Imperial College London
Alternate

Emeritus ProfessorNigelGooderham

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Emeritus Professor of Molecular Toxicology
 
 
 
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Contact

 

n.gooderham Website

 
 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Segal:2015:10.1016/j.tox.2015.04.002,
author = {Segal, CV and Koufaris, C and Powell, C and Gooderham, NJ},
doi = {10.1016/j.tox.2015.04.002},
journal = {Toxicology},
pages = {45--52},
title = {Effects of treatment with androgen receptor ligands on microRNA expression of prostate cancer cells},
url = {http://dx.doi.org/10.1016/j.tox.2015.04.002},
volume = {333},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Post-transcriptional regulation by microRNA (miRNA) is an important aspect of androgen receptor (AR) signalling in prostate cancer cells. However, the global profiling of miRNA expression in prostate cancer cells following treatment with AR ligands has not been reported so far. In this study we examined the effect of treatment with two AR agonists (mibolerone (MIB) and dihydrotestosterone (DHT)) and an AR antagonist (bicalutamide (BIC)) on miRNA expression in the human androgen-dependent LNCaP prostate cancer cell line using microarray technology and verification of selected miRNA using quantitative real-time PCR (qRT-PCR). No miRNA was identified as differentially expressed following treatment with the AR antagonist BIC. In contrast, a number of common and compound-specific alterations in miRNA expression were observed following treatment with AR agonists. Unexpectedly it was found that treatment with the AR agonists resulted in the repression of miR-221, a miRNA previously established to be involved with prostate cancer development. This observation indicates that this miRNA may have a more complex role in prostate cancer development than considered previously. Treatment with MIB led to an induction of miR-210 expression, a hypoxia-related miRNA. This miRNA is reported to be involved in cell adaptation to hypoxia and thus induction in conditions of normoxia may be important in driving metabolic changes observed in prostate cancer. Thus examining the effect of AR agonists and antagonists on miRNA expression can provide novel insights into the response of cells to AR ligands and subsequent downstream events.
AU  - Segal,CV
AU  - Koufaris,C
AU  - Powell,C
AU  - Gooderham,NJ
DO  - 10.1016/j.tox.2015.04.002
EP  - 52
PY  - 2015///
SN  - 0300-483X
SP  - 45
TI  - Effects of treatment with androgen receptor ligands on microRNA expression of prostate cancer cells
T2  - Toxicology
UR  - http://dx.doi.org/10.1016/j.tox.2015.04.002
UR  - http://hdl.handle.net/10044/1/21856
VL  - 333
ER  -
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