Imperial College London

professor neil fairweather

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Microbiology
 
 
 
//

Contact

 

+44 (0)20 7594 5247n.fairweather

 
 
//

Location

 

1.44Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Publication Type
Year
to

125 results found

Peltier J, Shaw HA, Wren BW, Fairweather NFet al., 2017, Disparate subcellular location of putative sortase substrates in Clostridium difficile, SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322

JOURNAL ARTICLE

Buckley AM, Jukes C, Candlish D, Irvine JJ, Spencer J, Fagan RP, Roe AJ, Christie JM, Fairweather NF, Douce GRet al., 2016, Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Serrano M, Crawshaw AD, Dembek M, Monteiro JM, Pereira FC, Pinho MG, Fairweather NF, Salgado PS, Henriques AOet al., 2016, The SpoIIQ-SpoIIIAH complex of Clostridium difficile controls forespore engulfment and late stages of gene expression and spore morphogenesis, MOLECULAR MICROBIOLOGY, Vol: 100, Pages: 204-228, ISSN: 0950-382X

JOURNAL ARTICLE

Charlton TM, Kovacs-Simon A, Michell SL, Fairweather NF, Tate EWet al., 2015, Quantitative Lipoproteomics in Clostridium difficile Reveals a Role for Lipoproteins in Sporulation, CHEMISTRY & BIOLOGY, Vol: 22, Pages: 1562-1573, ISSN: 1074-5521

JOURNAL ARTICLE

Couchman EC, Browne HP, Dunn M, Lawley TD, Songer JG, Hall V, Petrovska L, Vidor C, Awad M, Lyras D, Fairweather NFet al., 2015, Clostridium sordellii genome analysis reveals plasmid localized toxin genes encoded within pathogenicity loci, BMC GENOMICS, Vol: 16, ISSN: 1471-2164

JOURNAL ARTICLE

Dembek M, Barquist L, Boinett CJ, Cain AK, Mayho M, Lawley TD, Fairweather NF, Fagan RPet al., 2015, High-Throughput Analysis of Gene Essentiality and Sporulation in Clostridium difficile, mBio, Vol: 6, ISSN: 2161-2129

Clostridium difficile is the most common cause of antibiotic-associated intestinal infections and a significant cause of morbidity and mortality. Infection with C. difficile requires disruption of the intestinal microbiota, most commonly by antibiotic usage. Therapeutic intervention largely relies on a small number of broad-spectrum antibiotics, which further exacerbate intestinal dysbiosis and leave the patient acutely sensitive to reinfection. Development of novel targeted therapeutic interventions will require a detailed knowledge of essential cellular processes, which represent attractive targets, and species-specific processes, such as bacterial sporulation. Our knowledge of the genetic basis of C. difficile infection has been hampered by a lack of genetic tools, although recent developments have made some headway in addressing this limitation. Here we describe the development of a method for rapidly generating large numbers of transposon mutants in clinically important strains of C. difficile. We validated our transposon mutagenesis approach in a model strain of C. difficile and then generated a comprehensive transposon library in the highly virulent epidemic strain R20291 (027/BI/NAP1) containing more than 70,000 unique mutants. Using transposon-directed insertion site sequencing (TraDIS), we have identified a core set of 404 essential genes, required for growth in vitro. We then applied this technique to the process of sporulation, an absolute requirement for C. difficile transmission and pathogenesis, identifying 798 genes that are likely to impact spore production. The data generated in this study will form a valuable resource for the community and inform future research on this important human pathogen.

JOURNAL ARTICLE

Peltier J, Shaw HA, Couchman EC, Dawson LF, Yu L, Choudhary JS, Kaever V, Wren BW, Fairweather NFet al., 2015, Cyclic diGMP Regulates Production of Sortase Substrates of Clostridium difficile and Their Surface Exposure through Zmpl Protease-mediated Cleavage, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 290, Pages: 24453-24469, ISSN: 0021-9258

JOURNAL ARTICLE

Sekulovic O, Bedoya MO, Fivian-Hughes AS, Fairweather NF, Fortier L-Cet al., 2015, The Clostridium difficile cell wall protein CwpV confers phase-variable phage resistance, MOLECULAR MICROBIOLOGY, Vol: 98, Pages: 329-342, ISSN: 0950-382X

