Imperial College London

professor neil fairweather

Faculty of Natural SciencesDepartment of Life Sciences

Emeritus Professor
 
 
 
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Contact

 

+44 (0)20 7594 5247n.fairweather

 
 
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Location

 

1.44Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

127 results found

Charles IG, Dougan G, Pickard D, Charfield S, Smith M, Novotny P, Fairweather Net al., 1988, Molecular cloning and analysis of P. 69, a vir-controlled protein from Bordetella pertussis., Tokai J Exp Clin Med, Vol: 13 Suppl, Pages: 227-234, ISSN: 0385-0005

The cloning sequencing and analysis of an important antigenic component of Bordetella pertussis is described. The gene for P.69, in common with a variety of other so called "virulence" genes, (e.g., adenylate cyclase (AC), pertussis toxin (PT) and filamentous haemagglutinin (FHA)), is under control of the vir locus. The protein P.69 is externally localised on cells and protein preparations are protective as judged by the mouse intra-cerebral challenge test. The gene encoding the P.69 antigen was isolated by hybridization of mixed oligonucleotide probes against B. pertussis genomic DNA. These oligonucleotides were designed from the protein sequence data obtained from a cyanogen bromide digest of the P.69 protein. DNA sequence analysis reveals a G:C rich gene capable of encoding a protein of 910 amino acids and Mr of 93478, whose likely promoter and ribosome binding sites show little homology to their E.coli counterpart. In common with some of the genes in the PT operon the sequence 5'-CCTGG-3' was found 5' to the ATG initiation codon. At the 3', end 29 bases after the TAA stop codon, the sequence 5'GTTTTTCCT-3' was found in an equivalent position to the same sequence in the PT operon. Examination of the protein sequence reveals two regions with directly repeated elements (GGAVP)3(GGFGP)2 and (PQP)5.

JOURNAL ARTICLE

FAIRWEATHER NF, LYNESS VA, MASKELL DJ, 1987, IMMUNIZATION OF MICE AGAINST TETANUS WITH FRAGMENTS OF TETANUS TOXIN SYNTHESIZED IN ESCHERICHIA-COLI, INFECTION AND IMMUNITY, Vol: 55, Pages: 2541-2545, ISSN: 0019-9567

JOURNAL ARTICLE

FAIRWEATHER NF, LYNESS VA, 1986, THE COMPLETE NUCLEOTIDE-SEQUENCE OF TETANUS TOXIN, NUCLEIC ACIDS RESEARCH, Vol: 14, Pages: 7809-7812, ISSN: 0305-1048

JOURNAL ARTICLE

FAIRWEATHER NF, LYNESS VA, PICKARD DJ, ALLEN G, THOMSON ROet al., 1986, CLONING, NUCLEOTIDE SEQUENCING, AND EXPRESSION OF TETANUS TOXIN FRAGMENT-C IN ESCHERICHIA-COLI, JOURNAL OF BACTERIOLOGY, Vol: 165, Pages: 21-27, ISSN: 0021-9193

JOURNAL ARTICLE

DOUGAN G, FAIRWEATHER N, 1985, GENETIC-ANALYSIS OF GRAM-POSITIVE TOXIN DETERMINANTS - THE IMPACT OF NEW TECHNOLOGIES, MICROBIOLOGICAL SCIENCES, Vol: 2, Pages: 144-147, ISSN: 0265-1351

JOURNAL ARTICLE

HERRERO E, FAIRWEATHER NF, HOLLAND IB, 1982, ENVELOPE PROTEIN-SYNTHESIS AND INHIBITION OF CELL-DIVISION IN ESCHERICHIA-COLI DURING INACTIVATION OF THE B-SUBUNIT OF DNA GYRASE, JOURNAL OF GENERAL MICROBIOLOGY, Vol: 128, Pages: 361-369, ISSN: 0022-1287

JOURNAL ARTICLE

Fairweather NF, Orr E, Holland IB, 1980, Inhibition of deoxyribonucleic acid gyrase: effects on nucleic acid synthesis and cell division in Escherichia coli K-12., J Bacteriol, Vol: 142, Pages: 153-161, ISSN: 0021-9193

Mutants of Escherichia coli resistant to the antibiotic clorobiocin are also coumermycin resistant, and the mutation to resistance in at least one mutant was mapped near gyrB. We conclude, therefore, that clorobiocin inhibits deoxyribonucleic acid gyrase, and the drug was used to probe the role of this enzyme in vivo. Deozyribonucleic acid synthesis was preferentially inhibited but not completely blocked by the antibiotic. Transcription and cell division were also markedly affected. However, unlike other inhibitors of deoxyribonucleic acid synthesis, clorobiocin failed to induce the synthesis of protein X, the recA gene product. In mutants resistant to clorobiocin the replication velocity was unaffected, but initiation of deoxyribonucleic acid synthesis appeared to be delayed. We conclude that deoxyribonucleic acid gyrase, and hence the supercoiled structure of the chromosome, is important for transcription, normal initiation of deoxyribonucleic acid replication, and cell division. The possible role of deoxyribonucleic acid gyrase in the elongation of replication forks is also discussed.

JOURNAL ARTICLE

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