Imperial College London

ProfessorNicholasGrassly

Faculty of MedicineSchool of Public Health

Prof of Infectious Disease & Vaccine Epidemiology
 
 
 
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Contact

 

+44 (0)20 7594 3264n.grassly Website

 
 
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Location

 

G36Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Parker:2017:10.1016/j.vaccine.2017.11.031,
author = {Parker, EPK and Praharaj, I and Zekavati, A and Lazarus, RP and Giri, S and Operario, DJ and Liu, J and Iturriza-Gómara, M and Kampmann, B and John, J and Kang, G and Grassly, NC},
doi = {10.1016/j.vaccine.2017.11.031},
journal = {Vaccine},
pages = {264--272},
title = {Influence of the intestinal microbiota on the immunogenicity of oral rotavirus vaccine given to infants in south India},
url = {http://dx.doi.org/10.1016/j.vaccine.2017.11.031},
volume = {36},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Oral rotavirus vaccines have consistently proven to be less immunogenic among infants in developing countries. Discrepancies in the intestinal microbiota, including a greater burden of enteropathogens and an altered commensal community composition, may contribute to this trend by inhibiting the replication of vaccine viruses. To test this possibility, we performed a nested case–control study in Vellore, India, in which we compared the intestinal microbiota of infants who responded serologically or not after two doses of Rotarix delivered at 6 and 10weeks of age as part of a clinical trial (CTRI/2012/05/002677). The prevalence of 40 bacterial, viral, and eukaryotic pathogen targets was assessed in pre-vaccination stool samples from 325 infants using singleplex real-time PCR on a Taqman array card (TAC). In a subset of 170 infants, we assessed bacterial microbiota composition by sequencing the 16S rRNA gene V4 region. Contrary to expectations, responders were more likely than non-responders to harbor ≥1 bacterial enteropathogen at dose 1 (26% [40/156] vs 13% [21/157] of infants with TAC results who completed the study per protocol; χ2, P=.006), although this was not apparent at dose 2 (24% [38/158] vs 23% [36/158]; P=.790). Rotavirus shedding after dose 1 was negatively correlated with the replication of co-administered oral poliovirus vaccine (OPV). We observed no consistent differences in composition or diversity of the 16S bacterial microbiota according to serological response, although rotavirus shedding was associated with slightly more bacterial taxa pre-vaccination. Overall, our findings demonstrate an inhibitory effect of co-administered OPV on the first dose of Rotarix, consistent with previous studies, but in the context of OPV co-administration we did not find a strong association between other components of the intestinal microbiota at the time of vaccination and Rotarix immunogenicity.
AU - Parker,EPK
AU - Praharaj,I
AU - Zekavati,A
AU - Lazarus,RP
AU - Giri,S
AU - Operario,DJ
AU - Liu,J
AU - Iturriza-Gómara,M
AU - Kampmann,B
AU - John,J
AU - Kang,G
AU - Grassly,NC
DO - 10.1016/j.vaccine.2017.11.031
EP - 272
PY - 2017///
SN - 0264-410X
SP - 264
TI - Influence of the intestinal microbiota on the immunogenicity of oral rotavirus vaccine given to infants in south India
T2 - Vaccine
UR - http://dx.doi.org/10.1016/j.vaccine.2017.11.031
UR - http://hdl.handle.net/10044/1/53663
VL - 36
ER -