymes, likely unusually exposed on the unencapsulated WCV's surface. Vaccine-induced responses were specific to variants of the diverse PclA, PspC and ZmpB proteins, whereas PspA- and ZmpA-induced antibodies recognised a broader set of alleles. Temporal variation in IgG levels suggested a mixture of anamnestic and novel responses. These reproducible increases in IgG binding a limited, but functionally diverse, set of conserved proteins indicate WCV could provide species-wide immunity.
AU  - Campo,JJ
AU  - Le,TQ
AU  - Pablo,JV
AU  - Hung,C
AU  - Teng,AA
AU  - Tettelin,H
AU  - Tate,A
AU  - Hanage,WP
AU  - Alderson,MR
AU  - Liang,X
AU  - Malley,R
AU  - Lipsitch,M
AU  - Croucher,NJ
DO  - 10.7554/eLife.37015
PY  - 2018///
SN  - 2050-084X
TI  - Panproteome-wide analysis of antibody responses to whole cell pneumococcal vaccination
T2  - Elife
UR  - http://dx.doi.org/10.7554/eLife.37015
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30592459
UR  - http://hdl.handle.net/10044/1/66980
VL  - 7
ER  -
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