Imperial College London
Portrait Picture

DrNicholasCroucher

Faculty of Medicine, School of Public Health

Reader in Bacterial Genomics
 
 
 
//

Contact

 

+44 (0)20 7594 3820n.croucher

 
 
//

Location

 

1104Sir Michael Uren HubWhite City Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Lees:2017:10.7554/eLife.26255,
author = {Lees, JA and Croucher, NJ and Goldblatt, D and Nosten, F and Parkhill, J and Turner, C and Turner, P and Bentley, SD},
doi = {10.7554/eLife.26255},
journal = {eLife},
title = {Genome-wide identification of lineage and locus specific variation associated with pneumococcal carriage duration},
url = {http://dx.doi.org/10.7554/eLife.26255},
volume = {6},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Streptococcus pneumoniae is a leading cause of invasive disease in infants, especiallyin low-income settings. Asymptomatic carriage in the nasopharynx is a prerequisite for disease, butvariability in its duration is currently only understood at the serotype level. Here we developed amodel to calculate the duration of carriage episodes from longitudinal swab data, and combinedthese results with whole genome sequence data. We estimated that pneumococcal genomicvariation accounted for 63% of the phenotype variation, whereas the host traits considered here(age and previous carriage) accounted for less than 5%. We further partitioned this heritability intoboth lineage and locus effects, and quantified the amount attributable to the largest sources ofvariation in carriage duration: serotype (17%), drug-resistance (9%) and other significant locuseffects (7%). A pan-genome-wide association study identified prophage sequences as beingassociated with decreased carriage duration independent of serotype, potentially by disruption ofthe competence mechanism. These findings support theoretical models of pneumococcalcompetition and antibiotic resistance.
AU  - Lees,JA
AU  - Croucher,NJ
AU  - Goldblatt,D
AU  - Nosten,F
AU  - Parkhill,J
AU  - Turner,C
AU  - Turner,P
AU  - Bentley,SD
DO  - 10.7554/eLife.26255
PY  - 2017///
SN  - 2050-084X
TI  - Genome-wide identification of lineage and locus specific variation associated with pneumococcal carriage duration
T2  - eLife
UR  - http://dx.doi.org/10.7554/eLife.26255
UR  - http://hdl.handle.net/10044/1/54759
VL  - 6
ER  -
Download