Imperial College London
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DrNicholasCroucher

Faculty of Medicine, School of Public Health

Reader in Bacterial Genomics
 
 
 
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Contact

 

+44 (0)20 7594 3820n.croucher

 
 
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Location

 

1104Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lees:2017:10.1099/mgen.0.000103,
author = {Lees, JA and Kremer, PHC and Manso, AS and Croucher, NJ and Ferwerda, B and Serón, MV and Oggioni, MR and Parkhill, J and Brouwer, MC and van, der Ende A and van, de Beek D and Bentley, SD},
doi = {10.1099/mgen.0.000103},
journal = {Microbial Genomics},
title = {Large scale genomic analysis shows no evidence for pathogen adaptation between the blood and cerebrospinal fluid niches during bacterial meningitis.},
url = {http://dx.doi.org/10.1099/mgen.0.000103},
volume = {3},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Recent studies have provided evidence for rapid pathogen genome diversification, some of which could potentially affect the course of disease. We have previously described such variation seen between isolates infecting the blood and cerebrospinal fluid (CSF) of a single patient during a case of bacterial meningitis. Here, we performed whole-genome sequencing of paired isolates from the blood and CSF of 869 meningitis patients to determine whether such variation frequently occurs between these two niches in cases of bacterial meningitis. Using a combination of reference-free variant calling approaches, we show that no genetic adaptation occurs in either invaded niche during bacterial meningitis for two major pathogen species, Streptococcus pneumoniae and Neisseria meningitidis. This study therefore shows that the bacteria capable of causing meningitis are already able to do this upon entering the blood, and no further sequence change is necessary to cross the blood-brain barrier. Our findings place the focus back on bacterial evolution between nasopharyngeal carriage and invasion, or diversity of the host, as likely mechanisms for determining invasiveness.
AU  - Lees,JA
AU  - Kremer,PHC
AU  - Manso,AS
AU  - Croucher,NJ
AU  - Ferwerda,B
AU  - Serón,MV
AU  - Oggioni,MR
AU  - Parkhill,J
AU  - Brouwer,MC
AU  - van,der Ende A
AU  - van,de Beek D
AU  - Bentley,SD
DO  - 10.1099/mgen.0.000103
PY  - 2017///
SN  - 2057-5858
TI  - Large scale genomic analysis shows no evidence for pathogen adaptation between the blood and cerebrospinal fluid niches during bacterial meningitis.
T2  - Microbial Genomics
UR  - http://dx.doi.org/10.1099/mgen.0.000103
UR  - http://hdl.handle.net/10044/1/52705
VL  - 3
ER  -
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