JOURNAL ARTICLE

Willing SE, Candela T, Shaw HA, Seager Z, Mesnage S, Fagan RP, Fairweather NFet al., 2015, Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII, MOLECULAR MICROBIOLOGY, Vol: 96, Pages: 596-608, ISSN: 0950-382X

JOURNAL ARTICLE

Willing SE, Richards EJ, Sempere L, Dale AG, Cutting SM, Fairweather NFet al., 2015, Increased toxin expression in a Clostridium difficile mfd mutant, BMC MICROBIOLOGY, Vol: 15, ISSN: 1471-2180

JOURNAL ARTICLE

Fagan RP, Fairweather NF, 2014, Biogenesis and functions of bacterial S-layers, NATURE REVIEWS MICROBIOLOGY, Vol: 12, Pages: 211-222, ISSN: 1740-1526

JOURNAL ARTICLE

Phetcharaburanin J, Hong HA, Colenutt C, Bianconi I, Sempere L, Permpoonpattana P, Smith K, Dembek M, Tan S, Brisson M-C, Brisson AR, Fairweather NF, Cutting SMet al., 2014, The spore-associated protein BclA1 affects the susceptibility of animals to colonization and infection by Clostridium difficile, MOLECULAR MICROBIOLOGY, Vol: 92, Pages: 1025-1038, ISSN: 0950-382X

JOURNAL ARTICLE

Chen K, d'Arc S, Setty N, Bamford K, Fairweather N, Tyrrell-Price Jet al., 2013, In Recurrent C. difficile, the CRP Response to the Primary C. difficile Infection Predicts Whether the Same Strain or a Different Strain will Cause a Second Infection, DIGESTIVE DISEASES AND SCIENCES, Vol: 58, Pages: 1683-1688, ISSN: 0163-2116

JOURNAL ARTICLE

Dembek M, Stabler RA, Witney AA, Wren BW, Fairweather NFet al., 2013, Transcriptional Analysis of Temporal Gene Expression in Germinating Clostridium difficile 630 Endospores, PLOS ONE, Vol: 8, ISSN: 1932-6203

JOURNAL ARTICLE

Permpoonpattana P, Phetcharaburanin J, Mikelsone A, Dembek M, Tan S, Brisson M-C, La Ragione R, Brisson AR, Fairweather N, Hong HA, Cutting SMet al., 2013, Functional Characterization of Clostridium difficile Spore Coat Proteins, JOURNAL OF BACTERIOLOGY, Vol: 195, Pages: 1492-1503, ISSN: 0021-9193

JOURNAL ARTICLE

Tulli L, Marchi S, Petracca R, Shaw HA, Fairweather NF, Scarselli M, Soriani M, Leuzzi Ret al., 2013, CbpA: a novel surface exposed adhesin of Clostridium difficile targeting human collagen, CELLULAR MICROBIOLOGY, Vol: 15, Pages: 1674-1687, ISSN: 1462-5814

JOURNAL ARTICLE

Dang THT, Fagan RP, Fairweather NF, Tate EWet al., 2012, Novel inhibitors of surface layer processing in Clostridium difficile, BIOORGANIC & MEDICINAL CHEMISTRY, Vol: 20, Pages: 614-621, ISSN: 0968-0896

JOURNAL ARTICLE

Deakin LJ, Clare S, Fagan RP, Dawson LF, Pickard DJ, West MR, Wren BW, Fairweather NF, Dougan G, Lawley TDet al., 2012, The Clostridium difficile spo0A Gene Is a Persistence and Transmission Factor, INFECTION AND IMMUNITY, Vol: 80, Pages: 2704-2711, ISSN: 0019-9567

JOURNAL ARTICLE

Dembek M, Reynolds CB, Fairweather NF, 2012, Clostridium difficile Cell Wall Protein CwpV Undergoes Enzyme-independent Intramolecular Autoproteolysis, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, Pages: 1538-1544, ISSN: 0021-9258

JOURNAL ARTICLE

Tierney R, Nakai T, Parkins CJ, Caposio P, Fairweather NF, Sesardic D, Jarvis MAet al., 2012, A single-dose cytomegalovirus-based vaccine encoding tetanus toxin fragment C induces sustained levels of protective tetanus toxin antibodies in mice, VACCINE, Vol: 30, Pages: 3047-3052, ISSN: 0264-410X

JOURNAL ARTICLE

Fagan RP, Fairweather NF, 2011, Clostridium difficile Has Two Parallel and Essential Sec Secretion Systems, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 27483-27493, ISSN: 0021-9258

JOURNAL ARTICLE

Fagan RP, Janoir C, Collignon A, Mastrantonio P, Poxton IR, Fairweather NFet al., 2011, A proposed nomenclature for cell wall proteins of Clostridium difficile, JOURNAL OF MEDICAL MICROBIOLOGY, Vol: 60, Pages: 1225-1228, ISSN: 0022-2615

JOURNAL ARTICLE

Merrigan M, Venugopal A, Roxas JL, Wiethoff CM, Songer G, Fairweather N, Viswanathan VK, Vedantam Get al., 2011, Clostridium difficile Strains of Human and Veterinary Origin Attach Efficiently to Human Intestinal Epithelial Cells via the Major Surface-Layer Protein Slpa, Conference on Digestive Disease Week 2011, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S636-S636, ISSN: 0016-5085

CONFERENCE PAPER

Permpoonpattana P, Hong HA, Phetcharaburanin J, Huang J-M, Cook J, Fairweather NF, Cutting SMet al., 2011, Immunization with Bacillus Spores Expressing Toxin A Peptide Repeats Protects against Infection with Clostridium difficile Strains Producing Toxins A and B, INFECTION AND IMMUNITY, Vol: 79, Pages: 2295-2302, ISSN: 0019-9567

JOURNAL ARTICLE

Reynolds CB, Emerson JE, de la Riva L, Fagan RP, Fairweather NFet al., 2011, The Clostridium difficile Cell Wall Protein CwpV is Antigenically Variable between Strains, but Exhibits Conserved Aggregation-Promoting Function, PLOS PATHOGENS, Vol: 7, ISSN: 1553-7366

JOURNAL ARTICLE

de la Riva L, Willing SE, Tate EW, Fairweather NFet al., 2011, Roles of Cysteine Proteases Cwp84 and Cwp13 in Biogenesis of the Cell Wall of Clostridium difficile, JOURNAL OF BACTERIOLOGY, Vol: 193, Pages: 3276-3285, ISSN: 0021-9193

JOURNAL ARTICLE

Albesa-Jove D, Bertrand T, Carpenter EP, Swain GV, Lim J, Zhang J, Haire LF, Vasisht N, Braun V, Lange A, von Eichel-Strelber C, Svergun DI, Fairweather NF, Brown KAet al., 2010, Four Distinct Structural Domains in Clostridium difficile Toxin B Visualized Using SAXS, JOURNAL OF MOLECULAR BIOLOGY, Vol: 396, Pages: 1260-1270, ISSN: 0022-2836

JOURNAL ARTICLE

Dang THT, de la Riva L, Fagan RP, Storck EM, Heal WP, Janoir C, Fairweather NF, Tate EWet al., 2010, Chemical Probes of Surface Layer Biogenesis in Clostridium difficile, ACS CHEMICAL BIOLOGY, Vol: 5, Pages: 279-285, ISSN: 1554-8929

JOURNAL ARTICLE

Fagan R, Fairweather N, 2010, Dissecting the cell surface., Methods Mol Biol, Vol: 646, Pages: 117-134

The bacterial cell surface is an important structure as it mediates interactions with the external environment. In the case of pathogens like Clostridium difficile, the cell wall and its components also have to mediate interactions with the host cells and their products. In this chapter we discuss the various methods used for dissecting the cell surface and the biochemical and immunological procedures that are commonly used to analyse the properties of the proteins within the cell wall. A major consideration is the S-layer which in C. difficile shows considerable variation in sequence and between strains, a property which is also reflected in its antigenic properties.

JOURNAL ARTICLE

Robinson KS, Mousnier A, Hemrajani C, Fairweather N, Berger CN, Frankel Get al., 2010, The enteropathogenic Escherichia coli effector NleH inhibits apoptosis induced by Clostridium difficile toxin B, MICROBIOLOGY-SGM, Vol: 156, Pages: 1815-1823, ISSN: 1350-0872

JOURNAL ARTICLE

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

Request URL: http://wlsprd.imperial.ac.uk:80/respub/WEB-INF/jsp/search-html.jsp Request URI: /respub/WEB-INF/jsp/search-html.jsp Query String: respub-action=search.html&id=00159763&limit=30&person=true